1887

Abstract

The cloning, expression and nucleotide sequence of a 3·74 kb DNA segment on pLS215 containing a -glucosidase gene() from H17c was investigated. The open reading frame (ORF) of 2490 bp encoded a -glucosidase of 830 amino acid residues with a calculated of 91800. In C600(pLS215) cells the -glucosidase was localized in the cytoplasm and these cells produced an additional protein with an apparent of approximately 94000. The gene was expressed from its own regulatory region in and a single mRNA initiation point was identified upstream of the ORF and adjacent to a promoter consensus sequence. The primary structure of the -glucosidase showed > 40 % similarity with a domain of 237 amino acids present in the -glucosidases of and . The -glucosidase hydrolysed cellobiose to a limited extent, cellotriose to cellobiose and glucose, and cellotetraose and cellopentaose to predominantly glucose.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-8-1567
1990-08-01
2022-01-20
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/8/mic-136-8-1567.html?itemId=/content/journal/micro/10.1099/00221287-136-8-1567&mimeType=html&fmt=ahah

References

  1. Aiba H., Adhya S., De crombrugghe B. 1981; Evidence for two functional gal promoters in intact Escherichia coli cells. Journal of Biological Chemistry 256:11905–11910
    [Google Scholar]
  2. Bause E., Legler G. 1980; Isolation and structure of a tryptic glycopeptide from the active site of /f-glucosidase A3 from Aspergillus wentii. Biochimica et Biophysica Acta 626:459–465
    [Google Scholar]
  3. Berger E., Jones W.A., Jones D.T., Woods D.R. 1989; Cloning and sequencing of an endoglucanase (endl) gene from Butyrivibrio fibrisolvens H17C. Molecular and General Genetics 219:193–198
    [Google Scholar]
  4. Bradford M.M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  5. Caldwell R.C., Bryant M.P. 1966; Medium without rumen fluid for nonselective enumeration and isolation of rumen bacteria. Applied Microbiology 14:794–801
    [Google Scholar]
  6. Cheng S.-C., Modrich P. 1983; Positive selection cloning vehicle useful for overproduction of hybrid proteins. Journal of Bacteriology 154:1005–1008
    [Google Scholar]
  7. Coughlan M.P. 1985; The properties of fungal and bacterial cellulases with comment on their production and application. Biotechnology and Genetic Engineering Review 3:39–109
    [Google Scholar]
  8. Cowing D.W., Bardwell J.C.A., Craig E.A., Woolford C., Hendrix R.W., Gross C.A. 1985; Consensus sequence for Escherichia coli heat shock gene promoters. Proceedings of the National Academy of Sciences of the United States of America 82:2679–2683
    [Google Scholar]
  9. Dagert M., Ehrlich S.D. 1979; Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene 6:23–28
    [Google Scholar]
  10. Dehority B.A. 1966; Characterization of several bovine rumen bacteria isolated with a xylan medium. Journal of Bacteriology 91:1724–1729
    [Google Scholar]
  11. Deshpande M.V., Eriksson R.-E., Pettersson L.G. 1984; An assay for selective determination of exo-1,4-β-glucanases in a mixture of cellulolytic enzymes. Analytical Biochemistry 138:481–487
    [Google Scholar]
  12. Gräbnitz F., Rucknagel K.P., Seiß M., Staundenbauer W.L. 1989; Nucleotide sequence of the Clostridium thermocellum bglB gene encoding thermostable β-glucosidase B: homology to fungal β-glucosidases. Molecular and General Genetics 217:70–76
    [Google Scholar]
  13. Hawley D.K., Mcclure W.R. 1983; Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Research 11:2237–2255
    [Google Scholar]
  14. Henikoff S. 1984; Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing. Gene 28:351–359
    [Google Scholar]
  15. Hespell R.B., Wolf R., Bothast R.J. 1987; Fermentation of xylans by Butyrivibrio fibrisolvens and other ruminal bacteria. Applied and Environmental Microbiology 53:2849–2853
    [Google Scholar]
  16. Ish-Horowicz D., Burke J.F. 1981; Rapid and efficient cosmid cloning. Nucleic Acids Research 9:2989–2998
    [Google Scholar]
  17. Kohchi C., Toh-E A. 1985; Nucleotide sequence of Candida pelliculosa β-glucosidase gene. Nucleic Acids Research 13:6273–6282
    [Google Scholar]
  18. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  19. Love D.R., Fisher R., Bergquist P.L. 1988; Sequence structure and expression of a cloned β-glucosidase gene from an extreme thermophile. Molecular and General Genetics 213:84–92
    [Google Scholar]
  20. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  21. Mannarelli B.M. 1988; Deoxyribonucleic acid relatedness among strains of the species Butyrivibrio fibrisolvens. International Journal of Systematic Bacteriology 38:340–347
    [Google Scholar]
  22. Margherita S.S., Hungate R.E. 1963; Serological analysis of Butyrivibrio from the bovine rumen. Journal of Bacteriology 86:855–860
    [Google Scholar]
  23. Messing J., Crea R., Seeburg P.H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  24. Orpin C.G., Mathiesen S.D., Greenwood Y., Blix A.S. 1985; Seasonal changes in the ruminal microflora of the high-arctic Svalbard reindeer (Rangifer tarandus plytyrhynchus). Applied and Environmental Microbiology 50:144–151
    [Google Scholar]
  25. Ostroff G.R., Péne J.J. 1983; Molecular cloning with bifunctional plasmid vectors in Bacillus subtilis: isolation of a spontaneous mutant of Bacillus subtilis with enhanced transformabi- lity for Escherichia coli-propagated chimeric plasmid DNA. Journal of Bacteriology 156:934–936
    [Google Scholar]
  26. Pardee A.B., Jacob F., Monod J. 1959; The genetic control and cytoplasmic expression of “inducibility” in the synthesis of fi- galactosidase by Escherichia coli . Journal of Molecular Biology 1:165–178
    [Google Scholar]
  27. Raynal A., Gerbaud C., Francingues M.C., Guerineau M. 1987; Sequence and transcription of the β-glucosidase gene of Kluyveromyces fragilis cloned in Saccharomyces cerevisiae . Current Genetics 12:175–184
    [Google Scholar]
  28. Rigby P.W.J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  29. Robson L.M., Chambliss G.H. 1986; Cloning of the Bacillus subtilis DLG β-l,4-glucanase gene and its expression in Escherichia coli and B. subtilis . Journal of Bacteriology 165:612–619
    [Google Scholar]
  30. Salser W. 1977; Globin mRNA sequences: analysis of base pairing and evolutionary implications. Cold Spring Harbor Symposia on Quantitative Biology 42:985–1002
    [Google Scholar]
  31. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  32. Schnetz K., Toloczyki C., Rak B. 1987; β-Glucoside (bgl) operon of Escherichia coli K-12: nucleotide sequence, genetic organization, and possible evolutionary relationship to regulatory components of two Bacillus subtilis genes. Journal of Bacteriology 169:2579–2590
    [Google Scholar]
  33. Shane B.S., Gouws L., Kistner A. 1969; Cellulolytic bacteria occurring in the rumen of sheep conditioned to low-protein teff hay. Journal of General Microbiology 55:445–457
    [Google Scholar]
  34. Smith G.E., Summers M.D. 1980; The bidirectional transfer of DNA and RNA to nitrocellulose or diazobenzyloxymethyl-paper. Analytical Biochemistry 109:123–129
    [Google Scholar]
  35. Strydom E., Mackie R.I., Woods D.R. 1986; Detection and characterization of extracellular proteases in Butyrivibrio fibrisolvens H17C. Applied Microbiology and Biotechnology 24:214–217
    [Google Scholar]
  36. Sykes R.B., Nordstrom K. 1972; Microiodometric determination of /Hactamase activity. Antimicrobial Agents and Chemotherapy 1:94–99
    [Google Scholar]
  37. Wakarchuk W.W., Greenberg N.M., Kilburn D.G., Miller R.C., Warren R.A.J. 1988; Structure and transcription analysis of the gene encoding a cellobiase from Agrobacterium sp. strain ATCC 21400. Journal of Bacteriology 170:301–307
    [Google Scholar]
  38. Willis R.C., Morris R.G., Cirakoglu C., Schellenberg G.D., Gerber N.H., Furlong C.E. 1974; Preparation of the periplasmic binding proteins from Salmonella typhimurium and Escherichia coli. . Archives of Biochemistry and Biophysics 162:64–75
    [Google Scholar]
  39. Wood W.B. 1966; Host specificity of DNA produced by Escherichia coli: bacterial mutations affecting the restriction and modification of DNA. Journal of Molecular Biology 16:118–133
    [Google Scholar]
  40. Zabeau M., Stanley K.K. 1982; Enhanced expression of cro-fi-galactosidase fusion proteins under the control of PR promoter of bacteriophage λ. EMBO Journal 1:1217–1224
    [Google Scholar]
  41. Zappe H., Jones D.T., Woods D.R. 1986; Cloning and expression of Clostridium acetobutylicum endoglucanase, cellobiase and amino acid biosynthesis genes in Escherichia coli . Journal of General Microbiology 132:1367–1372
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-8-1567
Loading
/content/journal/micro/10.1099/00221287-136-8-1567
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error