Summary: A3(2) mutants are blocked in the formation of aerial hyphae. A phage library of wildtype DNA was used to isolate recombinant phages which restore wild-type morphological development to several mutants. Of several mutations, one, , previously mapped at was not complemented by the cloned region, indicating that the locus is composed of at least two distinct genes. Partial localization of -complementing activity showed that a 1·5 kb fragment is sufficient for complementation of the mutation whereas requires the same region as well as additional sequences. Under stringent conditions, genomic DNA hybridizing to the cloned sequences was absent from other species, including the closely related 66. DNA sequences causing marked plasmid structural instability in , but not in , are also located in this region.


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