Summary: Conditions for obtaining stable protoplasts from and their reversion to hyphal growth were determined. 1,3-β-Glucan synthase activity was detected in particulate enzyme fractions from mycelium and protoplasts of . UDP-[U-C]glucose was linearly incorporated into a β-glucan for about 20 min at 25 °C. Optimum pH and temperature values, as well as thermal stabilities of the 1,3-β-glucan synthase activity, were determined. High concentrations of EDTA were inhibitory. Enzyme activity was stimulated by ATP and GTP. The apparent value for UDP-glucose was 0·54 mM. The reaction product was characterized as 1,3-β-glucan by C NMR spectroscopy and hydrolysis products of an exo-1,3-β-glucanase.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error