1887

Abstract

Conditions for obtaining stable protoplasts from and their reversion to hyphal growth were determined. 1,3--Glucan synthase activity was detected in particulate enzyme fractions from mycelium and protoplasts of . UDP-[U-C]glucose was linearly incorporated into a -glucan for about 20 min at 25 °C. Optimum pH and temperature values, as well as thermal stabilities of the 1,3--glucan synthase activity, were determined. High concentrations of EDTA were inhibitory. Enzyme activity was stimulated by ATP and GTP. The apparent value for UDP-glucose was 0·54 m. The reaction product was characterized as 1,3--glucan by C NMR spectroscopy and hydrolysis products of an exo-1,3--glucanase.

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1990-08-01
2024-04-19
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References

  1. Andaluz E., Guillén A., Larriba G. 1986; Preliminary evidence for a glucan acceptor in the yeast Candida albicans. Biochemical Journal 240:495–502
    [Google Scholar]
  2. Beauvais A., Latgé J.-P. 1989; Chitin and β(l–3)glucan synthase in the protoplastic entomophthorales. Archives of Microbiology 152:229–236
    [Google Scholar]
  3. Catley B.J. 1983; Regulation of yeast and fungal polysaccharides excluding chitin and cellulose. Progress in Industrial Microbiology 18:129–200
    [Google Scholar]
  4. Cerenius L., Söderhöll K. 1984; Isolation and properties of β- glucan synthetase from the aquatic fungus Aphanomyces astaci. Physiologia Plantarum 60:247–252
    [Google Scholar]
  5. Deshpande M.V., Balkrishnan H., Ranjekar P.K., Shankar V. 1987; Isolation and immobilization of Sclerotium rolfsii protoplasts. Biotechnology Letters 9:49–52
    [Google Scholar]
  6. Finkelman M.A.J., Vardanis A. 1987; Synthesis of β-glucan by cell-free extracts of Aureobasidium pullulans. Canadian Journal of Microbiology 33:123–127
    [Google Scholar]
  7. Girard V., Fèvre M. 1984; Distribution of (1–3)-β- and (1–4)-β-glucan synthases along the hyphae of Saprolegnia monoica. Journal of General Microbiology 130:1557–1562
    [Google Scholar]
  8. Goldstein I.J., Hay G.W., Lewis B.A., Smith F. 1965; Controlled degradation of polysaccharides by periodate oxidation, reduction and hydrolysis. Methods in Carbohydrate Chemistry 5:361–369
    [Google Scholar]
  9. Gooday G.W., Trinci A.J.P. 1980; Wall structure and biosynthesis in fungi. Symposia of the Societyfor General Microbiology 30:207–251
    [Google Scholar]
  10. Halleck F.E. 1967; Polysaccharides and methods for the production thereof. US Patent No.3 301848.
    [Google Scholar]
  11. Hamlyn P.F., Bradshaw R.E., Mellon F.M., Santiago C.M., Wilson J.M., Peberdy J.F. 1981; Efficient protoplast isolation from fungi using commercial enzymes. Enzyme and Microbial Technology 3:321–325
    [Google Scholar]
  12. Ishikawa F., Oishi K. 1985; Formation and regeneration of protoplasts from Conidiobolus lamprauges. Journal of General Microbiology 131:3311–3316
    [Google Scholar]
  13. Jacobsen T., Jensen B., Olsen J., Allermann K. 1985; Preparation of protoplasts from mycelium and arthroconidia of Geotrichum candidum. Canadian Journal of Microbiology 31:93–96
    [Google Scholar]
  14. Johnson J. JR Misaki A., Nelson T.E., Scaletti J.V., Smith F. 1963; Structure of a new glucan. Chemistry and Industry820–822
    [Google Scholar]
  15. Kang M.S., Cabib E. 1986; Regulation of fungal cell wall growth: A guanine nucleotide-binding, proteinaceous component required for activity of (l–3)-β-d-glucan synthase. Proceedings of the National Academy of Sciences of the United States of America 83:5808–5812
    [Google Scholar]
  16. Kominato M., Kamimiya S., Tanaka H. 1987; Preparation and properties of β-glucan synthase of Pyricularia oryzae P2. Agricultural and Biological Chemistry 51:755–761
    [Google Scholar]
  17. Krebs K.G., Heusser D., Wimmer H. 1967; Spriihreagentien. In Dünnschichtchromatographie pp. 813–859 Stahl E. Edited by Berlin: Springer Verlag;
    [Google Scholar]
  18. Leal F., Ruiz-Herrera J., Villanueva J.R., Larriba G. 1984; An examination of factors affecting the instability of Saccharomyces cerevisiae glucan synthetase in cell free extracts. Archives of Microbiology 137:209–214
    [Google Scholar]
  19. Lopez-Romero E., Ruiz-Herrera J. 1978; Properties of β-glucan synthetase from Saccharomyces cerevisiae. Antonie van Leeuwenhoek 44:329–339
    [Google Scholar]
  20. Orlean P.A.B. 1982; l,3-β-d-Glucan synthase from budding and filamentous cultures of the dimorphic fungus Candida albicans. European Journal of Biochemistry 127:397–403
    [Google Scholar]
  21. Quigley D.R., Selitrennikoff C.P. 1984; β(l–3)Glucan synthase activity of Neurospora crassa: stabilization and partial characterization. Experimental Mycology 8:202–214
    [Google Scholar]
  22. Rapp P. 1989; l,3-β-Glucanase, l,6-β-glucanase and β-glucosidase activities of Sclerotium glucanicum: synthesis and properties. Journal of General Microbiology 135:2847–2858
    [Google Scholar]
  23. Rapp P., Beck C.H., Wagner F. 1979; Formation of expolysaccharides by Rhodococcus erythropolis and partial characterization of a heteropolysaccharide of high molecular weight. European Journal of Applied Microbiology and Biotechnology 7:67–78
    [Google Scholar]
  24. Rinaudo M., Vlncendon M. 1982; 13C NMR structural investigation of scleroglucan. Carbohydrate Polymers 2:135–144
    [Google Scholar]
  25. Rodgers N.E. 1973; Scleroglucan. In Industrial Gums pp. 499–511 Whistler R.L., Bemiller J.N. Edited by London: Academic Press;
    [Google Scholar]
  26. San-Blas G. 1979; Biosynthesis of glucans by subcellular fractions of Paracoccidioides brasiliensis. Experimental Mycology 3:249–258
    [Google Scholar]
  27. Shematek E.M., Cabib E. 1980; Biosynthesis of the yeast cell wall. II. Regulation of β-(l-3)glucan synthetase by ATP and GTP. Journal of Biological Chemistry 255:895–902
    [Google Scholar]
  28. Shematek E.M., Braatz J.A., Cabib E. 1980; Biosynthesis of the yeast cell wall. I. Preparation and properties of β-(l-3)glucan synthetase. Journal of Biological Chemistry 255:888–894
    [Google Scholar]
  29. Somogyi M. 1952; Notes on sugar determination. Journal of Biological Chemistry 195:19–28
    [Google Scholar]
  30. Szaniszlo P.J., Kang M.S., Cabib E. 1985; Stimulation of β(l-3)glucan synthetase of various fungi by nucleoside triphosphates : generalized regulatory mechanism for cell wall biosynthesis. Journal of Bacteriology 161:1188–1194
    [Google Scholar]
  31. De vries O.M.H., Wessels J.G.H. 1972; Release of protoplasts from Schizophyllum commune by a lytic enzyme preparation from Trichoderma viride. . Journal of General Microbiology 73:13–22
    [Google Scholar]
  32. De vries O.M.H., Wessels J.G.H. 1973; Release of protoplasts from Schizophyllum commune by combined action of purified a-1,3- glucanase and chitinase derived from Trichoderma viride. Journal of General Microbiology 76:319–330
    [Google Scholar]
  33. De vries O.M.H., Wessels J.G.H. 1975; Chemical analysis of cell wall regeneration and reversion of protoplasts from Schizophyllum commune. Archives of Microbiology 102:209–218
    [Google Scholar]
  34. Wang M.C., Bartnicki-Garcia S. 1982; Synthesis of noncellulo-sic cell-wall β-glucan by cell-free extracts from zoospores and cysts of Phytophthora palmivora. Experimental Mycology 6:125–135
    [Google Scholar]
  35. Wessels J.G.H., Sietsma J.H., Sonnenberg A.S.M. 1983; Wall synthesis and assembly during hyphal morphogenesis in Schizophyllum commune. Journal of General Microbiology 129:1607–1616
    [Google Scholar]
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