1887

Abstract

Two enzymes that hydrolysed lactose were purified essentially to homogeneity from cell extracts of the oleaginous yeast . One enzyme of 120000 had properties typical of a -galactosidase (EC 3.2.1.23). It hydrolysed lactose, lactulose and nitrophenyl---galactosides. The enzyme required K or Rb for activity, and other monovalent cations tested were not effective. Enzyme activity was abolished by EDTA and stimulated by Mg, Mn and Ca. The -galactosidase was induced by lactose, galactose, lactulose and lactobionic acid. The other enzyme, a -glycosidase (EC 3.2.1.21) of 52000 showed no ionic requirements and it hydrolysed lactose, nitrophenyl---galactosides, 4-nitrophenyl---glucoside, cellobiose, laminaribiose, laminaritriose and sophorose, but not gentiobiose, 4-nitrophenyl---mannoside or sucrose. This enzyme was induced by lactose, galactose and lactulose, and also by cellobiose.

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1990-08-01
2021-05-14
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