1887

Abstract

The uptake of pyrimidines and their derivatives into and was measured using a novel technique in which the cells were rapidly separated from their suspending medium by centrifugation through a layer of an inert oil. The uptake of [C]cytosine was linear for 30 s for all concentrations of pyrimidine tested. In but not cytosine transport was mediated by both a high affinity ( 0·8 ± 0·1 μ), low capacity [ 40 ± 4 pmol(μl cell water) s] and a low affinity [ 240 ± 35 μ], high capacity system [770 ± 170 pmol (μl cell water) s. The cytosine permease in was specific for cytosine and 5-fluorocytosine. In there was only one cytosine transport system [ 2·4 ± 0·3 μ; 50 ± 4 pmol (μl cell water) s]; this system also transported adenine, guanine and hypoxanthine. Differences in nucleoside transport were also observed for and , with the uridine permease in transporting only uridine and 5-fluorouridine whereas cytidine and adenosine were also transported by the uridine permease in . Studies on the effect of nucleoside analogues on uridine transport in demonstrated the importance of the sugar moiety in determining the specificity of transport, with a hydroxyl residue on C-2 being apparently essential for transport.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-8-1475
1990-08-01
2021-05-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/8/mic-136-8-1475.html?itemId=/content/journal/micro/10.1099/00221287-136-8-1475&mimeType=html&fmt=ahah

References

  1. Arnold W.N. 1973; Volume and enzyme content of the periplasmic space in yeast. Physiological Chemistry and Physics 5:117–123
    [Google Scholar]
  2. Chevallier M.R., Jund R., Lacroute F. 1975; Characterization of cytosine permeation in Saccharomyces cerevisiae. Journal of Bacteriology 122:629–641
    [Google Scholar]
  3. Fasoli M.O.F. 1989 Pyrimidine transport and salvage metabolism in pathogenic Candida. PhD thesis University of Cambridge, UK.:
    [Google Scholar]
  4. Fasoli M.O.F., Kerridge D. 1988; Isolation and characterization of fluoropyrimidine resistant mutants in two Candida species. Annals of the New York Academy of Sciences 544:260–263
    [Google Scholar]
  5. Foret M., Schmidt R., Reichert U. 1978; On the mechanism of substrate binding to the pyrimidine transport system of Saccharomyces cerevisiae. European Journal of Biochemistry 82:33–43
    [Google Scholar]
  6. Harley E.R., Paterson A.R.P., Cass C.E. 1982; Initial rate kinetics of the transport of adenosine 4-amino-7-(β-d-ribofuranosyl) pyrrolo[2-3-d]pyridine, Tubericidin in cultured cells. Cancer Research 42:1289–1295
    [Google Scholar]
  7. Hopkins P., Chevallier M.R., Jund R., Eddy A.A. 1988; Use of plasmid vectors to show that uracil and cytosine permeases of the yeast Saccharomyces cerevisiae are electrogenic proton symports. FEMS Microbiology Letters 49:173–177
    [Google Scholar]
  8. Jund R., Chevallier M.R., Lacroute F. 1977; Uracil transport in Saccharomyces cerevisiae. Journal of Membrane Biology 36:233–251
    [Google Scholar]
  9. Lossan R., Jund R., Chevallier M.R. 1978; Properties of three distinct pyrimidine transport systems in yeast. Evidence for distinct energy coupling. Biochimica et Biophysica Acta 513:296–300
    [Google Scholar]
  10. Martindale 1982 The General Pharmacopoeia, 28th edn.. Reynolds J. E. F. Edited by London: Pharmaceutical Press;
    [Google Scholar]
  11. Polak A., Grenson M. 1973; Evidence for a common transport system for cytosine, adenine and hypoxanthine in Saccharomyces cerevisiae and Candida albicans. European Journal of Biochemistry 32:276–282
    [Google Scholar]
  12. Rao T.V.G., Verna R.S., Prasad R. 1983; Transport of purines, pyrimidine bases and nucleosides in Candida albicansa pathogenic yeast. Biochemistry International 6:409–417
    [Google Scholar]
  13. Reichert V., Foret M. 1977; Energy coupling in hypoxanthine transport in yeast.Potentiometric evidence for proton symport and potassium antiport. FEBS Letters 83:325–328
    [Google Scholar]
  14. Scherrer R., Louden L., Gerhardt P. 1974; Porosity of the yeast cell wall and membrane. Journal of Bacteriology 118:534–540
    [Google Scholar]
  15. Whelan W.L., Kerridge D. 1984; Decreased activity of UMP: pyrophosphorylase associated with resistance to 5-fluorocytosine in Candida albicans. Antimicrobial Agents and Chemotherapy 26:570–574
    [Google Scholar]
  16. Whelan W.L., Simon S., Beneke E.S., Rogers A.L. 1984; Auxotrophic mutants of Torulopsis glabrata. FEMS Microbiology Letters 24:1–4
    [Google Scholar]
  17. Wohlhueter R.M., Marz R., Graft J.C., Plagemann P.G.W. 1978; A rapid-mixing technique to measure transport in suspended animal cells. Applications to nucleoside transport in Novikoff rat hepatoma cells. Methods in Cell Biology 20:211–236
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-8-1475
Loading
/content/journal/micro/10.1099/00221287-136-8-1475
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error