1887

Abstract

Lactoferrin and transferrin have antimicrobial activity against selected Gram-negative bacteria, but the mechanism of action has not been defined. We studied the ability of lactoferrin and transferrin to damage the Gram-negative outer membrane. Lipopolysaccharide release by the proteins could be blocked by concurrent addition of Ca and Mg. Addition of Ca also blocked the ability of lactoferrin to increase the susceptibility of to rifampicin. Transferrin, but not lactoferrin, increased susceptibility of Gram-negative bacteria to deoxycholate, with reversal of sensitivity occurring with exposure to Ca or Mg. In transmission electron microscopy studies polymyxin B caused finger-like membrane projections, but no morphological alterations were seen in cells exposed to EDTA, lactoferrin or transferrin. These data provide further evidence that lactoferrin and transferrin act as membrane-active agents with the effects modulated by Ca and Mg.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1437
1990-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1437.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1437&mimeType=html&fmt=ahah

References

  1. Arnold R.R., Cole M.F., Mcghee J.R. 1977; A bactericidal role for human lactoferrin. Science 197:263–265
    [Google Scholar]
  2. Arnold R.R., Russell J.E., Champion W.J., Brewer M., Gauthier J.J. 1982; Bactericidal activity of human lactoferrin: differentiation from the stasis of iron deprivation. Infection and Immunity 35:792–797
    [Google Scholar]
  3. Boesman-Finkelstein M., Finkelstein R.A. 1985; Antimicrobial effects of human milk. FEMS Microbiology Letters 27:167–174
    [Google Scholar]
  4. Bortner C.A., Miller R.D., Arnold R.R. 1986; Bactericidal effect of lactoferrin on Legionella pneumophila. Infection and Immunity 51:373–377
    [Google Scholar]
  5. Bryan C.S. 1974; Sensitization of E. coli to the serum bactericidal system and to lysozyme by ethyleneglycoltetraacetic acid. Proceedings of the Society of Experimental Biology and Medicine 145:1431–1433
    [Google Scholar]
  6. Bullen J.J. 1981; The significance of iron in infection. Reviews of Infectious Diseases 3:1127–1138
    [Google Scholar]
  7. Bullen J.J., Rogers H.J., Griffiths E. 1978; Role of iron in bacterial infection. Current Topics in Microbiology and Immunology 80:1–35
    [Google Scholar]
  8. Coughlin R.T., Tonsager S., Mcgroaty E.J. 1983; Quantitation of metal cations bound to membranes and extracted lipopolysaccharide of Escherichia coli. Biochemistry 22:2002–2007
    [Google Scholar]
  9. Ellison R.T. III Giehl T.J., Laforce F.M. 1988; Damage of the outer membrane of enteric Gram-negative bacteria by lactoferrin and transferrin. Infection and Immunity 56:2774–2781
    [Google Scholar]
  10. Finkelstein R.A., Sciortino C.V., Mcintosh M.A. 1983; Role of iron in microbe-host interactions. Reviews of Infectious Diseases 5:S759–S777
    [Google Scholar]
  11. Fitzgerald S.P., Rogers H.J. 1980; Bacteriostatic effect of serum: role of antibody to lipopolysaccharide. Infection and Immunity 27:302–308
    [Google Scholar]
  12. Goldman R.C., Leive L. 1980; Heterogeneity of antigenic-side- chain length in lipopolysaccharide from Escherichia coli Ol11 and Salmonella typhimurium LT2. European Journal of Biochemistry 107:145–153
    [Google Scholar]
  13. Griffiths E. 1975; Effect of pH and haem compounds on the killing of Pasteurella septica by specific antiserum. Journal of General Microbiology 88:345–354
    [Google Scholar]
  14. Hancock R.E.W., Wong P.G.W. 1984; Compounds which increase the permeability of the Pseudomonas aeruginosa outer membrane. Antimicrobial Agents and Chemotherapy 26:48–52
    [Google Scholar]
  15. Hukari R., Helander I.M., Vaara M. 1986; Chain length heterogeneity of lipopolysaccharide released from Salmonella typhimurium by ethylenediaminetetraacetic acid or polycations. European Journal of Biochemistry 154:673–676
    [Google Scholar]
  16. Joiner K.A. 1985; Studies on the mechanism of bacterial resistance to complement-mediated killing and on the mechanism of bactericidal antibody. Current Topics in Microbiology and Immunology 121:99–133
    [Google Scholar]
  17. Joiner K.A., Goldman R., Schmetz M., Berger M., Hammer C.H., Frank M.M., Leive L. 1984; A quantitative analysis of C3 binding to O-antigen capsule lipopolysaccharide and outer membrane protein of E. coli 0111B4. Journal of Immunology 132:369–375
    [Google Scholar]
  18. Joiner K.A., Grossman N., Schmetz M., Leive L. 1986; C3 binds preferentially to long-chain lipopolysaccharide during alternative pathway activation by Salmonella montevideo. Journal of Immunology 136:710–715
    [Google Scholar]
  19. Kalmar J.R., Arnold R.R. 1988; Killing of Actinobacillus actinomycetemcomitans by human lactoferrin. Infection and Immunity 56:2552–2557
    [Google Scholar]
  20. Laforce F.M., Boose D.S. 1981; Sublethal damage of Escherichia coli by lung lavage. American Review of Respiratory Disease 124:733–737
    [Google Scholar]
  21. Leive L. 1974; The barrier function of the Gram-negative envelope. Annals of the New York Academy of Sciences 235:109–127
    [Google Scholar]
  22. Masson P.L., Heremans J.F., Dive C. 1966; An iron-binding protein common to many external secretions. Clinica Chimica Acta 13:735–739
    [Google Scholar]
  23. Masson P.L., Heremans J.F., Schonne E. 1969; Lactoferrin an iron-binding protein in neutrophilic leukocytes. Journal of Experimental Medicine 130:643–658
    [Google Scholar]
  24. Moguilevsky N., Retegui L.A., Masson P.L. 1985; Comparison of human lactoferrins from milk and neutrophilic leucocytes. Biochemical Journal 229:353–359
    [Google Scholar]
  25. Mokrasch L.C., Mcgilvery R.W. 1956; Purification and properties of fructose-1,6-diphosphatase. Journal of Biological Chemistry 221:909–927
    [Google Scholar]
  26. Nikaido H., Vaara M. 1985; Molecular basis of bacterial outer membrane permeability. Microbiological Reviews 49:1–32
    [Google Scholar]
  27. Norrod P., Williams R.P. 1978; Effects of iron and culture filtrates on killing of Neisseria gonorrhoeae by normal human serum. Infection and Immunity 21:918–924
    [Google Scholar]
  28. Pollack C., Straley S.C., Klempner M.S. 1986; Probing of the phagolysosomal environment of human macrophages with a Ca2+- responsive operon fusion in Yersinia pestis. Nature; London: 322834–836
    [Google Scholar]
  29. Rainard P. 1986; Bacteriostasis of Escherichia coli by bovine lactoferrin transferrin and immunoglobulins (IgGl, IgG2, IgM) acting alone or in combination. Veterinary Microbiology 11:103–115
    [Google Scholar]
  30. Redhead K., Hill T., Chart H. 1987; Interaction of lactoferrin and transferrins with the outer membrane of Bordetella pertussis. Journal of General Microbiology 133:891–898
    [Google Scholar]
  31. Spik G., Cheron A., Montreuil J., Dolby J.M. 1976; Bacteriostasis of a milk-sensitive strain of Escherichia coli by immunoglobulins and iron-binding proteins in association. Infection and Immunity 14:911–918
    [Google Scholar]
  32. Stephens S., Dolby J.M., Montreuil J., Spik G. 1980; Differences in inhibition of the growth of commensal and enteropathogenic strains of Escherichia coli by lactotransferrin and secretory immunoglobulin A isolated from human milk. Immunology 41:597–603
    [Google Scholar]
  33. Vaara M., Vaara T. 1983a; Polycations as outer membrane- disorganizing agents. Antimicrobial Agents and Chemotherapy 24:114–122
    [Google Scholar]
  34. Vaara M., Vaara T. 1983b; Sensitization of Gram-negative bacteria to antibiotics and complement by a non-toxic oligopeptide. Nature; London: 303526–528
    [Google Scholar]
  35. Vaara M., Viljanen P. 1985; Binding of polymyxin B nonapeptide to Gram-negative bacteria. Antimicrobial Agents and Chemotherapy 27:548–554
    [Google Scholar]
  36. Vaara M., Viljanen P., Vaara T., Makela P.H. 1984; An outer membrane-disorganizing peptide PMBN sensitizes E. coli strains to serum bactericidal action. Journal of Immunology 132:2582–2589
    [Google Scholar]
  37. Weiss J., Muello K., Victor M., Elsbach P. 1984; The role of lipopolysaccharides in the action of the bactericidal/permeability increasing neutrophil protein on the bacterial envelope. Journal of Immunology 132:3109–3115
    [Google Scholar]
  38. White A., Handler P., Smith E.L., Hill R.L., Lehman I.R. eds. eds 1978; Principles of Biochemistry. , 6th edn. p. 906 New York: McGraw Hill;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1437
Loading
/content/journal/micro/10.1099/00221287-136-7-1437
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error