RT Journal Article SR Electronic(1) A1 Collins, Margaret E. A1 Patki, Abhay A1 Wall, Sue A1 Nolan, Ann A1 Goodger, Jane A1 Woodward, Martin J. A1 Dale, Jeremy W.YR 1990 T1 Cloning and characterization of the gene for the “19 kD antigen of Mycobacterium bovis JF Microbiology, VO 136 IS 7 SP 1429 OP 1436 DO https://doi.org/10.1099/00221287-136-7-1429 PB Microbiology Society, SN 1465-2080, AB Monoclonal antibody CMA134.1 reacted with a protein antigen of apparent molecular mass 22 kDa from Mycobacterium bovis and Mycobacterium tuberculosis, and with an apparently 24 kDa antigen of Mycobacterium kansasii, but not with other mycobacteria or related species. This antibody was used to screen a gene library of M. bovis in λgt11 and identified a recombinant clone that expressed a protein with an apparent molecular mass of 19–20 kDa. Gene expression occurred from the lac promoter in λgt11, but used an unidentified vector promoter, possibly that of the replication primer RNA, in the final plasmid construct. The sequence of an 840 bp fragment was determined and shown to code for a product of 15 kDa. This sequence is identical to that, independently determined, of a gene from M. tuberculosis, usually referred to as the 19 kDa antigen. The reasons for the apparent size discrepancies are discussed., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-7-1429