1887

Abstract

Monoclonal antibody CMA134.1 reacted with a protein antigen of apparent molecular mass 22 kDa from and , and with an apparently 24 kDa antigen of , but not with other mycobacteria or related species. This antibody was used to screen a gene library of in gt11 and identified a recombinant clone that expressed a protein with an apparent molecular mass of 19–20 kDa. Gene expression occurred from the promoter in gt11, but used an unidentified vector promoter, possibly that of the replication primer RNA, in the final plasmid construct. The sequence of an 840 bp fragment was determined and shown to code for a product of 15 kDa. This sequence is identical to that, independently determined, of a gene from , usually referred to as the 19 kDa antigen. The reasons for the apparent size discrepancies are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1429
1990-07-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1429.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1429&mimeType=html&fmt=ahah

References

  1. Ashbridge K.R., Booth R.J., Watson J.D., Lathigra R.B. 1989; Nucleotide sequence of the 19 kDa antigen gene from Mycobacterium tuberculosis. Nucleic Acids Research 17:1249
    [Google Scholar]
  2. Baird P.N., Hall L.M.C., Coates A.R.M. 1989; Cloning and sequence analysis of the 10 kDa antigen gene of Mycobacterium tuberculosis. Journal of General Microbiology 135:931–939
    [Google Scholar]
  3. Chaparas S.D., Maloney C.J., Hendrik S.R. 1970; Specificity of tuberculins and antigens from various species of mycobacteria. American Review of Respiratory Diseases 101:74–83
    [Google Scholar]
  4. Collins M.E., Moss M.T., Wall S., Dale J.W. 1987; Expression of biotinylated proteins in mycobacterial gene libraries. FEMS Microbiology Letters 43:53–56
    [Google Scholar]
  5. Dale J.W., Patki A. 1990; Mycobacterial gene expression and regulation. In The Molecular Biology of Mycobacteria. Mcfadden J. J. Edited by London: Academic Press; (in the Press)
    [Google Scholar]
  6. Devereux J., Haeberli P., Smithies O. 1984; A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Research 12:387–395
    [Google Scholar]
  7. Fall R.R., Vagelos P.R. 1972; Acetyl coenzyme A carboxylase: molecular forms and subunit composition of biotin carboxyl carrier protein. Journal of Biological Chemistry 247:8005–8015
    [Google Scholar]
  8. Huynh T.H., Young R.A., Davis R.W. 1985; Constructing and screening cDNA libraries in lambda gtlO and lambda gtl1. In DNA Cloning, 1 pp 49–78 Glover D. N. Edited by Oxford: IRL Press;
    [Google Scholar]
  9. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Morris J.A., Thorns C.J., Woolley J. 1985; The identification of antigenic determinants on Mycobacterium bovis using monoclonal antibodies. Journal of General Microbiology 131:1825–1831
    [Google Scholar]
  12. Sanger F., Nicklen S., Coulson A.R. 1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
    [Google Scholar]
  13. Shinnick T.M. 1987; The 65-kilodalton antigen of Mycobacterium tuberculosis. Journal of Bacteriology 169:1080–1088
    [Google Scholar]
  14. Shinnick T.M., Plikaytis B.P., Hyche A.D., Van landingham R.M., Walker L.V. 1989; The Mycobacterium tuberculosis BCG-a protein has homology with the Escherichia coli GroES protein. Nucleic Acids Research 17:1254
    [Google Scholar]
  15. Staden R. 1984; Measurements of the effects that coding for a protein has on a DNA sequence and their use for finding genes. Nucleic Acids Research 12:551–567
    [Google Scholar]
  16. Stuart F.A., Manser P.A., Mcintosh F.G. 1988; Tuberculosis in imported red deer (Cervus elaphus). Veterinary Record 122:508–511
    [Google Scholar]
  17. Thole J.E.R., Keulen W.J., Kolk A.H.J., Groothuis D.G., Berwald L.G., Tiesjema R.H., Van Embden J.D.A. 1987; Characterization, sequence determination and immunogenicity of a 64-kilodalton protein of M. bovis BCG expressed in E. coli K12. Infection and Immunity 55:1466–1475
    [Google Scholar]
  18. Thorns C.J., Morris J.A. 1986; Shared epitopes between mycobacteria and other microorganisms. Research in Veterinary Science 41:275–276
    [Google Scholar]
  19. Towbin H., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  20. Wayne L.G. 1984; Mycobacterial speciation. In The Mycobacteria Part A pp 25–65 Kubica G. P., Wayne L. G. Edited by New York & Basel: Marcel Dekker;
    [Google Scholar]
  21. Wilbur W.J., Lipman D.J. 1983; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences of the United States of America 80:726–730
    [Google Scholar]
  22. Wilesmith J.W., Williams D.R. 1987; Observations on the incidence of herds with non-visible lesioned tuberculin test reactors in South West England. Epidemiology and Infection 99:173–178
    [Google Scholar]
  23. Yamaguchi R., Matsuo K., Yamazaki A., Nagai S., Terasaka K., Yamada T. 1988; Immunogenic protein MPB57 from Mycobacterium bovis BCG: molecular cloning, nucleotide sequence and expression. FEBS Letters 240:115–117
    [Google Scholar]
  24. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
  25. Young D.B., Lathigra R., Hendrix R., Sweetser D., Young R.A. 1988; Stress proteins are immune targets in leprosy and tuberculosis. Proceedings of the National Academy of Sciences of the United States of America 85:4267–4270
    [Google Scholar]
  26. Young R.A., Bloom B.R., Grosskinsky C.M., Ivanyi J., Thomas D., Davis R.W. 1985; Dissection of Mycobacterium tuberculosis antigens using recombinant DNA. Proceedings of the National Academy of Sciences of the United States of America 82:2583–2587
    [Google Scholar]
  27. Zainuddin Z., Dale J.W. 1989; Polymorphic repetitive DNA sequences in Mycobacterium tuberculosis detected with a gene probe from a Mycobacterium fortuitum plasmid. Journal of General Microbiology 135:2347–2355
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1429
Loading
/content/journal/micro/10.1099/00221287-136-7-1429
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error