1887

Abstract

grew readily on glycerol in carbon-limited chemostat culture and expressed a high carbon conversion efficiency. However, the strain of organism used (probably var. ) proved particularly sensitive to glycerol, both respiration and growth being severely impeded by any surfeit of this compound. Sensitivity was found to correlate with an exceptionally high level of expression of glycerol kinase [activities of more than 80 μmol min (mg protein) were manifest in crude cell-free extracts], coupled with low activities of methylglyoxal synthase and of glyoxylase (enzymes of the methylglyoxal bypass). It is proposed that metabolic dysfunction results from an uncontrolled gross accumulation of glycerol phosphate (and early products of its metabolism) within the cells, coupled with depletion of the intracellular phosphate pool.

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1990-07-01
2022-06-29
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References

  1. Atkinson A., Comer M.J. 1983; Thermostableglycerokinase. US Patent no.4385120 A.
    [Google Scholar]
  2. Atkinson A., Evans C.G.T., Yeo R.G. 1975; Behaviour of Bacillus stearothermophilus grown in different media. Journal of Applied Bacteriology 38:301–304
    [Google Scholar]
  3. Baird J.K., Sherwood R.F., Carr R.J.G., Atkinson A. 1976; Enzyme purification by substrate elution chromatography from procion dye-polysaccharide matrices. FEBS Letters 70:61–66
    [Google Scholar]
  4. Bergmeyer H.U. 1974 Methoden der enzymatischen Analyse, 3rd edn. 1 p. 498 Weinheim:: Verlag Chemie.;
    [Google Scholar]
  5. Calcott P.H., Postgate J.R. 1974; The effects of β-galactosidase activity and cyclic AMP on lactose accelerated death. Journal of General Microbiology 85:85–90
    [Google Scholar]
  6. Comer M.J., Bruton C.J., Atkinson T. 1979; Purification and properties of glycerolkinase from Bacillus stearothermophilus. Journal of Applied Biochemistry 1:259–270
    [Google Scholar]
  7. Cooper R.A., Anderson A. 1970; The formation and catabolism of methylglyoxal during glycolysis in Escherichia coli. FEBS tetters 11:273–276
    [Google Scholar]
  8. Epstein I., Grossowicz N. 1969; Prototrophic thermophilic Bacillus: isolation, properties and kinetics of growth. Journal of Bacteriology 99:414–417
    [Google Scholar]
  9. Evans C.G.T., Herbert D., Tempest D.W. 1970; The continuous cultivation of micro-organisms. 2. Construction of a chemostat. Methods in Microbiology 2:227–327
    [Google Scholar]
  10. Herbert D., Phipps P.J., Strange R.E. 1971; Chemical analysis of microbial cells. Methods in Microbiology 5B:209–344
    [Google Scholar]
  11. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 111680–685
    [Google Scholar]
  12. Neijssel O.M., Hueting S., Crabbendam K.J., Tempest D.W. 1975; Dual pathways of glycerol assimilation in Klebsiella aerogenes NCIB 418.Their regulation and possible functional significance. Archives of Microbiology 104:83–87
    [Google Scholar]
  13. Pennock J., Tempest D.W. 1988; Metabolic and energetic aspects of the growth of Bacillus stearothermophilus in glucose-limited and glucose-sufficient chemostat culture. Archives of Microbiology 150:452–459
    [Google Scholar]
  14. Peterson G.L. 1977; A simplification of the protein assay method of Lowry et al. which is more generally applicable. Analytical Biochemistry 83:346–356
    [Google Scholar]
  15. Postgate J.R., Hunter J.R. 1964; Accelerated death of Aerobacter aerogenes starved in the presence of growth-limiting substrates. Journal of General Microbiology 34:459–473
    [Google Scholar]
  16. Streekstra H., Teixeira De Mattos M.J., Neijssel O.M., Tempest D.W. 1987; Overflow metabolism during anaerobic growth of Klebsiella aerogenes NCTC 418 on glycerol and dihydroxy-acetone in chemostat culture. Archives of Microbiology 147:268–275
    [Google Scholar]
  17. Wieland O. 1974; Glycerol: uvmethode. In Methoden der enzymatischen Analyse, 3rd edn. 2 pp. 1448–1450 Bergmeyer H. U. Edited by Weinheim: Verlag Chemie;
    [Google Scholar]
  18. Zwaig N., Kistler W.S., Lin E.C.C. 1970; Glycerolkinase, the pacemaker for the dissimilation of glycerol in Escherichia coli. Journal of Bacteriology 102:753–759
    [Google Scholar]
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