1887

Abstract

A mutant of QM 9414 - M8 - was isolated after -irradiation. It did not form -glucosidase during growth on glycerol or glucose, but secreted -glucosidase upon growth on cellobiose. The mutant was also able to grow on cellulose and secrete -glucosidase, but with a longer lag. -Glucosidase activity could be induced in the mutant in a replacement medium by cellobiose and -methyl -glucoside. Mycelia lacking -glucosidase were able to take up both these inducers immediately, indicating the presence of a constitutive permease. The mutant produced cellulases upon growth on lactose and - after a lag - on cellulose. Mycelia of M8 pregrown on glycerol or cellobiose could be induced by sophorose to produce cellobiohydrolase I in a replacement system. The findings show that (i) cellobiose and other -linked disaccharides can be taken up by without prior hydrolysis, and (ii) that cellobiose most probably induces -glucosidase formation during growth on cellulose.

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1990-07-01
2021-09-24
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