1887

Abstract

Addition of choline (20 m) or Tween 80 (0·06%) to the culture medium of QM 9414 increased () the secretion of protein under both carbon-catabolite-repressed and -derepressed conditions, and () cellulase secretion under carbon-catabolite-derepressed conditions. In contrast, no stimulation by choline or Tween 80 was observed with the hypersecretory strain RUT C-30. In view of the obligatory role of -glycosylation in protein secretion by this fungus, an investigation was made into the effects on this process of choline and Tween 80. A membrane preparation was isolated from both strains of and used to assay enzymes involved in -glycosylation. Significant differences were observed with respect to the activity of dolichol phosphate mannose (Dol-P-Man) synthase only. Strain QM 9414, grown on media supplemented with choline or Tween 80 exhibited a two- to threefold higher activity of Dol-P-Man synthase compared to a control lacking these supplements. This stimulatory effect was observed during growth under both carbon-catabolite-repressed and -derepressed conditions. In contrast, strain RUT C-30 exhibited decreased activities of Dol-P-Man synthase when grown in media supplemented with choline. Choline had no effect on Dol-P-Man synthase , whereas Tween 80 decreased the activity. Thus the effect of Tween 80 or choline on protein secretion by may be due to a stimulation of formation and/or activity of Dol-P-Man synthase, thereby elevating the level of -glycosylation and protein secretion.

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1990-07-01
2022-01-29
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References

  1. Bowman B.J., Borgeson C.E., Bowman E.J. 1987; Composition of Neurospora crassa vacuolar membranes and comparison to endoplasmic reticulum, plasma membrane and mitochondrial membrane. Experimental Mycology 11:197–205
    [Google Scholar]
  2. Bradford M.M. 1976; A rapid and sensitive method for quantitation of microgram quantities of protein using the principle of protein dye-binding. Analytical Biochemistry 72:248–254
    [Google Scholar]
  3. Braell W.A. 1988; Two sensitive, convenient, and widely applicable assays for marker enzyme activities specific to endoplasmic reticulum. Analytical Biochemistry 170:328–334
    [Google Scholar]
  4. Enari T.M., Niku-Paavola M.L. 1987; Enzymatic cellulose hydrolysis. CRC Critical Reviews in Biotechnology 5:67–87
    [Google Scholar]
  5. Ghosh A., Alrabiai S., Ghosh B.K., Trimino-Vasquez H., Eveleigh D.E., Montenecourt B.S. 1982; Increased endoplasmic reticulum content of a mutant of Trichoderma reesei (RUT C-30) in relation to cellulase synthesis. Enzyme and Microbial Technology 4:110–113
    [Google Scholar]
  6. Ghosh A., Ghosh B.K., Trimino-Vasquez H., Eveleigh D.E., Montenecourt B.S. 1984; Cellulase secretion from a hypercellulolytic mutant of Trichoderma reesei RUT C-30. Archives of Microbiology 140:126–133
    [Google Scholar]
  7. Jensen J.W., Schutzbach J.S. 1985; Activation of dolichyl-phospho-mannose synthase by phospholipids. European Journal of Biochemistry 153:41–48
    [Google Scholar]
  8. Kammel W.P., Kubicek C.P. 1985; Absence of postsecretional modification of extracellular proteins during growth of Trichoderma reesei on crystalline cellulose. Journal of Applied Biochemistry 7:147–152
    [Google Scholar]
  9. Kruszewska J., Messner R., Kubicek C.P., Palamarczyk G. 1989; O-Glycosylation of proteins by membrane fractions of Trichoderma reesei QM 9414. Journal of General Microbiology 135:301–307
    [Google Scholar]
  10. Kubicek C.P. 1981; Release of carboxymethyl-cellulase and β-glucosidase from cell walls of Trichoderma reesei . Applied Microbiology and Biotechnology 13:226–231
    [Google Scholar]
  11. Kubicek C.P., Panda T., Schreferl-Kunar G., Messner R., Gruber F. 1987; O-linked-but not N-linked-glycosylation is necessary for secretion of endoglucanase I and II by Trichoderma reesei . Canadian Journal of Microbiology 33:698–703
    [Google Scholar]
  12. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  13. Mandels M., Andreotti R.E. 1978; Problems and challenges in the cellulose to cellulase fermentation. Process Biochemistry 13:6–13
    [Google Scholar]
  14. Merivuori H., Siegler K.M., Sands J.A., Montenecourt B.S. 1984; Regulation of cellulase biosynthesis and secretion in fungi. Biochemical Society Transactions 13:411–414
    [Google Scholar]
  15. Messner R., Kubicek C.P. 1988; Intracellular precursors of endo-β ,4-glucanases in Trichoderma reesei . FEMS Microbiology Letters 50:227–232
    [Google Scholar]
  16. Panda T., Gruber H., Kubicek C.P. 1987; Stimulation of protein secretion in Trichoderma reesei by Tween surfactants is not correlated with changes in enzyme localization or membrane fatty acid composition. FEMS Microbiology Letters 41:85–90
    [Google Scholar]
  17. Reese E.T., Maguire A. 1969; Surfactants as stimulants of enzyme production by microorganisms. Applied Microbiology 17:242–245
    [Google Scholar]
  18. Reese E.T., Maguire A. 1971; Increase in cellulase yields by addition of surfactants to cellobiose cultures of Trichoderma viride . Developments in Industrial Microbiology 12:212–224
    [Google Scholar]
  19. Schreiber G., Kolar H., Foisner R., Kubicek C.P. 1986; Choline stimulates synthesis of extracellular proteins in Trichoderma reesei QM 9414. Archives of Microbiology 144:41–47
    [Google Scholar]
  20. Tanner W., Lehle L. 1987; Protein glycosylation in yeast. Biochimica et Biophysica Acta 906:81–99
    [Google Scholar]
  21. Umesaki Y., Kawai Y., Mutai M. 1977; Effect of Tween 80 on glucosyltransferase production in Streptococcus mutans . Applied and Environmental Microbiology 34:115–119
    [Google Scholar]
  22. Wiebe M.G., Robson G.D., Trinci A.P.J. 1989; Effect of choline on the morphology, growth and phospholipid composition of Fusarium graminearum . Journal of General Microbiology 135:2155–2162
    [Google Scholar]
  23. Wittenberger C.L., Beaman A.J., Lee L.N. 1978; Tween 80 effect on glucosyltransferase synthesis by Streptococcus salivarius . Journal of Bacteriology 133:231–239
    [Google Scholar]
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