1887

Abstract

Growth of the yeast IFP 206 in methanol- and glucose-containing media led respectively to very and weakly flocculent cells. Mannans from both kinds of cells were extracted and compared. Chemical analysis and molecular mass estimation showed some differences between the mannans from very and weakly flocculent cells, especially in quantitative amino acid content. H NMR analysis showed that both kinds of mannan contained α-1,2 and -1,2 linkages. Two acetolysis conditions, combined with H NMR analysis, revealed that mannans from both kinds of cells were composed of mannose, mannobiose, mannotriose, mannotetraose and mannopentaose side-chains with the following respective structures: Man; Manα1→2Man; Manα1→2Manα1→2Man; Man1→2Manα1→2Man; Man1→2Man1→2Manα1→2Man; Manα1→2Man1→2Man1→2Manα1→2Man. Additionally the -1,2 linkages of the non-reducing terminal residues of the mannotetraose were shown to be acetolysislabile. The mannans from very flocculent cells were richer in mannopentaose than the mannans from weakly flocculent cells. According to these results, the extended conformations in the branching moieties of the mannan could be the basis of the higher degree of flocculation of the methanol-grown cells.

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1990-07-01
2024-03-29
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References

  1. Al Mahmood S., Giummelly P., Bonaly R. 1987; Structural modiFication of mannans during flocculation of Kluyveromyces bulgaricus. Applied Microbiology and Biotechnology 26:462–467
    [Google Scholar]
  2. Al Mahmood S., Giummelly P., Bonaly R., Delmotte F., Monsigny M. 1988; Kluyveromyces bulgaricus yeast lectins: isolation of iV-acetylglucosamine and galactose-speciFic lectins, their relation with flocculation. Journal of Biological Chemistry 263:3930–3934
    [Google Scholar]
  3. Bartnicki-Garcia S. 1968; Cell wall chemistry, morphogenesis and taxonomy of fungi. Annual Review of Microbiology 22:87–108
    [Google Scholar]
  4. Bonaly R., Moulki H., Touimi Benjelloun A., PierFitte M. 1971; Etudes des parois de levures du genre Rhodotorula. II. Influence des conditions de culture sur la composition chimique des parois. Biochimica et Biophysica Acta 244:484–494
    [Google Scholar]
  5. Cohen R.E., Ballou C.E. 1980; Linkage and sequence analysis of mannose-rich glycoprotein core oligosaccharides by proton nuclear magnetic resonance spectroscopy. Biochemistry 19:4345–4358
    [Google Scholar]
  6. Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. 1956; Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28:350–356
    [Google Scholar]
  7. Funayama M., Nishikawa A., Shinoda T., Suzuki M., Fukazawa Y. 1984; Antigenic relationship between Candida parapsilosis and Candida albicans serotype B. Microbiology and Immunology 28:1359–1371
    [Google Scholar]
  8. Ghuysen J.M., Tipper D.J., Birge C.H., Strominger J.L. 1965; Structure of the cell wall of Staphylococcus aureus strain Copenhagen. VI. The soluble glycopeptide and its sequential degradation by peptidases. Biochemistry 4:2245–2254
    [Google Scholar]
  9. Ghuysen J.M., Tipper D.J., Strominger J.L. 1966; Enzymes that degrade bacterial cell walls. Methods in Enzymology 8:685–699
    [Google Scholar]
  10. Gorin P.A.J., Perlin A.S. 1956; A mannan produced by Saccharomyces rouxii. Canadian Journal of Chemistry 34:1796–1803
    [Google Scholar]
  11. Gorin P.A.J., Spencer J.F.T. 1970; Proton magnetic resonance spectroscopy - an aid in identiFication and chemotaxonomy of yeasts. Advances in Applied Microbiology 13:25–89
    [Google Scholar]
  12. Gorin P.A.J., Spencer J.F.T., Bhattacharjee S.S. 1969; Structures of yeast mannans containing both α- and β-linked D-mannopyranose units. Canadian Journal of Chemistry 47:1499–1505
    [Google Scholar]
  13. Hasenclever H.F., Mitchell W.O. 1964; A study of yeast surface antigens by agglutination inhibition. Sabouraudia 3:288–300
    [Google Scholar]
  14. Kobayashi H., Shibata N., Suzuki S. 1986; Acetolysis of Pichiapastoris IFO 0948 strain mannan containing α-1,2 and β-l,2 linkages using acetolysis medium of low sulfuric acid concentration. Archives of Biochemistry and Biophysics 245:494–503
    [Google Scholar]
  15. Kocourek J., Ballou C.E. 1969; Method for Fingerprinting yeast cell wall mannans. Journal of Bacteriology 100:1175–1181
    [Google Scholar]
  16. Kogan G., Pavliak V., Masler L. 1988a; Structural studies of mannans from the cell walls of the pathogenic yeasts Candida albicans serotypes A and B and Candida parapsilosis. Carbohydrate Research 172:243–253
    [Google Scholar]
  17. Kogan G., Pavliak V., Sandula J., Masler L. 1988b; Novel structure of the cellular mannan of the pathogenic yeast Candida krusei. Carbohydrate Research 184:171–182
    [Google Scholar]
  18. Lee Y.C., Ballou C.E. 1965; Preparation of mannobiose, mannotriose and a new mannotetraose from Saccharomyces cerevisiae mannan. Biochemistry 4:257–264
    [Google Scholar]
  19. Leleu J.B., Morilhat J.P., Bonaly R. 1977; Composition chimique de la paroid’unelevure du genre Torulopsis apres culture sur methanol et ethanol. Biochemistry 16:1167–1170
    [Google Scholar]
  20. Mcclare C.W.F. 1971; An accurate and convenient organic phosphorus assay. Analytical Biochemistry 39:527–530
    [Google Scholar]
  21. Miki B.L.A., Poon N.H., James A.P., Seligy V.L. 1982; Possible mechanism for flocculation interactions governed by gene FLOl in Saccharomyces cerevisiae. Journal of Bacteriology 150:878–889
    [Google Scholar]
  22. Okubo Y., Suzuki S. 1978; Fractional precipitation of d-mannan from bakers’ yeast with concanavalin A. Carbohydrate Research 62:135–141
    [Google Scholar]
  23. Peat S., Whelan W.J., Edwards T.E. 1961; Polysaccharides of baker’s yeast. Part IV. Mannan. Journal of the Chemical Society29–34
    [Google Scholar]
  24. Power D.M., Challinor S.W. 1969; The effects of inositol- deFiciency on the chemical composition of the yeast cell wall. Journal of General Microbiology 55:169–176
    [Google Scholar]
  25. Sawardeker J.S., Stoneker J.M., Jeanes A. 1965; Quantitative determination of monosaccharides and their alditol acetates by gas chromatography. Analytical Chemistry 57:1602–1604
    [Google Scholar]
  26. Shibata N., Fukusawa S., Kobayashi H., Tojo M., Yonezu T., Ambo A., Ohkubo Y., Suzuki S. 1989; Structural analysis of phospho-d-mannan-protein complexes isolated from yeast and mold form cells of Candida albicans NIH A-207 serotype A strain. Carbohydrate Research 187:239–253
    [Google Scholar]
  27. Suzuki M., Fukazawa Y. 1982; Immunochemical characterization of Candida albicans cell wall antigens: speciFic determinant of Candida albicans serotype Amannan. Microbiology and Immunology 26:387–402
    [Google Scholar]
  28. Thieme T.R., Ballou C.E. 1970; Constancy of cell wall mannan structure of Saccharomyces cerevisiae. Biochemical and Biophysical Research Communications 39:621–625
    [Google Scholar]
  29. Trevelyan W.E., Procter D.P., Harrison J.S. 1950; Detection of sugars on paper chromatograms. Nature; London: 166:444–445
    [Google Scholar]
  30. Weill C.E., Hanke P. 1962; Thin-layer chromatography of malto-oligosaccharides. Analytical Chemistry 34:1736–1737
    [Google Scholar]
  31. Yamashita K., Mizuochi T., Kobata A. 1982; Analysis of oligosaccharides by gel Filtration. Methods in Enzymology 83:105–126
    [Google Scholar]
  32. Zhang W.J., Ballou C.E. 1981; Saccharomyces kluyveri cell wall mannoprotein.Structures of the O- and N-linked carbohydrate components. Journal of Biological Chemistry 256:10073–10079
    [Google Scholar]
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