We have identified an insertion sequence, IS116, present in Streptomyces clavuligerus at one copy per genome. The element was discovered as a 1·4 kb insertion into the multicopy plasmid pIJ702 after propagation in S. clavuligerus. The nucleotide sequence of IS116 and the flanking sequences from pIJ702 have been determined. The junctions with pIJ702 show no target site duplication and there are no inverted repeats at the ends of the element. One putative coding open reading frame of 1197 bp was identified which would code for a protein product of 399 amino acids. This protein resembles deduced integrase/transposase proteins specified by three other transposable elements of actinomycetes: IS110 and the mini-circle from Streptomyces coelicolor A3(2), and - most particularly - IS900 of Mycobacterium paratuberculosis. Two regions that are relatively conserved among these gene products show features found in similar positions in many reverse transcriptases. IS116 and IS900 are also closely similar in their general organization and (apparently) in their insertion site specificity, whereas IS110 and the mini-circle are quite different in these features.
BaileyC.R.,
WinstanleyD.J.1986; Inhibition of restriction in Streptomyces clavuligerus by heat treatment. Journal of General Microbiology 132:2945–2947
BibbM.J.,
FindlayP.R.,
JohnsonM.W.1984; The relationship between base composition and codon usage in bacterial genes and its use in the simple and reliable identification of protein coding sequences. Gene 30:157–166
ChaterK.F.,
BrutonC.J.,
FosterS.G.,
TobekI.1985; Physical and genetic analysis of IS110 a transposable element of Streptomyces coelicolor A3(2). Molecular and General Genetics 200:235–239
ChaterK.F.,
HendersonD.J.,
BibbM.J.,
HopwoodD.A.1988; Genome flux in Streptomyces coelicolor and other streptomyces and its possible relevance to the evolution of mobile antibiotic resistance determinants. In Transposition, pp 7–42KingsmanA. J.,
ChaterK. F.,
KingsmanS. M.
Edited by Cambridge: Cambridge University Press;
GreenE.P.,
TizardM.L.V.,
MossM.T.,
ThompsonJ.,
WinterbourneD.J.,
McFaddenJ.J.,
Hermon-TaylorJ.1989; Sequence and characteristics of IS900, an insertion element identified in a human Crohn’s disease isolate of Mycobacterium paratuberculosis
. Nucleic Acids Research 17:9063–9073
GringauzE.,
OrleK.A.,
WaddellC.S.,
CraigN.L.1988; Recognition of Escherichia coli attTn7 by transposon Tn7: lack of specific sequence requirements at the point of Tn7 insertion. Journal of Bacteriology 170:2832–2840
HendersonD.J.,
LydiateD.J.,
HopwoodD.A.1989; Structural and functional analysis of the mini-circle, a transposable element of Streptomyces coelicolor A3(2). Molecular Microbiology 3:1307–1318
InouyeS.,
HsuM.-Y.,
EagleS.,
InouyeM.1989; Reverse transcriptase associated with the biosynthesis of the branched RNA-linked msDNA in Myxococcus xanthus
. Cell 56:709–717
JensenS.E.,
WestlakeD.W.S.,
WolfeS.1982; Cyclization of δ-(l-α-aminoadipyl)-l-cysteinyl-d-valine to penicillins by cell free extracts of Streptomyces clavuligerus
. Journal of Antibiotics 35:483–490
JensenS.E.,
LeskiwB.K.,
ViningL.C.,
AharonowitzY.,
WestlakeD.W.S.,
WolfeS.1986; Purification of isopenicillin N synthetase from Streptomyces clavuligerus
. Canadian Journal of Microbiology 32:953–958
JohnsonM.S.,
McClureM.A.,
FengD.-F.,
GrayJ.,
DoolittleR.F.1986; Computer analysis of retroviralpol genes: assignment of enzymatic function to specific sequences and homologies with non-viral enzymes. Proceedings of the National Academy of Sciences of the United States of America 83:7648–7652
KatzE.,
ThompsonC.J.,
HopwoodD.A.1983; Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans
. Journal of General Microbiology 129:2703–2714
KendallK.J.,
CohenS.N.1988; Complete nucleotide sequence of the Streptomyces lividans plasmid pIJ101 and correlation of the sequence with genetic properties. Journal of Bacteriology 170:4634–4651
LampsonB.C.,
SunJ.,
HsuM.-Y.,
Vallejo-RamirezJ.,
InouyeS.,
InouyeM.1989; Reverse transcriptase in a clinical strain of Escherichia coli: production of branched RNA-linked msDNA. Science 243:1033–1038
LeskiwB.K.,
AharonowitzY.,
MevarechM.,
WolfeS.,
ViningL.C.,
WestlakeD.W.S.,
JensenS.E.1988; Cloning and nucleotide sequence determination of the isopenicillin N synthetase gene from Streptomyces clavuligerus
. Gene 62:187–196
LydiateD.J.,
IkedaH.,
HopwoodD.A.1986; A 2·6 kb DNA sequence of Streptomyces coelicolor A3(2) which functions as a transposable element. Molecular and General Genetics 203:79–88
LydiateD.J.,
AshbyA.M.,
HendersonD.J.,
KieserH.M.,
HopwoodD.A.1989; Physical and genetic characterization of linear copies of the Streptomyces coelicolor mini-circle. Journal of General Microbiology 135:941–955
MillsD.R.,
KramerF.R.1979; Structure-independent nucleotide sequence analysis. Proceedings of the National Academy of Sciences of the United States of America 76:2232–2235
MizusawaS.,
NishimuraS.,
SeelaF.1986; Improvement of the dideoxy chain termination method of DNA sequencing by use of deoxy-7-deazaguanosine triphosphate in place of dGTP. Nucleic Acids Research 14:1319–1324
MurphyE.1988; Transposable elements in Staphylococcus
. In Transposition, pp 59–89KingsmanA. J.,
ChaterK. F.,
KingsmanS. M.
Edited by Cambridge: Cambridge University Press;
SangerF.,
NicklenS.,
CoulsonA.R.1977; DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
XiongY.,
EickbushT.H.1988; Similarity of reverse transcriptase-like sequences of viruses, transposable elements, and mitochondrial introns. Molecular Biology of Evolution 5:675–690