1887

Abstract

PAO lacks UV-inducible Weigle reactivation and Weigle mutagenesis of UV-damaged bacteriophages. This lack of UV-inducible, error-prone DNA repair appears to be due to the absence of efficiently expressed -like genes in this species. When the gene is introduced into a (Def) mutant of K12, the gene product is capable of mediating UV-induced mutagenesis, indicating that it could participate in a -like regulatory network and function in inducible DNA repair pathways if such existed in . The presence of the IncP9, UV-resistance plasmid R2 in RecA strains of PAO allows UV-inducible, mutagenic DNA repair of UV-irradiated bacteriophages. R2 also greatly stimulates the ability of UV radiation to induce mutagenesis of the bacterial chromosome. When R2 is introduced into strains containing either the or mutation, plasmid-mediated UV resistance and Weigle reactivation are not observed. These observations suggest that the increased protection afforded to by R2 is derived from a RecA-mediated, DNA-damage-inducible, error-prone DNA repair system which complements the lack of a chromosomally encoded -like operon.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1241
1990-07-01
2022-01-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1241.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1241&mimeType=html&fmt=ahah

References

  1. Bale M.J., Fry J.C., Day M.J. 1988; Transfer and occurrence of large mercury resistance plasmids in river epilithon. Applied and Environmental Microbiology 54:972–978
    [Google Scholar]
  2. Calkins J., Buckles J.D., Moeller J.R. 1976; The role of solar UV radiation in ‘natural’ water purification. Photochemistry and Photobiology 24:49–57
    [Google Scholar]
  3. Cavenagh M.M., Miller R.V. 1986; Specialized transduction of Pseudomonas aeruginosa PAO by bacteriophage D3. Journal of Bacteriology 165:448–452
    [Google Scholar]
  4. Darzins A., Chakrabarty A.M. 1984; Cloning of genes controlling alginate biosynthesis from a mucoid cystic fibrosis isolate of Pseudomonas aeruginosa . Journal of Bacteriology 159:9–18
    [Google Scholar]
  5. Egan J.B., Holloway B.W. 1961; Genetic studies on lysogeny in Pseudomonas aeruginosa . Australian Journal of Experimental Biology 39:9–18
    [Google Scholar]
  6. Elledge S.J., Walker G.C. 1983; The rriuc genes of pKM101 are induced by DNA damage. Journal of Bacteriology 155:1306–1315
    [Google Scholar]
  7. Horn J.M., Ohman D.E. 1988; Transcriptional and translational analysis of recA mutant alleles in Pseudomonas aeruginosa . Journal of Bacteriology 170:1937–1950
    [Google Scholar]
  8. Jacoby G.A. 1977; Classification of plasmids in Pseudomonas aeruginosa . In Microbiology 1977 pp 119–126 Schlessinger D. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  9. Jacoby G.A., Sutton L., Knobel L., Mammen P. 1983; Properties of IncP-2 plasmids of Pseudomonas spp. Antimicrobial Agents and Chemotherapy 24:168–175
    [Google Scholar]
  10. Kato T., Shinoura Y. 1977; Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light. Molecular and General Genetics 156:121–131
    [Google Scholar]
  11. Kato T., Rothman R.H., Clark A.J. 1977; Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation. Genetics 87:1–18
    [Google Scholar]
  12. Kokjohn T.A., Miller R.V. 1985; Molecular cloning and characterization of the recA gene of Pseudomonas aeruginosa PAO. Journal of Bacteriology 163:568–572
    [Google Scholar]
  13. Kokjohn T.A., Miller R.V. 1987; Characterization of the Pseudomonas aeruginosa recA analogue and its protein product: rec- 102 is a mutant allele of the P. aeruginosa PAO recA gene. Journal of Bacteriology 169:1499–1508
    [Google Scholar]
  14. Kokjohn T.A., Miller R.V. 1988; Characterization of the Pseudomonas aeruginosa recA gene: the Les phenotype. Journal of Bacteriology 170:578–582
    [Google Scholar]
  15. Korfhagen T.R., Sutton L., Jacoby G.A. 1978; Classification and physical properties of Pseudomonas plasmids. In Microbiology 1978 pp 221–224 Schlessinger D. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Krishnapillai V. 1975; Resistance to ultraviolet light and enhanced mutagenesis conferred by Pseudomonas aeruginosa plasmids. Mutation Research 29:363–372
    [Google Scholar]
  17. Kung A.H.C., Less B.T.O. 1973; The isolation and characterization of radiation and chemical mutagen sensitive mutants of Pseudomonas aeruginosa . Mutation Research 20:175–190
    [Google Scholar]
  18. Langer P.J., Shanabruch W.G., Walker G.C. 1981; Functional organization of plasmid pKM 101. Journal of Bacteriology 145:1310–1316
    [Google Scholar]
  19. Lehrbach P.R., Kung A.H.C., Lee B.T.O. 1978; R plasmids which alter ultraviolet light-sensitivity and enhance ultraviolet light- induced mutability in Pseudomonas aeruginosa . Journal of General Microbiology 108:119–123
    [Google Scholar]
  20. Lehrbach P.R., Lee B.T.O., Dirckze C.D. 1979; Effect of repair deficiency and R plasmids on spontaneous and radiation- induced mutability in Pseudomonas aeruginosa . Journal of Bacteriology 139:953–960
    [Google Scholar]
  21. Little J.W., Mount D.W. 1982; The SOS regulatory system of Escherichia coli . Cell 29:11–22
    [Google Scholar]
  22. Little J.W., Edmiston S.H., Pacelli L.Z., Mount D.W. 1980; Cleavage of the Escherichia coli lex A protein by the recA protease. Proceedings of the National Academy of Sciences of the United States of America 77:3225–3229
    [Google Scholar]
  23. Mcbeth D.L. 1989; Effect of degradative plasmid CAM-OCT on responses of Pseudomonas bacteria to UV light. Journal of Bacteriology 171:975–982
    [Google Scholar]
  24. Miller R.V., Kokjohn T.A. 1988; Expression of the recA gene of Pseudomonas aeruginosa PAO is inducible by DNA-damaging agents. Journal of Bacteriology 170:2385–2387
    [Google Scholar]
  25. Miller R.V., Ku C.-M.C. 1978; Characterization of Pseudomonas aeruginosa mutants deficient in the establishment of lysogeny. Journal of Bacteriology 134:875–883
    [Google Scholar]
  26. Nohmi T., Battista J.R., Dodson L.A., Walker G.C. 1988; RecA-mediated cleavage activates UrauD for mutagenesis: mechanistic relationship between transcriptional derepression and posttranslational activation. Proceedings of the National Academy of Sciences of the United States of America 85:1816–1820
    [Google Scholar]
  27. O’Morchoe S.M., Ogunseitan O., Sayler G.S., Miller R.V. 1988; Conjugal transfer of R68.45 and FP5 between Pseudomonas aeruginosa strains in a freshwater environment. Applied and Environmental Microbiology 54:1923–1929
    [Google Scholar]
  28. Peak M.J., Peak J.G. 1989; Solar-ultraviolet-induced damage to DNA. Photodermatology 6:1–15
    [Google Scholar]
  29. Saye D.J., Ogunseitan O., Sayler G.S., Miller R.V. 1987; Potential for transduction of plasmids in a natural freshwater environment: effect of donor concentration and a natural microbial community on transduction in Pseudomonas aeruginosa . Applied and Environmental Microbiology 53:987–995
    [Google Scholar]
  30. Sedgwick S.G., Goodwin P.A. 1985; Differences in mutagenic and recombinational repair in enterobacteria. Proceedings of the National Academy of Sciences of the United States of America 82:4172–4176
    [Google Scholar]
  31. Smith C.M., Eisenstadt E. 1989; Identification of a umuDC locus in Salmonella typhimurium LT2. Journal of Bacteriology 171:3860–3865
    [Google Scholar]
  32. Thomas S.M., Sedgwick S.G. 1989; Cloning of Salmonella typhimurium DNA encoding mutagenic DNA repair. Journal of Bacteriology 171:5776–5782
    [Google Scholar]
  33. Walker G.C. 1984; Mutagenesis and inducible responses to deoxyribonucleic acid damage in Escherichia coli . Microbiological Reviews 48:60–93
    [Google Scholar]
  34. Weigle J.J. 1953; Induction of mutation in a bacterial virus. Proceedings of the National Academy of Sciences of the United States of America 39:628–636
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1241
Loading
/content/journal/micro/10.1099/00221287-136-7-1241
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error