Glycine-induced cryotransformation of plasmids into Free

Abstract

Different cloning vectors (pC194, pBC16, pUB110, pBD10, pBD8, pAMβ1) and plasmid pX02 were introduced into by a transformation method. To induce an artificial competence state for uptake of isolated plasmid DNA, the cultures were treated with glycine, to reduce cross-linking of peptidoglycan, followed by freezing and thawing. The procedure is extremely rapid and relatively efficient (maximum transformation efficiency about 10 c.f.u. per μg DNA) and allows different cloning vectors with molecular masses ranging from 1·8 to 17·7 MDa to be introduced into .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1217
1990-07-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1217.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1217&mimeType=html&fmt=ahah

References

  1. Bartkus J.M., Leppla S.H. 1989; Transcriptional regulation of the protective antigen gene of Bacillus anthracis. Infection and Immunity 57:2295–2300
    [Google Scholar]
  2. Battisti L., Green B.D., Thorne C.B. 1985; Mating system for transfer of plasmids among Bacillus anthracis, Bacillus cereus, and Bacillus thuringiensis. Journal of Bacteriology 162:543–550
    [Google Scholar]
  3. Bernhard K., Schrempf H., Goebel W. 1978; Bacteriocin and antibiotic resistance plasmids in Bacillus cereus and Bacillus subtilis. Journal of Bacteriology 133:897–903
    [Google Scholar]
  4. Clewell D.B., Yagi Y., Dunny G., Schultz S. 1974; Characterization of three plasmid DNA molecules in a strain of Streptococcus faecalis. Identification of a plasmid determining erythromycin resistance. Journal of Bacteriology 117:283–289
    [Google Scholar]
  5. Dityatkin S.YA., Lisovskaya K.V., Panzava N.N., Ilya-Shenko B.N. 1972; Frozen-thawed bacteria as recipients of isolated coliphage DNA. Biochimica et Biophysica Acta 281:319–323
    [Google Scholar]
  6. Dityatkin S.Ya, Loginova O.G., Kim A.A., Ilyashenko B.N. 1985; Cryotransformation and cryotransfection of bacteria. Cryo Letters 6:67–72
    [Google Scholar]
  7. Ehrlich S.D. 1977; Replication and expression, of plasmids from Staphylococcus aureus in Bacillus subtilis. Proceedings of the National Academy of Sciences of the United States of America 74:1680–1682
    [Google Scholar]
  8. Green B.D., Battisti L., Koehler T.M., Thorne C.B., Ivins B.E. 1985; Demonstration of capsule plasmid in Bacillus anthracis. Infection and Immunity 49:291–297
    [Google Scholar]
  9. Green B.D., Battisti L., Thorne C.B. 1989; Involvement of Tn4430 in transfer of Bacillus anthracis plasmids mediated by Bacillus thuringiensis plasmid pX012. Journal of Bacteriology 171:104–113
    [Google Scholar]
  10. Gryczan T.J., Contente S., Dubnau D. 1978; Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. Journal of Bacteriology 134:318–329
    [Google Scholar]
  11. Hammes W., Schleifer K.N., Kandler O. 1973; Mode of action of glycine on biosynthesis of peptidoglycan. Journal of Bacteriology 116:1029–1053
    [Google Scholar]
  12. Heierson A., Landen R., Lövgren A., Dalhammar G., Boman H.G. 1987; Transformation of vegetative cells of Bacillus thuringiensis by plasmid DNA. Journal of Bacteriology 169:1147–1152
    [Google Scholar]
  13. Holsters M., De Waele D., Depieker A., Messens E., Van montagu M., Schell J. 1978; Transfection and transformation of Agrobacterium tumefaciens. Molecular and General Genetics 163:181–187
    [Google Scholar]
  14. Ivins B., Welkos S.L. 1986; Cloning and expression of the Bacillus anthracis protective antigen in Bacillus subtilis. Infection and Immunity 54:537–542
    [Google Scholar]
  15. Koehler T.M., Thorne C.B. 1987; Bacillus subtilis (natto) plasmid pLS20 mediate interspecies plasmid transfer. Journal of Bacteriology 169:5271–5278
    [Google Scholar]
  16. Kokoushkhin A.M. 1982; Transformation of plague microbe with DNA of homologous plasmids. In Molecular Biology and Genetics of Particularly Dangerous Infectious Pathogens, part 2 pp. 34–40 Sratov, USSR: All-Union Antiplague Research Institute ‘Microbe’; (in Russian)
    [Google Scholar]
  17. Loginova O.G., Dityatkin S.YA. 1988; Cryotransformation of Bacillus cereus by plasmid DNA. Molecular Genetics, Microbiology and Virology 11:44–48
    [Google Scholar]
  18. Makino S., Sasakawa C., Uchida I., Terakado N., Yoshikava M. 1987; Transformation of a cloning vector, pUBl 10, into Bacillus anthracis. FEMS Microbiology Letters 44:45–48
    [Google Scholar]
  19. Makino S., Sasakava O., Uchida S., Terakado N., Yoshikawa M. 1988; Cloning and C02-dependent expression of the genetic region for encapsulation from Bacillus anthracis. Molecular Microbiology 2:371–376
    [Google Scholar]
  20. Merrick M.J., Gibbins J.R., Postgate J.R. 1987; A rapid and efficient method for the plasmid transformation of Klebsiella pneumoniae and Escherichia coli. Journal of General Microbiology 133:2053–2057
    [Google Scholar]
  21. Mozharov O.T. 1982; Optimization of extraction of homologous plague microbe plasmids. In Molecular Biology and Genetics of Particularly Dangerous Infectious Pathogens, part 1 pp. 48–52 Sratov, USSR: All-Union Antiplague Research Institute ‘Microbe’; (in Russian)
    [Google Scholar]
  22. Reddy A., Battisti L., Thorne C.B. 1987; Identification of selftransmissible plasmids in four Bacillus thuringiensis subspecies. Journal of Bacteriology 169:5263–5270
    [Google Scholar]
  23. Robertson D.L., Leppla S.H. 1986; Molecular cloning and expression in Escherichia coli of the lethal factor gene of Bacillus anthracis. Gene 44:71–78
    [Google Scholar]
  24. Ruhfel R.E., Robillard N.J., Thorne C.B. 1984; Interspecies transduction of plasmids among Bacillus anthracis, B. cereus, and B. thuringiensis. Journal of Bacteriology 157:708–711
    [Google Scholar]
  25. Stepanov A.S., Gavrilov S.V., Puzanova O.B., Grigoryeva T.M., Azizbekyan R.R. 1989; Transduction of plasmids in Bacillus anthracis by bacteriophage CP54ant. Molecular Genetics, Microbiology and Virology 1:14–19
    [Google Scholar]
  26. Takahashi W., Yamagata H., Yamaguchi K., Tskukagoshi N., Udaka S. 1983; Genetic transformation of Bacillus brevis 47 a protein secreting bacterium, by plasmid DNA. Journal of Bacteriology 156:1130–1134
    [Google Scholar]
  27. Thome C.B. 1978; Transduction in Bacillus thuringiensis. Applied and Environmental Microbiology 35:1109–1115
    [Google Scholar]
  28. Thorne C.B. 1985; Genetics of Bacillus anthracis. In Microbiology - 1985 pp. 56–62 Leive L. Edited by Washington DC: American Society for Microbiology;
    [Google Scholar]
  29. Tippets M.T., Robertson D.L. 1988; Molecular cloning and expression of Bacillus anthracis edema factor toxin gene: a calmodulin-dependent adenylate cyclase. Journal of Bacteriology 170:2263–2266
    [Google Scholar]
  30. Vodkin M.H., Leppla S.H. 1983; Cloning of protective antigen gene of Bacillus anthracis. Cell 34:693–697
    [Google Scholar]
  31. Weiss A.A., Falkow S. 1982; Plasmid transfer to Bordetella pertussis: conjugation and transformation. Journal of Bacteriology 152:549–552
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1217
Loading
/content/journal/micro/10.1099/00221287-136-7-1217
Loading

Data & Media loading...

Most cited Most Cited RSS feed