RT Journal Article SR Electronic(1) A1 Stepanov, S. A1 Puzanova, Olga B. A1 Dityatkin, Stanislav Ya. A1 Loginova, Olga G. A1 Ilyashenko, Boris N.YR 1990 T1 Glycine-induced cryotransformation of plasmids into Bacillus anthracis JF Microbiology, VO 136 IS 7 SP 1217 OP 1221 DO https://doi.org/10.1099/00221287-136-7-1217 PB Microbiology Society, SN 1465-2080, AB Different cloning vectors (pC194, pBC16, pUB110, pBD10, pBD8, pAMβ1) and Bacillus anthracis plasmid pX02 were introduced into B. anthracis by a transformation method. To induce an artificial competence state for uptake of isolated plasmid DNA, the cultures were treated with glycine, to reduce cross-linking of peptidoglycan, followed by freezing and thawing. The procedure is extremely rapid and relatively efficient (maximum transformation efficiency about 103 c.f.u. per μg DNA) and allows different cloning vectors with molecular masses ranging from 1·8 to 17·7 MDa to be introduced into B. anthracis., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-7-1217