1887

Abstract

Different cloning vectors (pC194, pBC16, pUB110, pBD10, pBD8, pAMβ1) and plasmid pX02 were introduced into by a transformation method. To induce an artificial competence state for uptake of isolated plasmid DNA, the cultures were treated with glycine, to reduce cross-linking of peptidoglycan, followed by freezing and thawing. The procedure is extremely rapid and relatively efficient (maximum transformation efficiency about 10 c.f.u. per μg DNA) and allows different cloning vectors with molecular masses ranging from 1·8 to 17·7 MDa to be introduced into .

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1990-07-01
2022-01-28
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