1887

Abstract

A collection of 117 strains of , including 112 non-typable isolates recovered predominantly in the USA and France from genital, obstetric and neonatal sources, was characterized by the electrophoretic mobilities of 10 metabolic enzymes. Eighty-six distinctive multilocus chromosomal genotypes (electrophoretic types, ETs) were distinguished on the basis of allele profiles at the enzyme loci. Isolates of five allied biotype IV ETs were highly divergent from all other strains and hybridization of chromosomal DNA revealed that they undoubtedly represent a previously unrecognized species of . Isolates representing these ETs were recovered predominantly from obstetric infections and serious neonatal diseases and apparently possess specific tropism for the genital tract. Strains of these five ETs were present in samples from both the USA and France, but only in the USA did they cause bacteraemia and meningitis, an occurrence which probably reflects differences in patient management between the two countries. Although strains assigned to () were strongly polymorphic in multilocus enzyme genotype, 69% of isolates recovered from patients with meningitis and/or septicaemia were assigned to only two clone families, a result suggesting that some serologically non-typable strains of originating from the genital tract are unusually virulent.

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1990-07-01
2021-10-27
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References

  1. Brenner D.J. 1984; Family 1.EnterobacteriaceaeRahn 1937. In Bergey’s Manual of Systematic Bacteriology, 1 pp 408–420 Krieg N. R., Holt J. G. Edited by Baltimore: Williams & Wilkins;
    [Google Scholar]
  2. Brenner D.J., Mcwhorter A.C., Leete Knutson J.K., Steigerwalt A.G. 1982; Escherichia vulneris: a new species of Enterobacteriaceae associated with human wounds. Journal of Clinical Microbiology 15:1133–1140
    [Google Scholar]
  3. Campognone P., Singer D.B. 1986; Neonatal sepsis due to nontypable Haemophilus influenzae. American Journal of Diseases of Children 140:117–121
    [Google Scholar]
  4. Chun P.K., Sensabaugh G.F., Vedros N.A. 1985; Genetic relationships among Neisseria species assessed by comparative enzyme electrophoresis. Journal of General Microbiology 131:3105–3115
    [Google Scholar]
  5. Crosa J.H., Brenner D.J., Falkow S. 1973; Use of a singlestrand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. Journal of Bacteriology 115:904–911
    [Google Scholar]
  6. Friesen C.A., Cho C.T. 1986; Characteristic features of neonatal sepsis due to Haemophilus influenzae. Reviews of Infectious Diseases 8:777–780
    [Google Scholar]
  7. Gilmour M.N., Whittam T.S., Kilian M., Selander R.K. 1987; Genetic relationships among the oral streptococci. Journal of Bacteriology 169:5247–5257
    [Google Scholar]
  8. Goullet PH., Picard B. 1986; Highly pathogenic strains of Escherichia coli revealed by the distinct electrophoretic patterns of carboxylesterase B. Journal of General Microbiology 132:1853–1858
    [Google Scholar]
  9. Holmes R.L., De Franco M., Otto M. 1982; Novel method of biotypingHaemophilus influenzae that uses API 20. Journal of Clinical Microbiology 15:1150–1152
    [Google Scholar]
  10. Kilian M. 1976; A taxonomic study of the genus Haemophilus, with the proposal of a new species. Journal of General Microbiology 93:9–62
    [Google Scholar]
  11. Kilian M. 1980; Haemophilus. In Manual of Clinical Microbiology, 3rd edn. pp 330–336 Lennette E. H., Balows A., Hausler W. J. JR Truant J. P. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  12. Kleiman M.B., Reynolds J.K., Shreiner R.L., Smith J.W. 1983; Failure to demonstrate special virulence of nontypableHaemophilus influenzae biotype 4 in neonatal sepsis. Journal of Infectious Diseases 148:615
    [Google Scholar]
  13. Mendelman P.M., Chaffin D.O., Musser J.M., De Groot R., Serfass D.A., Selander R.K. 1987; Genetic and phenotypic diversity among ampicillin-resistant, non-β-lactamase-producing, nontypeableHaemophilus influenzae isolates. Infection and Immunity 55:2585–2589
    [Google Scholar]
  14. Musser J.M., Granoff D.M., Pattison P.E., Selander R.K. 1985; A population genetic framework for the study of invasive diseases caused by serotype b Haemophilus influenzae. Proceedings of the National Academy of Sciences of the United States of America 82:5078–5082
    [Google Scholar]
  15. Musser J.M., Barenkamp S.J., Granoff D.M., Selander R.K. 1986a; Genetic relationships of serologically nontypable and serotype b strains of Haemophilus influenzae. Infection and Immunity 52:183–191
    [Google Scholar]
  16. Musser J.M., Hewlett E.L., Peppler M.S., Selander R.K. 1986b; Genetic diversity and relationships in populations of Bordetella spp. Journal of Bacteriology 166:230–237
    [Google Scholar]
  17. Musser J.M., Bemis D.A., Ishikawa H., Selander R.K. 1987; Clonal diversity and host distribution in Bordetellabronchiseptica. Journal of Bacteriology 169:2793–2803
    [Google Scholar]
  18. Musser J.M., Kroll J.S., Moxon E.R., Selander R.K. 1988a; Clonal population structure of encapsulated Haemophilus influenzae. Infection and Immunity 56:1837–1845
    [Google Scholar]
  19. Musser J.M., Kroll J.S., Moxon E.R., Selander R.K. 1988b; Evolutionary genetics of encapsulated strains of Haemophilus influenzae. Proceedings of the National Academy of Sciences of the United States of America 85:7758–7762
    [Google Scholar]
  20. Ochman H., Whittam T.S., Caugant D.A., Selander R.K. 1983; Enzyme polymorphism and genetic population structure in Escherichia coli and Shigella. Journal of General Microbiology 129:2715–2726
    [Google Scholar]
  21. Popoff M., Coynault C. 1980; Use of DEAE-cellulose filters in the SI nuclease method for bacterial desoxyribonucleic acid hybridization. Annales de l’Institut Pasteur/Microbiologie 131A:151–155
    [Google Scholar]
  22. Quentin R., Goudeau A., Burfin E., Pinon G., Berger CH., Laugier J., Soutoul J.H. 1987; Infections materno-foetales a Haemophilus influenzae. La Presse Médicate 16:1181–1184
    [Google Scholar]
  23. Quentin R., Koubaa N., Cattier B., Gavignet M., Goudeau A. 1988; In vitro activities of five new quinolones against 88 genital and neonatal Haemophilus isolates. Antimicrobial Agents and Chemotherapy 32:147–149
    [Google Scholar]
  24. Quentin R., Musser J.M, Sizaret P.Y., Selander R.K., Goudeau A. 1989; Typing of urogenital, maternal and neonatal isolates of Haemophilus influenzae and Haemophilusparainfluenzae in correlation with clinical source of isolation and evidence for a genital specificity of H. influenzae biotype IV. Journal of Clinical Microbiology 27:2286–2294
    [Google Scholar]
  25. Selander R.K., Musser J.M. 1990; The population genetics of bacterial pathogenesis. In Molecular Basis of Bacterial Pathogenesis. Iglewski B. H., Clark V. L. Edited by Orlando: Academic Press (in the Press);
    [Google Scholar]
  26. Selander R.K., Mckinney R.M., Whittam T.S., Bibb W.F., Brenner D.J., Nolte F.S., Pattison P.E. 1985; Genetic structure of populations of Legionella pneumophila. Journal of Bacteriology 163:1021–1037
    [Google Scholar]
  27. Selander R.K., Caugant D.A., Ochman H., Musser J.M., Gilmour M.N., Whittam T.S. 1986; Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Applied and Environmental Microbiology 51:873–884
    [Google Scholar]
  28. Wallace R.J. JR Baker C.J., Quinones F.J., Hollis D.G., Weaver R.E., Wiss K. 1983; NontypableHaemophilusinfluenzae (biotype 4) as a neonatal, maternal, and genital pathogen. Reviews of Infectious Diseases 5:123–136
    [Google Scholar]
  29. Wayne L.G., Brenner D.J., Colwell R.R., Grimont P.A.D., Kandler O., Krichevsky M.I., Moore L.H., Moore W.E.C., Murray R.G.T., Stackebrandt E., Starr M.P. 1987; Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. International Journal of Systematic Bacteriology 37:463–465
    [Google Scholar]
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