1887

Abstract

Techniques are described which allow mutated populations of to be enriched efficiently (up to 167-fold in one round of enrichment) for mutants deficient in the alkane degradation pathway (Alk). Such mutants, as well as being of scientific importance in studies of the degradation pathway, are also of commercial interest because several of the degradative intermediates are of value to the chemical industry. The Alk mutants were readily isolated by their inability to grow on agar plates supplied with hexadecane as sole carbon source. A total of 288 Alk mutants were isolated from, effectively, 4 x 10 mutagen-treated cells. They were further characterized by replica-plating using palmitic acid (PA) or acetate (Ac) as sole carbon source. Preliminary screening studies showed that of the 84 Alk PA Ac mutants, most could accumulate dicarboxylic acids from hexadecane and palmitic acid and at least one mutant also produced 3-hydroxyhexadecanedioic acid. Of the 80 mutants characterized as Alk PA, 16 produced small amounts of hexadecanol.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-7-1197
1990-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/7/mic-136-7-1197.html?itemId=/content/journal/micro/10.1099/00221287-136-7-1197&mimeType=html&fmt=ahah

References

  1. Akabori S., Shiio I., Uchio R. 1972; Japanese Patent 1300455.
    [Google Scholar]
  2. Bassel J., Mortimer R. 1973; Genetic analysis of mating type and alkane utilisation in Saccharomycopsis lipolytica. Journal of Bacteriology 114:894–896
    [Google Scholar]
  3. Bassel J., Ogrydziak D.M. 1979; Genetics of Saccharomycopsis lipolytica with emphasis on genetics of hydrocarbon utilisation. In Genetics of Industrial Microorganisms pp. 160–165 Sebek O.K., Laskin A.I. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  4. Boulton C.A., Ratledge C. 1984; The physiology of hydrocarbon-utilising microorganisms. In Introduction to Topics in Enzyme and Fermentation Biotechnology 9 pp. 11–77 Wiseman A. Edited by Chichester: Ellis Horwood;
    [Google Scholar]
  5. Chen S.L., Cooper E.J., Gutman S. 1966; Active dry yeast: protection against oxidative deterioration during storage. Food Technology 20:1585–1608
    [Google Scholar]
  6. Fish N.M., Allenby D.J., Lilly M.D. 1982; Oxidation of n-alkanes: growth of Pseudomonas putida. European Journal of Applied Microbiology and Biotechnology 14:259–262
    [Google Scholar]
  7. Furukawa T., Matsuyoshi T., Kise S. 1986; Selection of high brassylic acid producing strains of Torulopsis Candida by single-cell cloning and by mutation. Journal of Fermentation Technology 64:97–101
    [Google Scholar]
  8. Hill F.F., Venn I., Lukas K.L. 1986; Studies on the formation of long-chain dicarboxylic acids from pure n-alkanes by a mutant of Candida tropicalis. Applied Microbiology and Biotechnology 24:168–174
    [Google Scholar]
  9. Megnet R. 1965; Screening of auxotrophic mutants of Schizosaccharomyces pombe with 2-deoxyglucose. Mutation Research 2:328–331
    [Google Scholar]
  10. Moat A.G., Peters N., Srb A. 1959; Selection and isolation of auxotrophic yeast mutants with the aid of antibiotics. Journal of Bacteriology 77:675–677
    [Google Scholar]
  11. Ogata K., Kaneyuki H., Kato N., Tani Y., Yamada H. 1973; Accumulation of decanedioic acid from n-decane by Torulopsis Candida no. 99. Journal of Fermentation Technology 51:227–235
    [Google Scholar]
  12. Rehm H.J., Reiff J. 1981; Mechanisms and occurrence of microbial oxidation of long-chain alkanes. Advances in Biochemical Engineering 19:175–215
    [Google Scholar]
  13. Snow R. 1966; An enrichment method for auxotrophic yeast mutants using the antibiotic nystatin. Nature; London: 211206–207
    [Google Scholar]
  14. Spencer J.F.T., Spencer D.M., Tulloch A.P. 1979; Extracellular glycolipids of yeast. Economic Microbiology 3:523–540
    [Google Scholar]
  15. Taoka A. 1986; Production of brassylic acid by fermentation. Bio Industry 3:867–874
    [Google Scholar]
  16. Uchio R., Shiio I. 1972a; Microbial production of long-chain dicarboxylic acids from n-alkanes. Part II. Production by Candida cloacae mutant unable to assimilate n-alkane. Agricultural and Biological Chemistry 36:1169–1175
    [Google Scholar]
  17. Uchio R., Shiio I. 1972b; Tetradecane 1,14-dicarboxylic acid production from n-hexadecane by Candida cloacae. Agricultural and Biological Chemistry 36:1389–1397
    [Google Scholar]
  18. Uemura N. 1985; Industrialisation of the production of dibasic acids from paraffins using microorganisms. Hakko to Kogyo 43:436–441
    [Google Scholar]
  19. Yano K., Kanamuri M., Takagi M. 1981; Enrichment of n-alkane assimilation deficient mutants of Candida yeast by synergistic effect of nystatin and pyrrolnitrin. Agricultural arid Biological Chemistry 45:1017–1018
    [Google Scholar]
  20. Yi Z.H., Rehm H.J. 1982a; Metabolic formation of dodecane-dioic acid from n-dodecane by a mutant of Candida tropicalis. European Journal of Applied Microbiology and Biotechnology 14:254–258
    [Google Scholar]
  21. Yi Z.H., Rehm H.J. 1982b; A new metabolic pathway from n-dodecane to α, ω-dodecanioic acid in a mutant of Candida tropicalis. European Journal of Applied Microbiology and Biotechnology 15:175–179
    [Google Scholar]
  22. Yi Z.H., Rehm H.J. 1982; c. Formation of α, ω-dodecanedioic acid and α, ω-tridecanedioic acid from different substrates by immobilised cells of a mutant of Candida tropicalis. European Journal of Applied Microbiology and Biotechnology 16:1–4
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1197
Loading
/content/journal/micro/10.1099/00221287-136-7-1197
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error