1887

Abstract

Summary: Incubation of the plant pathogenic bacterium pv. with either -[3,4-C]methionine or [3,4-C]KMBA (2-keto-4-methylthiobutyric acid) led to the production of [C]MTPA (3-methylthiopropionic acid) and [C]MTAA (3-methylthioacrylic acid). When an excess of non-radioactive KMBA was present in the medium, formation of [C]KMBA from -[3,4-C]methionine was observed. Conversely, [C]methionine accumulated in a trapping pool of non-radioactive methionine as a result of biotransformation of [3,4-C]KMBA. Aminooxyacetic acid, a transaminase inhibitor, suppressed totally the formation of [C]MTPA from -[3,4-C]methionine but not from [3,4-C]KMBA. Metabolism of -[1-C]methionine liberated CO but did not produce [C]MTPA. These results demonstrate that methionine is catabolized by pv. into MTPA via transamination and subsequent decarboxylation of the intermediate α-keto acid KMBA.

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/content/journal/micro/10.1099/00221287-136-7-1185
1990-07-01
2019-10-23
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-7-1185
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