1887

Abstract

Incubation of the plant pathogenic bacterium pv. with either -[3,4-C]methionine or [3,4-C]KMBA (2-keto-4-methylthiobutyric acid) led to the production of [C]MTPA (3-methylthiopropionic acid) and [C]MTAA (3-methylthioacrylic acid). When an excess of non-radioactive KMBA was present in the medium, formation of [C]KMBA from -[3,4-C]methionine was observed. Conversely, [C]methionine accumulated in a trapping pool of non-radioactive methionine as a result of biotransformation of [3,4-C]KMBA. Aminooxyacetic acid, a transaminase inhibitor, suppressed totally the formation of [C]MTPA from -[3,4-C]methionine but not from [3,4-C]KMBA. Metabolism of -[1-C]methionine liberated CO but did not produce [C]MTPA. These results demonstrate that methionine is catabolized by pv. into MTPA via transamination and subsequent decarboxylation of the intermediate a-keto acid KMBA.

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1990-07-01
2022-01-22
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References

  1. Arima K., Yamashita T., Hosoda J., Tamura G. 1970; Studies on substances active on the behaviour of planarian. I. Production of rrafls-3-methylthioacrylic acid and 3-methylthiopropionic acid by microorganisms. Agricultural and Biological Chemistry 34:1178–1183
    [Google Scholar]
  2. Berthet J.A., Bondar G. 1915; Molestia bacteriana da mandioca. Boletim de Agricultura São Paulo 16:513–524
    [Google Scholar]
  3. Case G.L., Benevenga N.J. 1976; Evidence for S-adenosylmeth-ionine independent catabolism of methionine in the rat. Journal of Nutrition 106:1721–1736
    [Google Scholar]
  4. Dixon J.L., Benevenga N.J. 1980; The decarboxylation of α-keto-γ-methiolbutyrate in rat liver mitochondria. Biochemical and Biophysical Research Communications 97:939–946
    [Google Scholar]
  5. Dye D.W. 1962; The inadequacy of the usual determinative test for the identification of Xanthomonas spp. New Zealand Journal of Science 5:393–416
    [Google Scholar]
  6. Ewbank E., Maraite H. 1990; Amino acid catabolism in Xanthomonas campestris pathogenesis. Proceedings of the VIIth International Conference on Plant Pathogenic Bacteria, Budapest 11-16 June 1989 (in the Press).
    [Google Scholar]
  7. Holmberg B. 1945; Zur Kenntnis der Propiothetine. Archiv für Kemi, Mineralogi Och Geologi, BD 21:7
    [Google Scholar]
  8. Ince J.E., Knowles C.J. 1985; Ethylene formation by cultures of Escherichia coli. . Archives of Microbiology 141:209–213
    [Google Scholar]
  9. Ince J.E., Knowles C.J. 1986; Ethylene formation by cell-free extracts of Escherichia coli. . Archives of Microbiology 146:151–158
    [Google Scholar]
  10. Maraite H., Meyer J.A. 1975; Xanthomonas manihotis (Arthaud-Berthet) Starr, causal agent of bacterial wilt, blight and leaf spots of cassava in Zaïre. Pest Articles and News Summaries 21:27–37
    [Google Scholar]
  11. Mayaudon J. 1971; Use of radiorespirometry in soil microbiology and biochemistry. In Soil Biochemistry, 2202–256 Douglas Mclaren A., Skuyns J. Edited by New York: Marcel Dekker;
    [Google Scholar]
  12. Mitchell A.D., Benevenga N.J. 1978; The role of transamination in methionine oxidation in the rat. Journal of Nutrition 108:67–68
    [Google Scholar]
  13. Noda T., Soto Z., Kobayashi H., Iwazakis S., Okuda S. 1980; Isolation and structural elucidation of phytotoxic substances produced by Xanthomonas campestris pv. oryzae. . Annals of the Phytopathological Society of Japan 46:663–666
    [Google Scholar]
  14. Perreaux D., Maraite H., Meyer J.A. 1982; Identification of 3-methylthiopropionic acid as a blight-inducing toxin produced by Xanthomonas campestris pv. manihotis. . Physiological Plant Pathology 20:313–319
    [Google Scholar]
  15. Perreaux D., Maraite H., Meyer J.A. 1986; Detection of 3-methylthiopropionic acid in cassava leaves infected by Xanthomonas campestris pv. manihotis. . Physiological and Molecular Plant Pathology 28:323–328
    [Google Scholar]
  16. Robeson D.J., Cook D.R. 1985; Production of low molecular weight carboxylic acids by Xanthomonas campestris pv. campestris in relation to the amino acid composition of the medium and their possible involvement in pathogenesis. Physiological Plant Pathology 26:219–230
    [Google Scholar]
  17. Rognstad R., Clark D.G. 1974; Effects of aminooxyacetate on the metabolism of isolated liver cells. Archives of Biochemistry and Biophysics 161:638–646
    [Google Scholar]
  18. Spackman D.H., Moore S., Stein W.H. 1958; Automatic recording apparatus for use in the chromatography of amino acids. Analytical Chemistry 30:1190–1206
    [Google Scholar]
  19. Steele R.D., Benevenga N.J. 1978; Identification of 3-methylthiopropionic acid as an intermediate in mammalian methionine metabolism in vitro. . Journal of Biological Chemistry 253:7844–7850
    [Google Scholar]
  20. Stickland L.H. 1951; Determination of small quantities of bacteria by means of the biuret reaction. Journal of General Microbiology 5:698–703
    [Google Scholar]
  21. Swanson B.T., Wilkins H.F., Kennedy B.W. 1979; Factors affecting ethylene production by some plant pathogenic bacteria. Plant and Soil 51:19–26
    [Google Scholar]
  22. Watanabe T., Sekizana Y., Oda M. 1967; Biochemical studies of the host-parasite relationship between Xanthomonas oryzae and rice plant. I. Sulphur containing amino acid as an essential growth factor of the bacterium. Annals of the Phytopathological Society of Japan 30:1–7
    [Google Scholar]
  23. Young J.M., Dye D.W., Bradbury J.F., Panagopoulos C.G., Robbs C.F. 1978; A proposed nomenclature and classification for plant pathogenic bacteria. New Zealand Journal of Agricultural Research 21:153–177
    [Google Scholar]
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