1887

Abstract

A gene which is unique to the QpRS plasmid from chronic isolates of was cloned, sequenced, and expressed in This gene, termed codes for a putative surface protein of approximately 55 kDa, termed the E′ protein. The gene is 1485 bp in length, and is preceded by predicted promoter regulatory sequences of TTTAAT (−35), TATAAT (−10), and a Shine-Dalgarno sequence of GGAGAGA, all of which closely resemble those of and other rickettsiae. The open reading frame (ORF) of ends with a UAA codon followed by a second in-frame UAG stop codon and a region of dyad symmetry which may act as a rhofactor-independent terminator. The ORF of is capable of coding for a polypeptide of 495 amino acids with a predicted molecular mass of 55893 Da. The E′ protein has a predicted pI of ~8·7, and contains a distinct hydrophobic region of 12 amino acid residues. transcription/translation and expression of recombinant plasmids containing produce a protein of approximately 55 kDa. The expression of yields a novel protein that can be detected on immunoblots developed with rabbit antiserum generated against purified outer membrane from DNA hybridization analysis shows that is unique to the QpRS plasmid found in chronic isolates of , and is absent in chromosomal DNA and plasmids (QpH1, QpDG) from other isolates of A search of various DNA and amino acid sequence data bases revealed no homologies to

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1990-06-01
2021-05-09
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