1887

Abstract

The ability of to protect mice against intestinal pathology caused by toxinogenic was studied. Different regions of the intestine of experimental mice were prepared for observation by scanning electron microscopy or homogenized for enumeration and quantification of toxin A by enzyme immunoassay and toxin B by cytotoxicity. The test group was treated for 6 d with an suspension in drinking water and challenged with on day 4. The three control groups were: axenic mice, mice treated with only and mice only challenged with The results showed that: (i) 70% of the mice infected by survived when treated with ; (ii) the -induced lesions on the small and large intestinal mucosa were absent or markedly less severe in -treated mice; and (iii) there was no decrease in the number of but rather a reduction in the amount of toxins A and B in -treated mice.

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1990-06-01
2024-12-07
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References

  1. Arnon S.S., Mills D.C., Day P.A., Henrickson R.V., Sullivan N.M., Wilkins T.D. 1984; Rapid death of infant rhesus monkeys injected with Clostridium difficile toxins A and B: physiologic and pathologic basis. Journal of Pediatrics 104:34–40
    [Google Scholar]
  2. Banno Y., Kobayashi T., Kono H., Watanabe K., Ueno K., Nozawa T. 1984; Biochemical characterization and biologic actions of two toxins (D-l and D-2) from Clostridium difficile. Reviews of Infectious Diseases 6:S11–S20
    [Google Scholar]
  3. Bartlett J.G. 1986; Clostridium difficile: pseudomembranous colitis and antibotic-associated diarrhea. In Infectious Diarrhea pp. 157–178 Gorbach S.L. Edited by Boston: Blackwell Scientific Publications;
    [Google Scholar]
  4. Bartlett J.G., Chang T.W., Gurwith M., Gorbach S.L., Onderdonck A.B. 1978; Antibiotic-associated pseudomembranous colitis due to toxin-producing Clostridia. New England Journal of Medicine 298:531–536
    [Google Scholar]
  5. Castex F., Jouvert S., Bastide M. 1987; Visualization by scanning electron microscopy of the intestinal transit of Saccharo- myces boulardii in mice. Bulletin de la Société Française de Mycologie Médicate 16:249–256
    [Google Scholar]
  6. Castex F., Jouvert S., Bastide M. 1988; Kinetics of elimination of Saccharomyces boulardii in the mouse ileum: visualization by scanning electron microscopy. Revista Iberica de Micologia 5: (suppl.), 28 (Abstract)
    [Google Scholar]
  7. Corthier G., Dubos F., Raibaud P. 1985; Modulation of cytotoxin production by Clostridium difficile in the intestinal tracts of gnotobiotic mice inoculated with various human intestinal bacteria. Applied and Environmental Microbiology 49:250–252
    [Google Scholar]
  8. Corthier G., Dubos F., Ducluzeau R. 1986; Prevention of Clostridium difficile-induced mortality in gnotobiotic mice by Saccharomyces boulardii. Canadian Journal of Microbiology 32:894–896
    [Google Scholar]
  9. Corthier G., Muller M.C., Elmer G.W., Lucas F., Dubos-Ramaré F. 1989; Interrelationships between digestive proteolytic activities and production and quantitation of toxins in pseudomembranous colitis induced by Clostridium difficile in gnotobiotic mice. Infection and Immunity 57:3922–3927
    [Google Scholar]
  10. Czuprynski C.J., Johnson W.J., Balish E. 1983; Pseudomembranous colitis in Clostridium difficile monoassociated rats. Infection and Immunity 39:1368–1376
    [Google Scholar]
  11. Elmer G.W., Mcfarland L.V. 1987; Saccharomyces boulardii suppression of overgrowth of toxigenic Clostridium difficile following vancomycin treatment in the hamster. Antimicrobial Agents and Chemotherapy 31:129–131
    [Google Scholar]
  12. Libby J.M., Jortner B.S., Wilkins T.D. 1982; Effect of two toxins of Clostridium difficile in antibotic-associated cecitis in hamsters. Infection and Immunity 36:822–829
    [Google Scholar]
  13. Lima A.A.M., Lyerly D.M., Wilkins T.D., Innes D.J., Guerrant R.L. 1988; Effects of Clostridium difficile toxins A and B in rabbit small and large intestine in vivo and on cultured cells in vitro. Infection and Immunity 56:582–588
    [Google Scholar]
  14. Lonnroth I., Lange S. 1983; Toxin A of Clostridium difficile '. production, purification and effect in mouse intestine. Acta Pathologica et Microbiologica Scandinavica 91:395–400
    [Google Scholar]
  15. Lyerly D.M., Wilkins T.D. 1984; Characteristics of the toxins of Clostridium difficile. . In Antibiotic-associated Diarrhea and Colitis pp. 89–102 Borriello S.P. Edited by Boston: Martinus Nijhoff;
    [Google Scholar]
  16. Lyerly D.M., Saum K.E., Macdonald D.K., Wilkins T.D. 1985; Effects of Clostridium difficile toxins given intragastrically to animals. Infection and Immunity 47:349–352
    [Google Scholar]
  17. Lyerly D.M., Phelps C.J., Toth J., Wilkins T.D. 1986; Characterization of toxin A and B of Clostridium difficile with monoclonal antibodies. Infection and Immunity 54:70–76
    [Google Scholar]
  18. Mahé S., Corthier G., Dubos F. 1987; Effect of various diets on toxin production by two strains of Clostridium difficile in gnotobiotic mice. Infection and Immunity 55:1801–1805
    [Google Scholar]
  19. Massot J., Sanchez O., Couchy R., Astoin J., Parodi A.L. 1984; Bacterio-pharmacological activity of Saccharomyces boulardii in clindamycin-induced colitis in the hamster. ArzneimittelForschung 34:794–797
    [Google Scholar]
  20. Meador J., Tweten R.K. 1988; Purification and characterization of toxin B from Clostridium difficile. Infection and Immunity 56:1708–1714
    [Google Scholar]
  21. Mitchell T.J., Ketley J.M., Haslam S.C., Stephen J., Burdon D.W., Candy D.C.A., Daniel R. 1986; Effect of toxins A and B of Clostridium difficile on rabbit ileum and colon. Gut 27:78–85
    [Google Scholar]
  22. Sullivan N.M., Pellett S., Wilkins T.D. 1982; Purification and characterization of toxins A and B of Clostridium difficile. Infection and Immunity 35:1032–1040
    [Google Scholar]
  23. Toothaker R.D., Elmer G.W. 1984; Prevention of Clindamycin-induced mortality in hamster by Saccharomyces boulardii. Antimicrobial Agents and Chemotherapy 26:552–556
    [Google Scholar]
  24. Vernet A., Corthier G., Dubos-Ramare F., Parodi A.L. 1989; Relationship between levels of Clostridium difficile toxin A and toxin B and cecal lesions in gnotobiotic mice. Infection and Immunity 57:2123–2127
    [Google Scholar]
  25. Wilson K.H., Sheagren J.N., Freter R., Weatherbee L., Lyerly D. 1986; Gnotobiotic models for study of the microbial ecology of Clostridium difficile and Escherichia coli. Journal of Infectious Diseases 153:547–551
    [Google Scholar]
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