RT Journal Article SR Electronic(1) A1 Gromkova, Rosa A1 Koornhof, HendrikYR 1990 T1 Naturally occurring NAD-independent Haemophilus parainfluenzae JF Microbiology, VO 136 IS 6 SP 1031 OP 1035 DO https://doi.org/10.1099/00221287-136-6-1031 PB Microbiology Society, SN 1465-2080, AB Four, NAD-independent, clinical isolates of Haemophilus parainfluenzae were recovered from a genital ulcer, a purulent skin lesion, a sputum specimen and a throat swab respectively. With the exception of NAD requirement, the strains exhibited the biochemical characteristics of H. parainfluenzae biotype II. The genetic relationship between these isolates and a standard strain of H. parainfluenzae was determined by testing transforming activities of two chromosomal markers, streptomycin resistance and nalidixic acid resistance. The clinical isolates were efficient donors and recipients in transformation. In addition, we demonstrated transfer of the genes conferring NAD independence to typical, NAD-requiring H. parainfluenzae and Haemophilus influenzae strains., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-6-1031