1887

Abstract

The profile of generation and characteristics of splenic macrophages (MΦs) which suppress the concanavalin A (Con A) mitogenic response of splenic T cells (designated as ‘immunosuppressive MΦ’) in host CBA/JN mice during the course of complex (MAC) infection were investigated. In MAC-infected mice, reductions in some cellular functions of host splenic T cells, such as the Con A mitogenic response and mixed leucocyte reaction, were seen around 2 weeks after challenge of organisms, and this was accompanied by appearance of immunosuppressive MΦs in spleen cells. In this case, increase in immunosuppressive MΦ activity was seen in terms of both activity per spleen and activity per individual MΦ. In this phase of the infection, MAC-induced splenic MΦs showed a markedly increased ability to produce reactive oxygen radicals in response to phorbol myristate acetate. Thus, the expression of suppressor activity of MAC-induced MΦs seems to be closely linked to their activated state. A large proportion of the immunosuppressive MΦs exhibited suppressor activity dependent on prostaglandins and membrane functions related to microfilaments. It was also found that the generation of IL-2-reactive T cell populations in response to Con A was markedly inhibited by MAC-induced splenic MΦs, whereas they caused no significant reduction in the IL-2-producing ability of normal spleen cells.

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/content/journal/micro/10.1099/00221287-136-5-965
1990-05-01
2021-10-23
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