1887

Abstract

Water-extracted proteins from nine geographically diverse strains of , all of which agglutinated rabbit erythrocytes and rainbow trout spermatozoa, were compared by SDS-PAGE. Extracts from eight strains, including the type strain, ATCC 33209, were similar, containing a major protein of 57 kDa and a minor protein of 58 kDa. The SDS-PAGE protein profile of the Char strain did not contain the 58 kDa protein. A non-agglutinating strain, MT-239, which was also non-hydrophobic, did not produce any water-extractable protein. Immunoblot reactions with rabbit antiserum prepared against whole cells of the type strain demonstrated that the water-extracted haemagglutinins from the various strains were antigenically related. When purified by polyacrylamide gel zone electrophoresis, the haemagglutinin from ATCC 33209 formed a doublet band with molecular masses of 57 and 58 kDa, similar to the previously described F antigen. The water-extracted haemagglutinin agglutinated salmonid spermatozoa, was degraded by protease K and trypsin, and was shown to self-assemble onto the cell surface.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-5-949
1990-05-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/5/mic-136-5-949.html?itemId=/content/journal/micro/10.1099/00221287-136-5-949&mimeType=html&fmt=ahah

References

  1. Bruno D.W. 1988; The relationship between auto-aggregation, cell surface hydrophobicity and virulence of the fish pathogen Renibacterium salmoninarum . FEMS Microbiology Letters 51:135–140
    [Google Scholar]
  2. Bullock G.L., Stuckey H.W., Chen P.K. 1974; Corynebacter ial kidney disease of salmonids: growth and serological studies on the causative bacterium. Applied Microbiology 28:811–814
    [Google Scholar]
  3. Burnette W.N. 1981; ‘Western blotting’: electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  4. Daly J.G., Stevenson R.M.W. 1985; Charcoal agar, a new growth medium for the fish disease bacterium Renibacterium salmoninarum. Applied and Environmental Microbiology 50:868–871
    [Google Scholar]
  5. Daly J.G., Stevenson R.M.W. 1987; Hydrophobic and haemagglutinating properties of Renibacterium salmoninarum. Journal of General Microbiology 133:3575–3580
    [Google Scholar]
  6. Daly J.G., Stevenson R.M.W. 1989; Agglutination of salmonid spermatozoa by Renibacterium salmoninarum. Journal of Aquatic Animal Health 1:163–164
    [Google Scholar]
  7. Fryer J.L., Sanders J.E. 1981; Bacterial kidney disease of salmonid fish. Annual Review of Microbiology 35:273–298
    [Google Scholar]
  8. Getchell R.G., Rohovec J.S., Fryer J.L. 1985; Comparison of Renibacterium salmoninarum isolates by antigenic analysis. Fish Pathology 20:149–159
    [Google Scholar]
  9. Kimura T., Yoshimizu M. 1981; A coagglutination test with antibody sensitized staphylococci for rapid and simple diagnosis of bacterial kidney disease (BKD). Developments in Biological Standardization 49:135–148
    [Google Scholar]
  10. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  11. Pascho R.J., Mulcahy D. 1987; Enzyme-linked immunosorbent assay for a soluble antigen of Renibacterium salmoninarum, the causative agent of salmonid bacterial kidney disease. Canadian Journal of Fisheries and Aquatic Sciences 44:183–191
    [Google Scholar]
  12. Sanders J.E., Fryer J.L. 1980; Renibacterium salmoninarum gen. nov., sp. nov., the causative agent of bacterial kidney disease in salmonid fishes. International Journal of Systematic Bacteriology 30:496–502
    [Google Scholar]
  13. Towbin M., Staehelin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  14. Turaga P., Weins G., Kaattari S. 1987; Bacterial kidney disease: the potential role of soluble protein antigen(s). Journal of Fish Biology 31: (supplement A) 191–194
    [Google Scholar]
  15. Weins G.D., Kaattari S.L. 1989; Monoclonal antibody analysis of common surface protein(s) of Renibacterium salmoninarum . Fish Pathology 24:1–7
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-5-949
Loading
/content/journal/micro/10.1099/00221287-136-5-949
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error