Surface structures, haemagglutination and cell surface hydrophobicity of strains Free

Abstract

Nineteen strains of were examined by negative staining for surface structures. One strain (ATCC 23745) possessed peritrichous fibrils, 16 strains carried peritrichous fimbriae and two strains carried no surface structures. The fimbriae had a diameter of 2·1±0·25 nm and appeared to be ‘curly’. Only a small proportion (4 to 41%, depending on the strain) of cells in a population carried fimbriae or fibrils. Strain A312 showed phase variation of fimbriae as expression of fimbriae was repressed at 20 °C and in early exponential phase at 37 °C. The fibrils on strain ATCC 23745 did not exhibit phase variation in response to changes in incubation temperature, growth phase or growth in two different media. Capsules were demonstrated by the Indian ink method on 18 of the 19 strains, varying in size from strain to strain and within the same population. Cultures often contained both capsulate and noncapsulate cells. All strains possessed an electron dense ruthenium red staining layer between 7·9 and 23·9 nm in width attached to the outer membrane. Cell surface hydrophobicity quantified by the hexadecane partition assay gave low values ranging from 6·6 to 52·1%. Only a few strains were able to haemagglutinate and these were only weakly active. There was no correlation between cell surface hydrophobicity, haemagglutinating activity and surface structures.

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1990-05-01
2024-03-29
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References

  1. VanAlphen L., van denBerghe N., van denBroek L.G. 1988; Interaction of Haemophilus influenzae with human erythrocytes and oropharyngeal epithelial cells is mediated by a common fimbrial epitope. Infection and Immunity 56:1800–1806
    [Google Scholar]
  2. Babb J.L., Cummins C.S. 1978; Encapsulation of Bacteroidesspecies. Infection and Immunity 19:1088–1091
    [Google Scholar]
  3. Boedeker E.C., Cheney P.A., Schad P.A., Formal S.B. 1979; Hydrophobic bacterial properties correlates with the adherence of enteropathogens to intestinal brush border membranes (BBM). Gastroenterology 76:1105
    [Google Scholar]
  4. Brook I., Coolbaugh J.L., Walker R.I. 1984; Pathogenicity of piliated and encapsulated Bacteroides fragilis. . European Journal of Clinical Microbiology 3:207–209
    [Google Scholar]
  5. Cantey J.R., Luslibaugh W.S., Inman L.R. 1981; Attachment of bacteria to intestinal epithelial cells in diarrhoea caused by E. coliRDE C1 in the rabbit: stages and role of capsule. Journal of Infectious Disease 143:219–230
    [Google Scholar]
  6. Connolly J.C., McClean C., Tabaqchili S. 1984; The effect of capsular polysaccharide and lipopolysaccharide of Bacteroides fragilis on polymorph function and serum killing. Journal of Medical Microbiology 17:259–271
    [Google Scholar]
  7. Devine D.A., Gmür R., Handley P.S. 1989; Ultrastructure, serogrouping and localization of surface antigens of Bacteroides intermedius. . Journal of General Microbiology 135:967–997
    [Google Scholar]
  8. van Doorn J., Mooi F.R., Verweij-Van Vught A., MacClaren D.M. 1987; Characterization of fimbriae from Bacteroides fragilis. . Microbial Pathogenesis 3:87–95
    [Google Scholar]
  9. Duguid J.P., Old D.C. 1980; Adhesive properties of Enterobacteriaceae. . In Bacterial Adherence Series Receptors and Recognition, 6 series B, pp 187–215 Beachey E. H. Edited by London: Chapman and Hall;
    [Google Scholar]
  10. Handley P.S., Tipler L.S. 1986; An electron microscope survey of the surface structures and hydrophobicity of oral and non-oral species of the bacterial genus Bacteroides. . Archives of Oral Biology 31:325–335
    [Google Scholar]
  11. Handley P.S., Carter P.L., Fielding J. 1984; Streptococcus salivarius strains carry either fibrils or fimbriae on the cell surface. Journal of Bacteriology 157:64–72
    [Google Scholar]
  12. Handley P.S., Carter P.L., Wyatt J.E., Hesketh L.M. 1985; Surface structures (peritrichous fibrils and tufts of fibrils) found on Streptococcus sanguis strains may be related to their ability to coaggregate with other oral genera. Infection and Immunity 47:217–227
    [Google Scholar]
  13. Handley P.S., Harty D.W.S., Wyatt J.E., Brown C.R., Doran J.P., Gibbs A.C.C. 1987; A comparison of the adhesion, coaggregation and cell surface hydrophobic properties of fibrillar and fimbriate strains of Streptococcus salivarius. . Journal of General Microbiology 133:3207–3217
    [Google Scholar]
  14. Handley P.S., Hargreaves J., Harty D.W.S. 1988; Ruthenium red staining reveals surface fibrils and a layer external to the cell wall in Streptococcus salivarius HB and adhesion deficient mutants. Journal of General Microbiology 134:3165–3172
    [Google Scholar]
  15. Harty D.W.S., Handley P.S. 1989; Expression of the surface properties of the fibrillar Streptococcus salivarius HB and its adhesion deficient mutants grown in continuous culture under glucose limitation. Journal of General Microbiology 135:2611–2621
    [Google Scholar]
  16. Haschemeyer R.H., Myers R.J. 1972; Negative staining. In Principles and Techniques of Electron Microscopy, Biological Applications, 2 pp 101–147 Hayat M. A. Edited by New York: Van Nostrand Reinhold;
    [Google Scholar]
  17. Honda T., Arita M., Miwatani T. 1984; Characterization of new hydrophobic pili of the enterotoxigenic Escherichia coli: a possible new colonization factor. Infection and Immunity 43:959–965
    [Google Scholar]
  18. Horne R.W. 1965a; The examination of small particles. In Techniques for Electron Microscopy,, 2nd edn.. pp 311–327 Kay D. H. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  19. Horne R.W. 1965b; Negative staining methods. In Techniques for Electron Microscopy,, 2nd edn.. pp 328–355 Kay D. H. Edited by Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  20. Jacobs A.A.C., de Graaf F.K. 1985; Production of K88, K99 and F41 fibrillae in relation to growth phase, and a rapid procedure for adhesin purification. FEMS Microbiology Letters 26:15–19
    [Google Scholar]
  21. Kasper D.L. 1976; The polysaccharide capsule of Bacteroides fragilissubspecies fragilis: immunochemical and morphologic detection. Journal of Infectious Diseases 133:79–87
    [Google Scholar]
  22. Kasper D.L., Onderdonk A.B., Polk B.F., Bartlett J.G. 1979; Surface factors as virulence factors in infection with Bacteroides fragilis. . Reviews of Infectious Diseases 1:278–288
    [Google Scholar]
  23. Kasper D.L., Onderdonk A.B., Reinap B.G., Lindberg A.A. 1980; Variations of Bacteroides fragilis with in vitro passage: presence of an outer membrane-associated glycan and loss of capsular antigen. Journal of Infectious Diseases 142:750–756
    [Google Scholar]
  24. Lindberg A.A., Weintraub A.B. 1985; Encapsulation and protection against phagocytosis by Bacteroides fragilis. . Scandinavian Journal of Infectious Diseases 46:27–32
    [Google Scholar]
  25. Lindberg A.A., Berthold P., Nord C., Weintraub A. 1979; Encapsulated strains of Bacteroides fragilis in clinical specimens. Medical Microbiology and Immunology 167:29–36
    [Google Scholar]
  26. Loesche W.J., Hockett R.N., Syed S.A. 1972; The predominant cultivable flora of tooth surface plaque removed from institutionalized subjects. Archives of Oral Biology 17:1311–1325
    [Google Scholar]
  27. Luft J.H. 1971; Ruthenium Red and Ruthenium Violet. I.Chemistry, purification, methods of use for electron microscopy and mechanisms of action. Anatomical Record 171:347–368
    [Google Scholar]
  28. Onderdonk A.B., Kasper R.L., Cisneros R.L., Bartlett J.G. 1977; The capsular polysaccharide of Bacteroides fragilis as a virulence factor: comparison of the pathogenic potential of encapsulated and unencapsulated strains. Journal of Infectious Diseases 136:82–89
    [Google Scholar]
  29. Onderdonk A.B., Moon N.E., Kasper D.L., Bartlett J.G. 1978; Adherence of Bacteroides fragilis in vivo. . Infection and Immunity 19:1083–1087
    [Google Scholar]
  30. Ottow J. 1975; Ecology, physiology and genetics of fimbriae and pili. Annual Review of Microbiology 29:79–108
    [Google Scholar]
  31. Paranchych W., Frost L.S. 1988; The physiology and biochemistry of pili. Advances in Microbial Physiology 29:53–114
    [Google Scholar]
  32. Patrick S., Reid J. H. 1983; Separation of capsulate and noncapsulate Bacteroides fragilis on a discontinuous density gradient. Journal of Medical Microbiology 16:239–241
    [Google Scholar]
  33. Patrick S., Reid J.H., Coffey A. 1986; Capsulation of in vitroand in vivo grown Bacteroides species. Journal of General Microbiology 132:1099–1109
    [Google Scholar]
  34. Patrick S., Coffey A., Emmerson A.M., Larkin M.J. 1988; The relationship between cell surface structure expression and haemagglutination in Bacteroides fragilis. . FEMS Microbiology Letters 50:67–71
    [Google Scholar]
  35. Pruzzo C., Dainelli B., Richetti M. 1984; Piliated Bacteroides fragilis strains adhere to epithelial cells and are more sensitive to phagocytosis by human neutrophils than non-piliated strains. Infection and Immunity 43:189–194
    [Google Scholar]
  36. Pruzzo C., Guzman C.A., Dainelli B. 1989; Incidence of haemagglutination activity among pathogenic and nonpathogenic Bacteroides fragilis strains and role of capsule and pili in HA and adherence. FEMS Microbiology Letters 59:113–118
    [Google Scholar]
  37. Riley T.V., Mee B.J. 1984; Haemagglutination of Bacteroides fragilis. . FEMS Microbiology Letters 25:229–232
    [Google Scholar]
  38. Rosenberg M., Gutnick D., Rosenberg E. 1980; Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiology Letters 9:29–33
    [Google Scholar]
  39. Sherman P.M., Houston W.L., Boedeker E.C. 1985; Functional heterogeneity of intestinal E. coli strains expressing type 1 somatic pili (fimbriae): assessment of bacterial adherence to intestinal membranes and surface hydrophobicity. Infection and Immunity 49:797–804
    [Google Scholar]
  40. Shinjo T., Kiyoyama H. 1984; Fimbriae and haemagglutination in the Bacteroides fragilis group. Japanese Journal of Veterinary Science 46:373–375
    [Google Scholar]
  41. Vel W.A.C., Namavar F., Marian A., Verweij-Van Vught J.J., Pubben A.B., MacLaren D.M. 1986; Haemagglutination by the Bacteroides fragilis group. Journal of Medical Microbiology 21:105–107
    [Google Scholar]
  42. Weerkamp A.H., Handley P.S., Baars A., Slot J.W. 1986; Negative-staining and immunoelectron microscopy of adhesion-deficient mutants of Streptococcus salivarius reveal that the adhesive protein antigens are separate classes of cell surface fibril. Journal of Bacteriology 165:746–755
    [Google Scholar]
  43. Weiss E., Rosenberg M., Judes H., Rosenberg E. 1982; Cell surface hydrophobicity of adherent oral bacteria. Current Microbiology 7:125–128
    [Google Scholar]
  44. Weiss E.I., London J., Kolenbrander P.E., Hand A.R., Siraganian R. 1988; Localization and enumeration of fimbria-associated adhesins of Bacteroides loeschii. . Journal of Bacteriology 170:1123–1128
    [Google Scholar]
  45. Yoshimura F., Takafiashi K., Nodasaka Y., Suzuki T. 1984; Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis. . Journal of Bacteriology 160:949–957
    [Google Scholar]
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