The enteric bacterium Salmonella typhimurium utilizes 1,2-propanediol as a sole carbon and energy source during aerobic growth, but only when the cells are also provided with cobalamin as a nutritional supplement. This metabolism is mediated by the cobalamin-dependent propanediol dehydratase enzyme pathway. Thirty-three insertion mutants were isolated that lacked the ability to utilize propanediol, but retained the ability to degrade propionate. This phenotype is consistent with specific blocks in one or more steps of the propanediol dehydratase pathway. Enzyme assays confirmed that propanediol dehydratase activity was absent in some of the mutants. Thus, the affected genes were designated pdu (for defects in propanediol utilization). Seventeen mutants carried pdu:: lac operon fusions, and these fusions were induced by propanediol in the culture medium. All of the pdu mutations were located in a single region (41 map units) on the S. typhimurium chromosome between the his (histidine biosynthesis) and branch I cob (cobalamin biosynthesis) operons. They were shown to be P22-cotransducible with a branch I cob marker at a mean frequency of 12%. Mutants that carried deletions of the genetic material between his and cob also failed to utilize propanediol as a sole carbon source. Based upon the formation of duplications and deletions between different pairs of his:: Mu dA insertions, the pdu genes were transcribed in a clockwise direction relative to the S. typhimurium genetic map.
AndersonR.P.,
RothJ.R.1978; Tandem chromosomal duplications in Salmonella typhimurium : fusion of histidine genes to novel promoters. Journal of Molecular Biology 119:147–166
BjörkG.R.1987; Modification of stable RNA. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 719–731NeidhardtF.C.,
IngrahamJ.L.,
LowK.B.,
MagasanikB.,
SchaechterM.,
UmbargerH.E.
Edited by Washington, DC:: American Society for Microbiology.;
CasadabanM.J.,
CohenS.N.1979; Lactose genes fused to exogenous promoters in one step using a Mu-lac bacteriophage: in vivo probe for transcriptional control sequences. Proceedings of the National Academy of Sciences of the United States of America 76:4530–4533
ForageR.G.,
FosterM.A.1982; Glycerol fermentation in Klebsiella pneumoniae: functions of the coenzyme B12-dependent glycerol and did dehydratases. Journal of Bacteriology 149:413–419
FreyB.,
McCloskeyJ.,
KerstenW.,
KerstenH.1988; New function of vitamin B12: cobamide-dependent reduction of epoxy-queuosine to queuosine in tRNAs of Escherichia coli and Salmonella typhimurium. Journal of Bacteriology 170:2078–2082
JeterR.M.,
OliveraB.M.,
RothJ.R.1984; Salmonella typhimurium synthesizes cobalamin (vitamin B12) de novo under anaerobic growth conditions. Journal of Bacteriology 159:206–213
JeterR.,
Escalante-SemerenaJ.C.,
RoofD.,
OliveraB.,
RothJ.1987; Synthesis and use of vitamin BI2. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 551–556NeidhardtF. C.,
IngrahamJ. L.,
LowK. B.,
MagasanikB.,
SchaechterM.,
UmbargerH. E.
Edited by Washington, DC: American Society for Microbiology;
LeeH.A.JrAbelesR.H.1963; Purification and properties of dioldehydrase, an enzyme requiring a cobamide coenzyme. Journal of Biological Chemistry 238:2367–2373
McgeeD.E.,
RichardsJ.H.1981; Purification and subunit characterization of propanediol dehydratase, a membrane-associated enzyme. Biochemistry 20:4293–4298
MaloyS.R.,
RothJ.R.1983; Regulation of proline utilization in Salmonella typhimurium: characterization of put :: :Mud(Ap, lac) operon fusions. Journal of Bacteriology 154:561–568
ObradorsN.,
BadÍaJ.,
BaldomÀL.,
AguilarJ.1988; Anaerobic metabolism of the l-rhamnose fermentation product 1,2-propanediol in Salmonella typhimurium. Journal of Bacteriology 170:2159–2162
PazM.A.,
BlumenfeldO.O.,
RojkindM.,
HensonE.,
FurfineC.,
GallopP.M.1965; Determination of carbonyl compounds with N-methylbenzothiazolone hydrazone. Archives of Biochemistry and Biophysics 109:548–559
SawickiE.,
HauserT.R.,
StanleyT.W.,
ElbertW.1961; The 3-methyl-2-benzothiazolone hydrazone test.Sensitive new methods for the detection, rapid estimation, and determination of aliphatic aldehydes. Analytical Chemistry 33:93–96
SodaK.1967; A spectrophotometric microdetermination of keto acids with 3-methyl-2-benzothiazolone hydrazone. Agricultural and Biological Chemistry 31:1054–1060
TorayaT.,
UshioK.,
FukuiS.,
HogenkampH.P.C.1977; Studies on the mechanism of the adenosylcobalamin-dependent diol dehydrase reaction by the use of analogs of the coenzyme. Journal of Biological Chemistry 252:963–970
TorayaT.,
HondaS.,
FukuiS.1979; Fermentation of 1,2-propanediol and 1,2-ethanediol by some genera of Enterobacteriaceae, involving coenzyme B12-dependent diol dehydratase. Journal of Bacteriology 139:39–47