SUMMARY: The conjugation mechanism of IncP plasmids may be employed to mobilize small non-conjugative plasmids from to a wide range of different organisms. This strategy has been adapted for use with the Grampositive anaerobe, NCIB 8052. Several shuttle vectors containing replicons from pAMß1 (), pCB101 () or pWV01 (), together with the -acting region of RK2, have been constructed, and transferred to and established in this organism. One of the vectors apparently contains a hot-spot for insertion of IS Conjugative mobilization of plasmids from will provide a useful alternative to electroporation for effecting gene transfer to this industrially important anaerobe.


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