SUMMARY: A gene coding for a thermostable extracellular α-amylase, carried by a 5·7 kb Hl chromosomal DNA fragment isolated from strain CUB74, was cloned into JM107 using, as a cloning vector, the high-copy-number plasmid pUC8. containing a recombinant plasmid pQR300 expressed the amylase gene and exported the enzyme into the periplasmic space and the culture medium. The amylase protein expressed by had the same molecular mass (50 kDa) as that expressed by the parent strain, which suggests that the enzyme is processed similarly by both strains. The amylase gene was also cloned into TK24 using pIJ702 as vector. The enzyme was stable at 70 °C when CaCl was present.


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