Nucleotide sequencing of DNA in the region of the Streptococcus mutans chromosome adjacent to the previously characterized gtfA gene revealed the presence of two long open reading frames. One of these corresponds to the dexB gene and has been shown to encode an intracellular exodextranase (dextran glucosidase) which has short isomaltosaccharides as preferred substrates. Comparison with other published sequences showed that the dexB gene product shares regions of similarity with enzymes, from a variety of sources, which attack other glucose polymers.
AmemuraA.,
ChakrabortyR.,
FujitaM.,
NoumiT.,
FutaiM.1988; Cloning and nucleotide sequence of the isoamylase gene from Pseudomonas amyloderamosa SB-15. Journal of Biological Chemistry 263:9271–9275
BarrettJ. F.,
CurtissR.1986; Renaturation of dextranase activity from culture supernatant fluids of Streptococcus sobrinus after sodium dodecylsulfate polyacrylamide gel electrophoresis. Analytical Biochemistry 158:365–370
BarrettJ. F.,
BarrettT. A.,
CurtissR.1987; Purification and partial characterization of the multicomponent dextranase complex of Streptococcus sobrinus and cloning of the dextranase gene. Infection and Immunity 55:792–802
BurneR. A.,
RubinfeldB.,
BowenW. H.,
YasbinR. E.1986; Tight genetic linkage of a glucosyltransferase and dextranase of Streptococcus mutans GS-5. Journal of Dental Research 65:1392–1401
FerrettiJ. J.,
GilmoreK. S.,
CourvalinP.1986; Nucleotide sequence analysis of the gene specifying the bifunctional 6′- aminoglycoside acetyltransferase 2”-aminoglycoside phosphotransferase enzyme in Streptococcus faecalis and identification and cloning of gene regions specifying the two activities. Journal of Bacteriology 167:631–638
FerrettiJ. J.,
HuangT. T.,
RussellR. R. B.1988; Sequence analysis of the glucosyltransferase A (gtfA) gene from Streptococcus mutans Ingbritt. Infection and Immunity 56:1585–1588
KatsuragiN.,
TakizawaN.,
MurookaY.1987; Entire nucleotide sequence of the pullulanase gene of Klebsiella aerogenes W70. Journal of Bacteriology 169:2301–2306
KurikiT.,
ImanakaT.1989; Nucleotide sequence of the neopullulanase gene from Bacillus stearothermophilus
. Journal of General Microbiology 135:1521–1528
LinderL.,
AnderssonC.,
SundM.,
ShockmanG. D.1983; Protoplast formation and localization of enzymes in Streptococcus mitis
. Infection and Immunity 40:1146–1154
MacKayR. M.,
BairdS.,
DoveM. J.,
ErrattJ. A.,
GinesM.,
MoranelliF.,
NasimA.,
WillickG. E.,
YaguchiM.,
SeligyV.1985; Glucanase gene diversity in prokaryotic and eukaryotic organisms. BioSystems 18:279–292
NakajimaR.,
ImanakaT.,
AibaS.1986; Comparison of amino acid sequences of eleven different α-amylases. Applied Microbiology and Biotechnology 23:355–360
RussellR. R. B.,
MorrisseyP.,
DouganG.1985; Cloning of sucrase genes from Streptococcus mutans in bacteriophage lambda. FEMS Microbiology Letters 30:37–41
SangerF.,
NicklenS.,
CoulsonA. R.1977; DNA sequencing with chain terminating inhibitors. Proceedings of the National Academy of Sciences of the United States of America 74:5463–5467
StaatR. H.,
SchachteleC. F.1974; Evaluation of dextranase production by the cariogenic bacterium Streptococcus mutans
. Infection and Immunity 9:467–469
SundM. L.,
BrantingC.,
LinderL. E.1989; Water-soluble glucans from Streptococcus mutans strain Ingbritt as an energy source for bacterial growth. Caries Research 23:256–260
TerleckyjB.,
WillettP. N.,
ShockmanG. D.1975; Growth of several cariogenic strains of oral streptococci in a chemically defined medium. Infection and Immunity 11:649–655
TognoniA.,
CarreraP.,
GalliG.,
LuccheseG.,
CameriniB.,
GrandiG.1989; Cloning and nucleotide sequence of the isoamylase gene from a strain of Pseudomonas sp. Journal of General Microbiology 135:37–45
WalkerG. J.,
PulknownikA.,
Morrey-JonesJ. G.1981; Metabolism of the polysaccharides of human dental plaque: release of dextranase in batch cultures of Streptococcus mutans
. Journal of General Microbiology 127:201–208
WilburW. J.,
LipmanD. J.1982; Rapid similarity searches of nucleic acid and protein data banks. Proceedings of the National Academy of Sciences of the United States of America 80:726–730