SUMMARY: The gene of is essential for bacteriophage λ DNA replication and is also necessary for host RNA and DNA synthesis at high temperature. A mutant of was found to be substantially more resistant to 50 °C heat treatment than the wild-type. Upon receiving a 42 °C heat shock for 15 min, both the wild-type and the mutant became more resistant to heat (i.e. they became thermotolerant). A revertant behaved similarly to the wild-type in that it was more sensitive to heat than cells. In addition, cells had the same HO and UV sensitivity as the wild-type. This implies that the conditions for which a mutation is beneficial are unique to heat exposure and are not caused by HO or UV exposure. Furthermore, synthesis of heat-shock proteins occurred sooner in the mutant than in the wild-type, indicating that the gene of may influence the regulation of the heat-shock response.


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