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Three species-specific, temperate actinophages of Streptomyces coelicolor Müller, ϕSC623, ϕSC347 and ϕSC681, were compared with respect to host range, virion structure, antiserum cross-inactivation, DNA-restriction pattern, DNA hybridization, and DNA base composition. The restriction map of ϕSC623 (57 kb) was established with eight restriction enzymes; the homologies of this phage with ϕSC347 and ϕSC681 suggested that it might be a hybrid phage composed of approximately equal parts homologous to one of the other two phages. No homology was detected between ϕSC623 and R4, a temperate, wide-host-range phage which can also lysogenize S. coelicolor Müller.
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