1887

Abstract

Monoclonal antibodies (mAbs) were raised against , a fungus commonly isolated from stored rice grains in South-East Asia. Mice were immunized by a direct, simple method; fresh cell-free surface washings from a solid agar slant culture were injected directly into the peritoneum without prior concentration. Hybridoma supernatants were screened by ELISA. Most of the mAbs raised cross-reacted with other storage fungi and/or uninfected rice grains but three were species-specific. One of these, PI01, was used to develop ELISA and DIP-STICK assays for the detection of in individual grains. All inoculated grains and approximately 90% of grains in natural infected samples from Indonesia tested positively, by ELISA, for . This result and those obtained for discoloured grains from both Indonesia and the Philippines, 32% and 14% respectively, are higher than those obtained by direct plating of surface-sterilized grains. Heat and periodate treatment of the PI01 antigen and binding on Western blots indicate that it is a glycoprotein of > 90000. Hyphae of all ages stained uniformly by immunofluorescence using the PI01 antibody but mature conidia stained only weakly.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-4-753
1990-04-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/4/mic-136-4-753.html?itemId=/content/journal/micro/10.1099/00221287-136-4-753&mimeType=html&fmt=ahah

References

  1. Cooney D.G., Emmerson R. 1964 Thermophilic Fungi. San Fransisco:: W. H.Freeman.;
    [Google Scholar]
  2. Dewey F.M., Barrett D.K., Vose I.R., Lamb C.J. 1984; Immunofluorescent microscopy for detection and identification of propagules of Phaeolus schweinitzii in infested soil. Phytopathology 74:291–296
    [Google Scholar]
  3. Dewey F.M., Brasier C.M. 1988; Development of ELISA for Ophiostoma ulmi using antigen coated wells. Plant Pathology 37:28–35
    [Google Scholar]
  4. Dewey F.M., Macdonald M.M., Phillips S.I. 1989a; Development of monoclonal-antibody-ELISA, -DOT-BLOT and -DIP-STICK immunoassays for Humicola lanuginosa in rice. Journal of General Microbiology 135:361–374
    [Google Scholar]
  5. Dewey F.M., Munday C.J., Brasier C.M. 1989b; Monoclonal antibodies to components of the Dutch Elm pathogen Ophiostoma ulmi. . Plant Pathology 38:9–20
    [Google Scholar]
  6. Foung S.K.H., Sasaki D.T., Engleman E.C. 1982; Production of functional human T-T hybridomas in selection medium lacking aminopterin and thymidine. Proceedings of the National Academy of Sciences of the United States of America 79:7484–7488
    [Google Scholar]
  7. Hardham A.R., Suzaki E., Perkin J.L. 1986; Monoclonal antibodies to isolate-, species-, and genus-specific components on the surface of zoospores and cysts of the fungus Phytophthora cinnamomi. . Canadian Journal of Botany 64:311–321
    [Google Scholar]
  8. Laemmli U.K. 1970; Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature; London: 227:680–685
    [Google Scholar]
  9. Lustgraaf B.V.D. 1978; Ecological relationships between xerophilic fungi and house-dust mites (Acarda pyroglyphidae). Oecologia 33:351–359
    [Google Scholar]
  10. MacDonald M.M., Dunstan R.H., Dewey F.M. 1989; Detection of low-Mr glycoproteins in surface washes of some fungal cultures by gel-filtration HPLC and by monoclonal antibodies. Journal of General Microbiology 135:375–383
    [Google Scholar]
  11. Moreau C. 1979; Islanditoxicosis. In Moulds, Toxins and Food, pp 190–196 Moss M. Edited by Chichester: John Wiley;
    [Google Scholar]
  12. Notermans S., Wieten G., Engel H.W.B., Rambouts R.M., Hoogerhout P., van Boom J.H. 1987; Purification and properties of extra-cellular polysaccharide (EPS) antigens produced by different mould species. Journal of Applied Bacteriology 62:157–166
    [Google Scholar]
  13. Phillips S.I., Mitfa R., Snitha S., Wallbridge A.J., Cooke R.D., Baker A.A. 1984; A study of the factors involved in the post-harvest yellowing of rice. In Health and Ecology in Grain Postharvest Technology, Proceedings of the 7 th ASEAN Technical Seminar, Kuala Lumpur: Malaysia;
    [Google Scholar]
  14. Phillips S.I., Widjaja S., Wallbridge A.J., Cooke R.D.C. 1988; Rice yellowing during post harvest drying by aeration and during storage. Journal of Stored Products Research 24:173–181
    [Google Scholar]
  15. Pitt J.I. 1979 The Genus Penicillium and its Teleomorphic States Eupenicillium and Telaromyces. London:: Academic Press.;
    [Google Scholar]
  16. Polonelli L., Castagnola M., Morace G. 1986; Identification and serotyping of Microsporum canis isolates by monoclonal antibodies. Journal of Clinical Microbiology 23:609–615
    [Google Scholar]
  17. Tsunoda H. 1953; Study of damage of stored rice, caused by microrganisms. II. On yellow rice from Thailand. Transactions of the Japanese Phytopathology Society 13:3
    [Google Scholar]
  18. Wycoff K.L., Jellison J., Ayers A.R. 1987; Monoclonal antibodies to glycoprotein antigens of a fungal plant pathogen, Phytophthora megasperma f. sp. glycinea. . Plant Physiology 85:508–515
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-4-753
Loading
/content/journal/micro/10.1099/00221287-136-4-753
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error