Summary: Non-motile mutants of were isolated after transposon insertion mutagenesis with either Tn5 on a plasmid or Tnp mini- in bacteriophage γ. The physical location and number of transposon insertions was determined. Eighteen Tn5 insertion mutants and 11 Tn10p mini- insertion mutants had single unique insertion sites. The 18 Tn5 insertions were contained within six different RI fragments and the 11 Tn10p mini- insertions were contained within eight different fragments of chromosomal DNA. These data suggest that multiple genes are involved in motility. Immunoblot analysis of non-motile mutants with antibody to wild-type flagellar core protein indicated that two of the non-motile mutants made flagellar core protein. Three additional mutants reacted weakly with the antibodies. However, these mutants with immunopositive reactions did not produce any structures which resembled flagella by transmission electron microscopy. In addition, none of the other non-motile mutants produced wild-type flagella. However, five mutants which did not react in the immunoblot produced a structure which resembled a flagellar sheath without the internal flagellar core. In addition to having no filamentous core, the sheaths often extended from the sides of the bacteria, rather than from the poles where the flagellum is normally located. The data suggest that sheath formation is independent of flagellar filament formation, but that proper positioning of the sheath may require the flagellar filament.


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