1887

Abstract

strain 206 degrades benzoate via the -cleavage pathway. In a genomic library derived from this organism a clone was obtained which carried and expressed the gene for the third enzyme in this pathway, catechol 2,3-dioxygenase (EC 1.13.11.2), on a 5·9 kb I restriction fragment. The structural gene was more precisely mapped on an internal 1·6 kb RI fragment which, after insertion into expression vectors, directed the synthesis of a 33 kDa polypeptide. The gene showed very little or no homology with isofunctional genes derived from Comprehensive substrate specificity analysis showed significant differences between the specific activities obtained from the cloned gene product and extracts derived from itself.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-4-607
1990-04-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/4/mic-136-4-607.html?itemId=/content/journal/micro/10.1099/00221287-136-4-607&mimeType=html&fmt=ahah

References

  1. Assinder S.J., Williams P.A. 1988; Comparisons of the metapathway operons on NAH plasmid pWW60-22 and TOL plasmid pWW53-4 and its evolutionary significance. Journal of General Microbiology 134:2769–2778
    [Google Scholar]
  2. Bagdasarian M., Franklin F. C. H., Lurz R., Rueckert B., Bagdasarian M. M., Timmis K. N. 1981); Specific purpose cloning vectors. II. Broad host range high copy numbers RSF1010- derived vectors and a host vector system for gene cloning. Gene 16:237–247
    [Google Scholar]
  3. Bagdasarian M.M., Amann E., Lurz R., Rueckert B., Bagdasarian M. 1983; Activity of the hybrid trp-lac (tac) promoter of Escherichia coli in Pseudomonas putida. Construction of broad-host-range, controlled expression vectors. Gene 26:273–282
    [Google Scholar]
  4. Benson S., Shapiro J. 1978; TOL is a broad-host-range plasmid. Journal of Bacteriology 135:278–280
    [Google Scholar]
  5. Bishop P.E., Jarlenski D.M.L., Hetherington D.R. 1980; Evidence for an alternative nitrogen fixation system in Azotobacter vinelandii. . Proceedings of the National Academy of Sciences of the United States of America 77:7342–7346
    [Google Scholar]
  6. Bishop P.E., Hawkins M.E., Eady R.R. 1986; Nitrogen fixation in molybdenum deficient continuous culture by a strain of Azotobacter vinelandii carrying a deletion of the structural genes for nitrogenase (nifHDK). . Biochemical Journal 238:437–442
    [Google Scholar]
  7. Bolivar F. 1978; Construction and characterization of new cloning vehicles. III. Derivatives of plasmid pBR322 carrying unique EcoRI sites for selection of EcoRI generated recombinant DNA molecules. Gene 4:121–136
    [Google Scholar]
  8. Bolivar F., Rodriguez R.L., Green P.J., Betlach H.C., Heynecker H.C., Boyer H.W., Crosa J.J., Falkow S. 1977; Construction and characterization of new cloning vehicles. II. A multi-purpose cloning system. Gene 2:95–113
    [Google Scholar]
  9. Borck J., Beggs J.D., Brammar W.J., Hopkins A.S., Murray N.E. 1976; The construction in vitro of transducing derivatives of phage lambda. Molecular and General Genetics 146:199–207
    [Google Scholar]
  10. Chatfield L.K., Williams P.A. 1986; Naturally occurring TOL plasmids in Pseudomonas strains carrying either two homologous or two non-homologous catechol 2,3-oxygenase genes. Journal of Bacteriology 168:878–885
    [Google Scholar]
  11. Chisnell J.R., Premakumar R., Bishop P.E. 1988; Purification of a second alternative nitrogenase from a nifHDK deletion strain of Azotobacter vinelandii. . Journal of Bacteriology 170:27–33
    [Google Scholar]
  12. Cohen S.N., Chang A.C.Y., Hsu C.L. 1972; Non chromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America 69:2110–2114
    [Google Scholar]
  13. Dagley S. 1986; Biochemistry of aromatic hydrocarbon degradation in Pseudomonas. . In The Biology of Pseudomonas: The Bacteria, 10 pp 527–555 Sokatch J. R. Edited by New York: Academic Press;
    [Google Scholar]
  14. Dunn N.W., Gunsalus I.C. 1973; Transmissible plasmid coding early enzymes of naphthalene oxidation in Pseudomonas putida. . Journal of Bacteriology 114:974–979
    [Google Scholar]
  15. Ghosal D., You I.S., Gunsalus I.C. 1987; Nucleotide sequence and expression of gene nahH of plasmid NAH7 and homology with gene xylE of TOL plasmid pWWO. Gene 55:19–26
    [Google Scholar]
  16. Harayama S., Don R.H. 1985; Catabolic plasmids: their analysis and utilization in the manipulation of bacterial metabolic activities. In Genetic Engineering, 7 pp 283–307 Setlow J. K., Hollaender A. Edited by New York: Plenum Press;
    [Google Scholar]
  17. Harayama S., Rekik M., Wasserfallen A., Bairoch A. 1987; Evolutionary relationships between catabolic pathways for aromatics : conservation of gene order and nucleotide sequences of catechol oxidation genes of pWWO and NAH7 plasmids. Molecular and General Genetics 210:241–247
    [Google Scholar]
  18. Hardisson C., Sala-Trepat J.M., Stanier R.Y. 1969; Pathways for the oxidation of aromatic compounds by Azotobacter. . Journal of General Microbiology 59:1–11
    [Google Scholar]
  19. Holmes D.S., Quigley M. 1981; A rapid boiling method for preparation of bacterial plasmids. Analytical Biochemistry 114:193–197
    [Google Scholar]
  20. Inouye S., Nakazawa A., Nakazawa T. 1981; Molecular cloning of TOL genes xylB and xylE in Escherichia coli. . Journal of Bacteriology 145:1137–1143
    [Google Scholar]
  21. Jacobsen M.R., Premakumar R., Bishop P.E. 1986; Transcriptional regulation of nitrogen fixation by molybdenum in Azotobacter vinelandii. . Journal of Bacteriology 167:480–486
    [Google Scholar]
  22. Keil H., Williams P.A. 1985; A new class of TOL plasmid deletion mutants in Pseudomonas putida MT15 and their reversion by tandem gene amplification,. Journal of General Microbiology 131:1023–1033
    [Google Scholar]
  23. Keil H., Lebens M.R., Williams P.A. 1985a; TOL plasmid pWW15 contains two nonhomologous, independently regulated catechol 2,3-oxygenase genes. Journal of Bacteriology 163:248–255
    [Google Scholar]
  24. Keil H., Keil S., Pickup R.W., Williams P.A. 1985b; Evolutionary conservation of genes coding for meta pathway enzymes within TOL plasmids pWWO and pWW53. Journal of Bacteriology 164:887–895
    [Google Scholar]
  25. Laemmli U.K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  26. Leeman R., Remaut E., Fiers W. 1987; A broad host range expression vector based on the pL promoter of coliphage: regulated synthesis of human interleukin 2 in Erwinia and Serratia species. Journal of Bacteriology 169:1899–1907
    [Google Scholar]
  27. Lehrbach P.R., McGregor I., Ward J.M., Broda P. 1983; Molecular relationships between Pseudomonas IncP-9 degradative plasmids TOL, NAH and SAL. Plasmid 10:164–174
    [Google Scholar]
  28. Maniatis T., Fritsch E.F., Sambrook J. 1982 Molecular Cloning: a Laboratory Manual, pp 98–148 Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory.;
    [Google Scholar]
  29. Messing J., Crea R., Seeburg P.H. 1981; A system for shotgun DNA sequencing. Nucleic Acids Research 9:309–321
    [Google Scholar]
  30. Nakai C., Kagamiyana H., Nozaki N., Nakazawa T., Inouye S., Ebina Y., Nakazawa A. 1983; Complete sequence of the meta- pyrocatechase gene on the TOL plasmid of Pseudomonas putida mt-2. Journal of Biological Chemistry 258:2923–2928
    [Google Scholar]
  31. Neidle E.L., Shapiro M.K., Ornston L.N. 1987; Cloning and expression in Escherichia coli of Acinetobacter calcoaceticusgenes for benzoate degradation. Journal of Bacteriology 169:5496–5503
    [Google Scholar]
  32. Reiner A.M. 1972; Metabolism of aromatic compounds in bacteria. Purification and properties of the catechol-forming enzyme, 3,5- cyclohexadiene-l,2-diol-l-carboxylic acid (NAD+) oxidoreductase (decarboxylating). Journal of Biological Chemistry 10:2530–2536
    [Google Scholar]
  33. Reiner A.M., Hegeman G.O. 1971; Metabolism of benzoic acid by bacteria. Accumulation of (-)-3,5-cyclohexadiene-l,2-diol-l- carboxylic acid by a mutant strain of Alcaligenes eutrophus. . Biochemistry 10:2530–2536
    [Google Scholar]
  34. Robson R.L. 1986; Nitrogen fixation in strains of Azotobacter chroococcum bearing deletions of a cluster of genes coding for nitrogenase. Archives of Microbiology 176:74–79
    [Google Scholar]
  35. Robson R.L., Chesshyre J.A., Wheeler C., Jones R., Woodley P.R., Postgate J.R. 1984; Genome size and complexity in Azotobacter chroococcum. . Journal of General Microbiology 130:1603–1612
    [Google Scholar]
  36. Sadoff H.L., Shimei B., Ellis S. 1979; Characterization of Azotobacter vinelandii deoxyribonucleic acid and folded chromosomes. Journal of Bacteriology 138:871–877
    [Google Scholar]
  37. Sala-Trepat J.M., Evans W.C. 1971; The meta cleavage of catechol by Azotobacter species. European Journal of Biochemistry 20:400–413
    [Google Scholar]
  38. Shanley M.S., Neidle E.L., Parales R.E., Ornston L.N. 1986; Cloning and expression of Acinetobacter calcoaceticus catBCDE genes in Pseudomonas putida and Escherichia coli. . Journal of Bacteriology 165:557–563
    [Google Scholar]
  39. Shaw L.E., Williams P.A. 1988; Physical and functional mapping of two cointegrate plasmids derived from RP4 and TOL plasmid pDKl. Journal of General Microbiology 134:2463–2474
    [Google Scholar]
  40. Tsuda M., Iino T. 1987; Genetic analysis of a transposon carrying toluene degrading genes on a TOL plasmid pWWO. Molecular and General Genetics 210:270–276
    [Google Scholar]
  41. Worsey M.J., Williams P.A. 1975; Metabolism of toluene and xylenes by Pseudomonas putida (arvilla) mt-2: evidence for a new function of the TOL plasmid. Journal of Bacteriology 124:7–13
    [Google Scholar]
  42. Worsey M.J., Franklin F.C.H., Williams P.A. 1978; Regulation of the degradative pathway enzymes coded for by the TOL plasmid (pWWO) from Pseudomonas putida mt-2. Journal of Bacteriology 134:757–764
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-4-607
Loading
/content/journal/micro/10.1099/00221287-136-4-607
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error