%0 Journal Article %A Hacket, Jacques %A Desmarais, Danielle %A Mehindate, Khalil %A Déry, Claude V. %T Saccharopolyspora Hirsuta Strain 367 Releases JHJ-1, a Bacteriophage Capable of Propagation on Old Mycelium %D 1990 %J Microbiology, %V 136 %N 3 %P 573-579 %@ 1465-2080 %R https://doi.org/10.1099/00221287-136-3-573 %I Microbiology Society, %X Bacteriophage JHJ-1 was isolated from Saccharopolyspora hirsuta strain 367 NRRL 12045 as an endogenous but virulent phage. The plaque size was not self-limiting, since a few p.f.u. could completely lyse a lawn. Electron microscopy showed that this phage belonged to group B of Bradley’s morphological classification. The JHJ-1 genome is a linear DNA molecule of 41·1 kbp with cohesive ends and a G + C content of 68·8–70·0 mol%. The DNA cleavage was established for 12 restriction endonucleases. The host range is apparently very narrow, being limited to two strains of S. hirsuta (NRRL 12045 and NRRL B-5792). However, JHJ-1 did not lytically infect S. hirsuta 367 UC 8106. Phage JHJ-1 was shown, by Southern blot analysis, to lysogenize both S. hirsuta strain 367 UC 8106. It thus appears to behave as a virulent mutant of a temperate phage on one, but not on the other, JHJ-1 lysogen. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-3-573