1887

Abstract

produces an inducible -lactamase and induction with 100 μg ampicillin ml resulted in an increase of more than 100-fold in enzyme activity. Various other -lactam antibiotics also served as effective inducers. The enzyme was obtained from cells by osmotic shocking to release periplasmic components and it was purified primarily by ion-exchange chromatography and PAGE. The -lactamase consists of one polypeptide with a molecular mass of about 28 kDa and an isoelectric point greater than 9·6. It is strongly inhibited by -chloromercuribenzoate and clavulanic acid but not by EDTA. The enzyme readily hydrolyses several penicillins and cephalosporins, but not oxacillin or cloxacillin. The enzyme therefore belongs to group 2b of the bacterial -lactamases.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-3-521
1990-03-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/3/mic-136-3-521.html?itemId=/content/journal/micro/10.1099/00221287-136-3-521&mimeType=html&fmt=ahah

References

  1. Barlow G. H., Margoliash E. 1966; Electrophoretic behavior of mammalian-type cytochrome c. Journal of Biological Chemistry 241:1473–1477
    [Google Scholar]
  2. Bio-Rad. 1988 Chromatography, Electrophoresis, Immunochemistry, Molecular Biology, HPLC, Liquid handling Catalogue N p. 102
    [Google Scholar]
  3. Bush K. 1989a; Characterization of /5-lactamases. Antimicrobial Agents and Chemotherapy 33:259–263
    [Google Scholar]
  4. Bush K. 1989b; Classification of β-lactamases: groups 1,2a, 2b, and 2b´. Antimicrobial Agents and Chemotherapy 33:264–270
    [Google Scholar]
  5. Bush K. 1989c; Classification of β-lactamases: groups 2c, 2d, 2e, 3, and 4. Antimicrobial Agents and Chemotherapy 33:271–276
    [Google Scholar]
  6. Cheng K.-J., Ingram J. M., Costertqn J. W. 1970; Release of alkaline phosphatase from cells of Pseudomonas aeruginosa by manipulation of cation concentration and of pH. Journal of Bacteriology 104:748–753
    [Google Scholar]
  7. Christensen P., Cook F. D. 1978; Lysobacter, a new genus of nonfruiting gliding bacteria with a high base ratio. International Journal of Systematic Bacteriology 28:367–393
    [Google Scholar]
  8. Demoss R. D. 1955; Glucose-6-phosphate and 6-phosphogluconic dehydrogenase from Leuconostoc mesenteroides . Methods in Enzymology 1:328–334
    [Google Scholar]
  9. Fairbanks G., Steck T. L., Wallach D.F.H. 1971; Electrophoretic analysis of major polypeptides of the human erythrocytic membrane. Biochemistry 10:2606–2616
    [Google Scholar]
  10. Hedges A., Wolfe R. S. 1974; Extracellular enzyme from Myxobacter AL-1 that exhibits both β-1,4-glucanase and chitosanase activities. Journal of Bacteriology 120:844–853
    [Google Scholar]
  11. Hirai K., Iyobe S., Inoue M., Mitsuhashi S. 1980; Purification and properties of a new /1-lactamase from Pseudomonas cepacia . Antimicrobial Agents and Chemotherapy 17:355–358
    [Google Scholar]
  12. Jackson R. L., Matsueda G. R. 1970; Myxobacter AL-1 protease. Methods in Enzymology 19:591–599
    [Google Scholar]
  13. Maizel J. V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:179–246
    [Google Scholar]
  14. Neu H. L., Heppel L. A. 1965; The release of enzymes from Escherichia coli by osmotic shock and during formation of sphero-plasts. Journal of Biological Chemistry 246:3685–3692
    [Google Scholar]
  15. O’Callaghan C. H., Muggleton P. W., Ross G. W. 1968; Effects of β-lactamase from Gram-negative organisms on cephalosporins and penicillins. In Antimicrobial Agents and Chemotherapy pp 57–63 Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Reichenbach H., Dworkin M. 1981; The order Cytophagales (with addenda on the genera Herpetosiphon, Saprospira, and Flexithrix) . In The Prokaryotes pp 356–379 Starr M. P., Stolp H., Truper H. G., Balows A., Schlegel H. G. Edited by New York: Springer-Verlag;
    [Google Scholar]
  17. Richards F. M., Wyckoff H. W. 1971; Bovine pancreatic ribonuclease. In The Enzymes IV pp 647–806 Boyer P. Edited by New York: Academic Press;
    [Google Scholar]
  18. Richmond M. H., Sykes E. B. 1973; The β-lactamases of Gramnegative bacteria and their possible physiological role. Advances in Microbial Physiology 9:31–88
    [Google Scholar]
  19. Rosenberg E., Varon M. 1984; Antibiotics and lytic enzymes. In Myxobacteria, Development and Cell Interactions pp 109–125 Rosenberg E. Edited by New York: Springer-Verlag;
    [Google Scholar]
  20. Ross G. W., O’Callaghan C. H. 1975; β-Lactamase assays. Methods in Enzymology 43:69–85
    [Google Scholar]
  21. Samuni A. 1975; A direct spectrophotometric assay and determination of Michaelis constants for the β-lactamase reaction. Analytical Biochemistry 63:17–26
    [Google Scholar]
  22. Sanders C. C. 1987; Chromosomal cephalosporinases responsible for multiple resistance to newer β-lactam antibiotics. Annual Review of Microbiology 41:573–593
    [Google Scholar]
  23. Von Tigerstrom R. G. 1980; Extracellular nucleases of Lysobacter enzymogenes: production of the enzymes and purification and characterization of an endonuclease. Canadian Journal of Microbiology 26:1029–1037
    [Google Scholar]
  24. Von Tigerstrom R. G. 1981; Extracellular nucleases of Lysobacter enzymogenes: purification and characterization of a ribonuclease. Canadian Journal of Microbiology 27:1080–1086
    [Google Scholar]
  25. Von Tigerstrom R. G. 1984; Production of two phosphatases by Lysobacter enzymogenes and purification and characterization of the extracellular enzyme. Applied and Environmental Microbiology 47:693–698
    [Google Scholar]
  26. Von Tigerstrom R. G., Stelmaschuk S. 1985; Localization of the cell-associated phosphatase in Lysobacter enzymogenes . Journal of General Microbiology 131:1611–1618
    [Google Scholar]
  27. Von Tigerstrom R. G., Stelmaschuk S. 1986; Purification and characterization of the outer membrane associated alkaline phosphatase of Lysobacter enzymogenes . Journal of General Microbiology 132:1379–1387
    [Google Scholar]
  28. Von Tigerstrom R. G., Stelmaschuk S. 1987; Purification and partial characterization of an amylase from Lysobacter brunescens . Journal of General Microbiology 133:3437–3443
    [Google Scholar]
  29. Von Tigerstrom R. G., Stelmaschuk S. 1989; Localization and characterization of lipolytic enzymes produced by Lysobacter enzymogenes . Journal of General Microbiology 135:1027–1035
    [Google Scholar]
  30. Waley S. G. 1974; A spectrophotometric assay of β-lactamase action on penicillins. Biochemical Journal 139:789–790
    [Google Scholar]
  31. Warburg O., Christian W. 1941; Isolierung and Kristallisation des Garungsferments Enolase. Biochemische Zeitschrift 310:384–421
    [Google Scholar]
  32. Whitaker D. R. 1970; The α-lytic protease of a myxobacterium. Methods in Enzymology 19:599–613
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-3-521
Loading
/content/journal/micro/10.1099/00221287-136-3-521
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error