Biochemical and Immunological Comparison of Lipopolysaccharides from Species Free

Abstract

Lipopolysaccharides (LPS) isolated from and were analysed for their chemical composition, molecular heterogeneity and immunological properties. All the LPS preparations contained heptose, 3-deoxy---2-octulosonic acid, glucosamine, uronic acid, phosphate and fatty acids. The fatty acids C14:0, C16:0 and OHC14:0 were common to all the LPS preparations. LPS from strains additionally contained isoC16:0, those from contained isoC14:0 and isoC16:0, and those from contained C16:1. By SDS-PAGE, LPS from had two bands of low molecular mass, and the LPS from and showed low molecular mass bands together with a ladder arrangement of high molecular mass bands. Immunodiffusion, quantitative agglutination and ELISA demonstrated that the LPS from strains reacted with antisera prepared against whole cells of and ; LPS from reacted with antisera to and , and LPS from reacted with anti-whole cell serum raised against any of the three species. From these results, it is concluded that LPS from has structures in common with LPS from and , while the LPS from and are serologically entirely different from each other.

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1990-03-01
2024-03-28
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