1887

Abstract

The energetics of were studied in anaerobic glucose-limited chemostat cultures via an analysis of biomass and metabolite production. The observed was dependent on the composition of the biomass, the production of acetate, the extracellular pH, and the provision of an adequate amount of fatty acid in the medium. Under optimal growth conditions, the was approximately 16 g biomass (mol ATP formed). This is much higher than previously reported for batch cultures. Addition of acetic acid or propionic acid lowered the . A linear correlation was found between the energy required to compensate for import of protons and the amount of acid added. This energy requirement may be regarded as a maintenance energy, since it was independent of the dilution rate at a given acid concentration.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-3-405
1990-03-01
2021-08-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/3/mic-136-3-405.html?itemId=/content/journal/micro/10.1099/00221287-136-3-405&mimeType=html&fmt=ahah

References

  1. Aiking H., Tempest D. W. 1976; Growth and physiology of Candida utilis NCYC 321 in potassium-limited chemostat culture. Archives of Microbiology 108:117–124
    [Google Scholar]
  2. Alexander B., Leach S., Ingledew W. J. 1987; The relationship between chemiosmotic parameters and sensitivity to anions and organic acids in the acidophile Thiobacillus ferrooxidans. Journal of General Microbiology 133:1171–1179
    [Google Scholar]
  3. Alroy Y., Tannenbaum S. R. 1973; The influence of environmental conditions on the macromolecular composition of Candida utilis. Biotechnology and Bioengineering 15:239–256
    [Google Scholar]
  4. Andreasen A. A., Stier T.J.B. 1953; Anaerobic nutrition of Saccharomyces cerevisiae. I. Ergosterol requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 41:23–36
    [Google Scholar]
  5. Andreasen A. A., Stier T.J.B. 1954; Anaerobic nutrition of Saccharomyces cerevisiae. II. Unsaturated fatty acid requirement for growth in a defined medium. Journal of Cellular and Comparative Physiology 43:271–281
    [Google Scholar]
  6. Andrews G. 1989; Estimating cell and product yields. Biotechnology and Bioengineering 33:256–265
    [Google Scholar]
  7. Atkinson B., Mavituna F. 1983 In Biochemical Engineering and Biotechnology Handbook pp. 143–147181–182 Byfleet, Surrey:: Macmillan/Globe Book Services.;
    [Google Scholar]
  8. Baronofsky J. J., Schreurs W.J.A., Kashket E. R. 1984; Uncoupling by acetic acid limits growth of and acetogenesis by Clostridium thermoaceticum. Applied and Environmental Microbiology 48:1134–1139
    [Google Scholar]
  9. Barford J. P., Hall R. J. 1981; A mathematical model for the aerobic growth of Saccharomyces cerevisiae with a saturated respiratory capacity. Biotechnology and Bioengineering 23:1735–1762
    [Google Scholar]
  10. Bauchop T., Elsden S. R. 1960; The growth of micro-organisms in relation to their energy supply. Journal of General Microbiology 23:457–469
    [Google Scholar]
  11. Bruinenberg P. M., Van Dijken J. P., Scheffers W. A. 1983; A theoretical analysis of NADPH production and consumption in yeasts. Journal of General Microbiology 129:953–964
    [Google Scholar]
  12. Cassio F., LeÃo C., Van Uden N. 1987; Transport of lactate and other short-chain monocarboxylates in the yeast Saccharomyces cerevisiae. Applied and Environmental Microbiology 53:509–513
    [Google Scholar]
  13. Chistyakova T. I., Dedyukhina É.G., Il’Chenko V. Y., Eroshin V. K. 1983; Effects of the rate of growth on the composition of biomass in Candida valida. Microbiology 51:620–624
    [Google Scholar]
  14. Conway E. J., Armstrong W. McD. 1961; The total intracellular concentration of solutes in yeast and other plant cells and the distensibility of the plant-cell wall. Biochemical Journal 81:631–639
    [Google Scholar]
  15. Dekkers J.G.J., De Kok H. E., Roels J. A. 1981; Energetics of Saccharomyces cerevisiae CBS 426: comparison of anaerobic and aerobic glucose limitation. Biotechnology and Bioengineering 23:1023–1035
    [Google Scholar]
  16. Dufour J.-P., Goffeau A., Tsong T. Y. 1982; Active proton uptake in lipid vesicles reconstituted with the purified yeast plasma membrane ATPase. Journal of Biological Chemistry 257:9365–9371
    [Google Scholar]
  17. Eddy A. A. 1982; Mechanisms of solute transport in selected eukaryotic micro-organisms. Advances in Microbial Physiology 23:178
    [Google Scholar]
  18. Eraso P., Gancedo C. 1987; Activation of yeast plasma membrane ATPase by acid pH during growth. FEBS tetters 224:187–192
    [Google Scholar]
  19. Eraso P., Cid A., Serrano R. 1987; Tight control of the amount of yeast plasma membrane ATPase during changes in growth conditions and gene dosage. FEBS tetters 224:193–197
    [Google Scholar]
  20. Furukawa K., Heinzle E., Dunn I. J. 1983; Influence of oxygen on the growth of Saccharomyces cerevisiae in continuous culture. Biotechnology and Bioengineering 25:2293–2317
    [Google Scholar]
  21. Goffeau A., Slayman C. W. 1981; The proton-translocating ATPase of the fungal plasma membrane. Biochimica et Biophysica Acta 639:197–223
    [Google Scholar]
  22. Haukeli A. D., Lie S. 1971; Molar growth yields of yeasts in anaerobic batch cultures. Journal of General Microbiology 69:135–141
    [Google Scholar]
  23. KormanČÍkovÁ V., KovÁČ L., VidovÁ M. 1969; Oxidative phosphorylation in yeast. V. Phosphorylation efficiencies in growing cells determined from molar growth yields. Biochimica et BiophysicaActa 180:9–17
    [Google Scholar]
  24. Krebs H. A., Wiggins D., Stubbs M. 1983; Studies on the mechanism of the antifungal action of benzoate. Biochemical Journal 214:657–663
    [Google Scholar]
  25. Lagunas R. 1976; Energy metabolism of Saccharomyces cerevisiae: discrepancy between ATP balance and known metabolic functions. Biochimica et BiophysicaActa 440:661–674
    [Google Scholar]
  26. Lang J. M., Cirillo V. P. 1987; Glucose transport in a kinaseless Saccharomyces cerevisiae mutant. Journal of Bacteriology 169:2932–2937
    [Google Scholar]
  27. Maiorella B. L., Blanch H. W., Wilke C. R. 1984; Feed component inhibition in ethanolic fermentation by Saccharomyces cerevisiae. Biotechnology and Bioengineering 26:1155–1166
    [Google Scholar]
  28. Malpartida F., Serrano R. 1981; Proton translocation catalyzed by the purified yeast plasma membrane ATPase reconstituted in liposomes. FEBS Letters 131:351–354
    [Google Scholar]
  29. Nelson N., Taiz L. 1989; The evolution of H+-ATPases. Trends in Biochemical Sciences 14:113–116
    [Google Scholar]
  30. Oura E. 1972 The effect of aeration on the growth energetics and biochemical composition of baker’s yeast. PhD thesis University of Helsinki, Finland.:
    [Google Scholar]
  31. Oura E. 1977; Reaction products of yeast fermentations. Process Biochemistry 12(3):19–2135
    [Google Scholar]
  32. Parada G., Acevedo F. 1983; On the relation of temperature and RNA content to the specific growth rate in Saccharomyces cerevisiae. Biotechnology and Bioengineering 25:2785–2788
    [Google Scholar]
  33. Perlin D. S., San Francisco M.J.D., Slayman C. W., Rosen B. P. 1986; H+/ATP stoichiometry of proton pumps from Neurospora crassa and Escherichia coli. Archives of Biochemistry and Biophysics 248:53–61
    [Google Scholar]
  34. Pirt S. J. 1965; The maintenance energy of bacteria in growing cultures. Proceedings of the Royal Society of London 163B:224–231
    [Google Scholar]
  35. Postma E., Verduyn C., Scheffers W. A., Van Dijken J. P. 1989; Enzymic analysis of the Crabtree effect in glucose-limited chemostat cultures of Saccharomyces cerevisiae. Applied and Environmental Microbiology 55:468–477
    [Google Scholar]
  36. Rogers P. J., Stewart P. R. 1974; Energetic efficiency and maintenance energy characteristics of Saccharomyces cerevisiae (wild type and petite) and Candida parapsilosis grown aerobically and micro-aerobically in continuous culture. Archives of Microbiology 99:25–46
    [Google Scholar]
  37. Romano A. H. 1982; Facilitated diffusion of 6-deoxy-d-glucose in bakers’ yeast: evidence against phosphorylation-associated transport of glucose. Journal of Bacteriology 152:1295–1297
    [Google Scholar]
  38. Schatzmann H. 1975 Anaerobes Wachstum von Saccharomyces cerevisiae. PhD thesis Eidgenussische Technische Hochschule Zurich, Switzerland.:
    [Google Scholar]
  39. Serrano R., Kielland-Brandt M. C., Fink G. R. 1986; Yeast plasma membrane ATPase is essential for growth and has homology with (Na+ + K+), K+-and Ca2+-ATPases. Nature; London: 319689–693
    [Google Scholar]
  40. Stanier R. Y., Ingraham J. L., Wheelis M. L., Painter P. R. 1987 In General Microbiology, 5th edn. pp. 108–112134–142 Basingstoke:: Macmillan;
    [Google Scholar]
  41. Stouthamer A. H. 1973; A theoretical study on the amount of ATP required for synthesis of microbial cell material. Antonie van Leeuwenhoek 39:545–565
    [Google Scholar]
  42. Stouthamer A. H. 1979; The search for correlation between theoretical and experimental growth yields. Microbial Biochemistry 21:1–47
    [Google Scholar]
  43. Stouthamer A., Bettenhaussen C. 1973; Utilization of energy for growth and maintenance in continuous and batch cultures of microorganisms. Biochimica et Biophysica Acta 301:53–70
    [Google Scholar]
  44. Tempest D. W., Neijssel O. 1984; The status of YATP and maintenance energy as biologically interpretable phenomena. Annual Review of Microbiology 38:459–486
    [Google Scholar]
  45. Umbarger H. E. 1978; Amino acid biosynthesis and its regulation. Annual Review of Biochemistry 47:533–606
    [Google Scholar]
  46. Vallejo C. G., Serrano R. 1989; Physiology of mutants with reduced expression of plasma membrane H+-ATPase. Yeast 5:307–319
    [Google Scholar]
  47. Verduyn C., Postma E., Scheffers W. A., Van Dijken J. P. 1990; Physiology of Saccharomyces cerevisiae in anaerobic glucose-limited chemostat cultures. Journal of General Microbiology 136:395–403
    [Google Scholar]
  48. Viegas C. A., Rosa F. M., SÁ-Correia I., Novais J. M. 1989; Inhibition of yeast growth by octanoic and decanoic acids produced during ethanolic fermentation. Applied and Environmental Microbiology 55:21–28
    [Google Scholar]
  49. Warth A. D. 1977; Mechanism of resistance of Saccharomyces bailii to benzoic, sorbic and other weak acids used as food preservatives. Journal of Applied Bacteriology 43:215–230
    [Google Scholar]
  50. Warth A. D. 1988; Effect of benzoic acid on growth yield of yeasts differing in their resistance to preservatives. Applied and Environmental Microbiology 54:2091–2095
    [Google Scholar]
  51. Warth A. D. 1989; Transport of benzoic and propanoic acids by Zygosaccharomyces bailii. Journal of General Microbiology 135:1383–1390
    [Google Scholar]
  52. Watson T. G. 1970; Effects of sodium chloride on steady-state growth and metabolism of Saccharomyces cerevisiae. Journal of General Microbiology 64:91–99
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-3-405
Loading
/content/journal/micro/10.1099/00221287-136-3-405
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error