@article{mbs:/content/journal/micro/10.1099/00221287-136-2-319, author = "Fujiwara, Taku and Ogawa, Tomohiko and Sobue, Shizuo and Hamada, Shigeyuki", title = "Chemical, immunobiological and antigenic characterizations of lipopolysaccharides from Bacteroides gingivalis strains", journal= "Microbiology", year = "1990", volume = "136", number = "2", pages = "319-326", doi = "https://doi.org/10.1099/00221287-136-2-319", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-2-319", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Lipopolysaccharides (LPS) were extracted from whole cells of seven strains of Bacteroides gingivalis - 381, ATCC 33277, BH18/10, OMZ314, OMZ409, 6/26 and HW24D-1 - by the phenol/water procedure, and purified by treatment with nuclease and by repeated ultracentrifugation. These LPS were composed of hexoses, hexosamines, fatty acids, phosphorus and phosphorylated 2-keto-3-deoxyoctonate (KDO). The major components of the lipid portion of these LPS were hexadecanoic, 3-hydroxyhexadecanoic, branched 3-hydroxypentadecanoic and branched 3-hydroxyheptadecanoic acids. All the LPS preparations induced marked mitogenic and in vitro polyclonal B cell activation responses in spleen cells from both C3H/HeN and C3H/HeJ mice, exhibited no definitive preparatory activity in the local Shwartzman reaction in rabbits, but were active in the chromogenic Limulus amoebocyte lysate test. A monoclonal antibody (mAb) raised against the LPS from B. gingivalis strain 6/26 reacted with LPS from all other B. gingivalis strains tested. Other mAbs raised against LPS from B. gingivalis strains 381 and 6/26 reacted with the LPS from strains 381, ATCC 33277, BH18/10 and 6/26 (these strains were termed LPS serogroup I), as revealed by ELISA and immunodiffusion. The LPS from these strains except for 6/26 showed almost indentical patterns in SDS-PAGE stained with ammoniacal silver. A mAb raised against the LPS from B. gingivalis HW24D-1 reacted with the LPS from strains OMZ314, HW24D-1 and OMZ409 (LPS serogroup II). These LPS, except OMZ409, exhibited very similar profiles in SDS-PAGE. These results indicate that there are at least two different antigenic groups present among LPS from B. gingivalis strains, as well as a common, species-specific antigen.", }