1887

Abstract

SUMMARY: The gene encoding the major secreted protein MPB70 was cloned and isolated from a DNA library in λ EMBL 3, and the restriction map of the area of chromosome containing the gene ascertained. After sub-cloning, the complete DNA sequence and predicted amino acid sequence were determined, and from this information a series of overlapping octapeptides encoding all possible linear epitopes of eight or less amino acids were synthesized. These peptides were probed with monoclonal antibodies specific for and with sera from -infected cattle. Epitopes defined by this technique were then examined using a substitution analysis that allowed the significance of each amino acid in the putative epitope to be quantified, and the exact specificity of the antibody response for the epitope determined.

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/content/journal/micro/10.1099/00221287-136-2-265
1990-02-01
2019-11-22
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http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-2-265
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