Summary: Two DNA probes for the detection of insertion sequence IS by either Southern blotting or colony hybridization were constructed. One of the probes is a 300 bp RI-III fragment of IS cloned onto pBluescript KS(+); the other is a tail-to-tail dimer of the same fragment cloned onto pUC19. A survey of the presence of IS among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of spp. examined contained one or more copies of insertion sequence IS, with the exception of the subgenus I serovar in which IS is not found. Although insertion sequence IS was first considered a Salmonella-specific element, it also exists in many isolates of and , but not in .


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