1887

Abstract

Two DNA probes for the detection of insertion sequence IS by either Southern blotting or colony hybridization were constructed. One of the probes is a 300 bp RI-dIII fragment of IS cloned onto pBluescript KS(+); the other is a tail-to-tail dimer of the same fragment cloned onto pUC19. A survey of the presence of IS among enteric bacteria revealed that more than 90% of the pathogenic or food-poisoning isolates of spp. examined contained one or more copies of insertion sequence IS, with the exception of the subgenus I serovar in which IS is not found. Although insertion sequence IS was first considered a Salmonella-specific element, it also exists in many isolates of and , but not in .

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-12-2555
1990-12-01
2021-05-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/12/mic-136-12-2555.html?itemId=/content/journal/micro/10.1099/00221287-136-12-2555&mimeType=html&fmt=ahah

References

  1. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  2. Brenner D. J. 1984; Enterobacteriaceae. In Bergey’s Manual of Systematic Bacteriology pp. 408–420 Krieg N. R., Holt J. G. Edited by Baltimore & London: Williams & Wilkins;
    [Google Scholar]
  3. Brenner D. J., Fanning G. R., Steigerwalt A. G., Sodd M. A., Doctor B. P. 1977; Conservation of transfer ribonucleic acid and 5S ribonucleic acid cistrons in Enterobacteriaceae . Journal of Bacteriology 129:1435–1439
    [Google Scholar]
  4. Casadesús J., Roth J. R. 1989; Absence of insertions among spontaneous mutants of Salmonella typhimurium . Molecular and General Genetics 216:210–216
    [Google Scholar]
  5. Chaconas G., Van De Sande J. H. 1980; 5′ 32P-labeling of RNA and DNA restriction fragments. Methods in Enzymology 65:75–88
    [Google Scholar]
  6. Chan R. K., Botstein D., Watanabe T., Ogata Y. 1972; Specialized transduction of tetracycline by phage P22 in Salmonella typhimurium. II. Properties of a high frequency transducing lysate. Virology 50:883–898
    [Google Scholar]
  7. Crawford I. P., Nichols B. P., Yanofsky C. 1980; Nucleotide sequence of the trpB gene in Escherichia coli and Salmonella typhimurium . Journal of Molecular Biology 142:489–502
    [Google Scholar]
  8. Davis R. W., Botstein D., Roth J. R. 1980 Advanced Bacterial Genetics Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Foster T., Davis M. A., Takeshita K., Roberts D. E., Kleckner N. 1981; Genetic organization of transposon Tn10 . Cell 23:201–213
    [Google Scholar]
  10. Galas D. J., Chandler M. 1989; Bacterial insertion sequences. In Mobile DNA pp. 109–162 Berg D. E., Howe M. M. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  11. Hanahan D., Meselson M. 1980; Plasmid screening at high colony density. Gene 19:147–151
    [Google Scholar]
  12. Johnston M. H., Roth J. R. 1979; Histidine mutants requiring adenine: selection of mutants with reduced hisG expression in Salmonella typhimurium . Genetics 92:1–15
    [Google Scholar]
  13. Jones D., Sneath P. A. H. 1970; Genetic transfer and bacterial taxonomy. Bacteriological Reviews 34:40–81
    [Google Scholar]
  14. Lam S., Roth J. R. 1983a; IS200: a Salmonella-specific insertion sequence. Cell 34:951–960
    [Google Scholar]
  15. Lam S., Roth J. R. 1983b; Genetic mapping of IS200 copies in Salmonella typhimurium strain LT2. Genetics 105:801–811
    [Google Scholar]
  16. Lam S., Roth J. R. 1986; Structural and functional studies on insertion element IS200 . Journal of Molecular Biology 187:157–167
    [Google Scholar]
  17. Maniatis T., Fritsch E. F., Sambrook J. 1982 Molecular Cloning: A laboratory Manual Cold Spring Harbor, New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  18. Matsutani S., Othsubo H., Maeda Y., Othsubo E. 1987; Isolation and characterization of IS elements repeated in the bacterial chromosome. Journal of Molecular Biology 196:445–455
    [Google Scholar]
  19. Ochman H., Wilson A. C. 1987; Evolutionary history of enteric bacteria. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp. 1649–1654 Neidhardt F. C. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  20. Roth J. R., Schmid M. B. 1981; Arrangement and rearrangement of the bacterial chromosome. Stadler Symposia 13:53–69
    [Google Scholar]
  21. Schmieger H. 1971; A method for the detection of phage mutants with altered transducing ability. Molecular and General Genetics 110:378–381
    [Google Scholar]
  22. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  23. Vogel H. G., Bonner D. M. 1956; Acetylomithase of E. coli: partial purification and some properties. Journal of Biological Chemistry 218:97–106
    [Google Scholar]
  24. Wilson K. 1987; Preparation of genomic DNA from bacteria. In Current Protocols in Molecular Biology pp. 2·4·1–2·4·2 Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. D., Smith J. A., Struhl K. Edited by New York: Wiley;
    [Google Scholar]
  25. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-12-2555
Loading
/content/journal/micro/10.1099/00221287-136-12-2555
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error