1887

Abstract

In medium of low or high osmolality, bacteroids isolated from nodules induced by 102F34 rapidly catabolized [C]choline. Trimethylamine was never detected and glycine betaine was the predominant product within the ethanol-soluble fraction of bacteroids subjected to salt stress (0·4 -NaCl). Both choline oxidase and glycine betaine aldehyde dehydrogenase activities were characterized; the apparent values for choline and glycine betaine aldehyde were 2·0 and 0·4 m, respectively. A 3 h incubation of the bacteroids in medium of high osmolarity, supplemented or not with choline, did not significantly modify the specific activity of the two enzymes. Similarly, salinization of the host plants for 2 weeks with 0·15 -NaCl had only a slight effect on both enzymic activities. Thus, the choline-glycine betaine biosynthetic pathway was not modulated by the external osmotic pressure. Glycine betaine itself was actively degraded by bacteroids suspended in low-osmolarity medium, but the demethylation process producing sarcosine and glycine was extremely slow in bacteroids subjected to salt stress. Thus, high concentration of glycine betaine can be maintained in salt-stressed bacteroids.

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1990-12-01
2022-01-26
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References

  1. Ahmad N., Wyn Jones R. G. 1979; Comparative turnover of glycine betaine and proline in barley plants released from salt and osmotic stress. Plant Science Letters 15:231–237
    [Google Scholar]
  2. Bernard T., Pocard J. A., Perroud B., Le Rudulier D. 1986; Variations in the response of salt-stressed Rhizobium strains to betaines. Archives of Microbiology 143:359–364
    [Google Scholar]
  3. Bowman M. S., Rohringer R. 1970; Formate metabolism in healthy and rust-infected wheat. Canadian Journal of Botany 48:803–811
    [Google Scholar]
  4. Coughlan S. J., Wyn Jones R. G. 1982; Glycine betaine biosynthesis and its control in detached secondary leaves of spinach. Planta 154:6–17
    [Google Scholar]
  5. Fougère F., Le Rudulier D. 1990; Uptake of glycine betaine and its analogues by bacteroids of Rhizobium meliloti . Journal of General Microbiology 136:157–163
    [Google Scholar]
  6. Grumet R., Hanson A. D. 1986; Genetic evidence for an osmoregulatory role of glycine betaine accumulation in barley. Australian Journal of Plant Physiology 13:353–364
    [Google Scholar]
  7. Guerin V., Trinchant J. C., Rigaud J. 1990; Nitrogen fixation (C2H2 reduction) by broad bean (Viciafaba L.) nodules and bacteroids under water restricted conditions. Plant Physiology 92:595–601
    [Google Scholar]
  8. Hanson A. D., Hitz W. D. 1982; Metabolic responses of mesophytes to plant water deficits. Annual Review of Plant Physiology 33:163–203
    [Google Scholar]
  9. Hanson A. D., Nelsen C. E. 1978; Betaine accumulation and [14C]formate metabolism in water-stressed barley leaves. Plant Physiology 62:305–312
    [Google Scholar]
  10. Hanson A. D., Rhodes D. 1983; 14C tracer evidence for synthesis of choline and betaine via phosphoryl base intermediates in salinized sugarbeet leaves. Plant Physiology 71:692–700
    [Google Scholar]
  11. Hanson A. D., Scott N. A. 1980; Betaine synthesis from radioactive precursors in attached, water-stressed barley leaves. Plant Physiology 66:342–348
    [Google Scholar]
  12. Hanson A. D., Wyse R. 1982; Biosynthesis, translocation, and accumulation of betaine in sugar beet and its progenitors in relation to salinity. Plant Physiology 70:1191–1198
    [Google Scholar]
  13. Hanson A. D., May A. M., Grumet R., Bode J., Jamieson G. C., Rhodes D. 1985; Betaine synthesis in chenopods: localization in chloroplasts. Proceedings of the National Academy of Sciences of the United States of America 82:3678–3682
    [Google Scholar]
  14. Hitz W. D., Rhodes D., Hanson A. D. 1981; Radiotracer evidence implicating phosphoryl and phosphatidyl bases as intermediate in betaine synthesis by water-stressed barley leaves. Plant Physiology 68:814–822
    [Google Scholar]
  15. Kates M., Marshall M. O. 1975; Biosynthesis of phosphoglycerides in plants. In Recent Advances in the Chemistry and Biochemistry of Lipids pp. 115–159 Galliard T., Mercer E. I. Edited by London: Academic Press;
    [Google Scholar]
  16. Ladyman J. A. R., Hitz W. D., Hanson A. D. 1980; Translocation and metabolism of glycine betaine by barley plants in relation to water stress. Planta 150:191–196
    [Google Scholar]
  17. Landfald B., Strøm A. R. 1986; Choline-glycine betaine pathway confers a high level of osmotic tolerance in Escherichia coli . Journal of Bacteriology 165:849–855
    [Google Scholar]
  18. Le Rudulier D., Bouillard L. 1983; Glycine betaine, an osmotic effector in Klebsiella pneumoniae and other members of the Enterobacteriaceae . Applied and Environmental Microbiology 46:152–159
    [Google Scholar]
  19. Le Rudulier D., Yang S. S., Csonka L. N. 1982; Nitrogen fixation in Klebsiella pneumoniae during osmotic stress. Effect of exogenous proline or a proline overproducing plasmid. Biochimica et Biophysica Acta 719:273–283
    [Google Scholar]
  20. Le Rudulier D., Strøm A. R., Dandekar A. M., Smith L. T., Valentine R. C. 1984; Molecular biology of osmoregulation. Science 224:1064–1068
    [Google Scholar]
  21. Macdonnell E., Wyn Jones R. G. 1988; Glycine betaine biosynthesis and accumulation in unstressed and salt-stressed wheat. Journal of Experimental Botany 39:421–430
    [Google Scholar]
  22. Maizel J. V., Benson A. A., Tolbert N. E. 1956; Identification of phosphorylcholine as an important constituent of plant saps. Plant Physiology 31:407–408
    [Google Scholar]
  23. Perroud B., Le Rudulier D. 1985; Glycine betaine transport in Escherichia coli: osmotic modulation. Journal of Bacteriology 161:393–401
    [Google Scholar]
  24. Pocard J. A., Bernard T., Goas G., Le Rudulier D. 1984; Restauration partielle, par la glycine bétaineet la proline bétaine, de l’activité fixatrice d’azote de jeunesplantes de Medicago sativa L. soumises a un stress hydrique. Comptes-Rendus de l’Académie des Sciences 298:477–480
    [Google Scholar]
  25. Rigaud J. 1976; Effet des nitrates sur la fixation d’azote par les nodules de Haricot (Phaseolus vulgaris L.). Physiologie Végétale 14:297–308
    [Google Scholar]
  26. Sethi J. K., Carew D. P. 1974; Growth and betaine formation in Medicago sativa tissue cultures. Phytochemistry 13:321–324
    [Google Scholar]
  27. Smith L. T., Pocard J. A., Bernard T., Le Rudulier D. 1988; Osmotic control of glycine betaine biosynthesis and degradation in Rhizobium meliloti . Journal of Bacteriology 170:3142–3149
    [Google Scholar]
  28. Weigel P., Weretilnyk E. A., Hanson A. D. 1986; Betaine aldehyde oxidation by spinach chloroplasts. Plant Physiology 82:753–759
    [Google Scholar]
  29. Weigel P., Lerma C., Hanson A. D. 1988; Choline oxidation by intact spinach chloroplasts. Plant Physiology 86:54–60
    [Google Scholar]
  30. Weretilnyk E. A., Hanson A. D. 1989; Betaine aldehyde dehydrogenase from spinach leaves: purification, in vitro translation of the mRNA, and regulation by salinity. Archives of Biochemistry and Biophysics 271:56–63
    [Google Scholar]
  31. Willemot C., Verret G. 1973; Incorporation of choline-1,2-14C into molecular species of phosphatidylcholine by alfalfa leaflet tissue. Lipids 8:588–591
    [Google Scholar]
  32. Wyn Jones R. G., Storey R. 1981; Betaines. In Physiology and Biochemistry of Drought Resistance in Plants pp. 171–204 Paleg L. G., Aspinall D. Edited by Sydney: Academic Press;
    [Google Scholar]
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