1887

Abstract

The lectin in is a major protein, although its function was previously unknown. An enzyme-linked immunosorbent assay was developed to quantify the lectin content in mycelium, sclerotia and culture filtrate during the development of grown in a synthetic medium. Lectin content was low in young mycelium but increased dramatically at the onset of sclerotium formation, reaching a maximum in adult sclerotia. Upon myceliogenic germination, the lectin content of the sclerotia rapidly decreased. It thus appears that the lectin is a developmental regulated sclerotium-specific protein accumulating during sclerotium formation and disappearing during germination. Its relative abundance and the pattern of developmental control suggests that the lectin is probably a storage protein. Lectin accumulation was also influenced by the composition of the medium. Addition of supplementary carbohydrate (-glucose) caused a marked reduction in lectin content whereas increased nitrogen in the form of -asparagine led to a higher lectin content. However, when the -asparagine concentration was too high, autolysis occurred and part of the lectin was released into the medium.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-12-2489
1990-12-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/12/mic-136-12-2489.html?itemId=/content/journal/micro/10.1099/00221287-136-12-2489&mimeType=html&fmt=ahah

References

  1. Barak R., Elad Y., Mirelman D., Chet I. 1985; Lectins: a possible basis for specific recognition in the interaction of Trichoderma and Sclerotium rolfsii . Phytopathology 75:458–462
    [Google Scholar]
  2. Barak R., Elad Y., Chet I. 1986; The proterties of l-fucose binding agglutinin associated with the cell wall of Rhizoctonia solani . Archives of Microbiology 144:346–349
    [Google Scholar]
  3. Bullock S., Ashford A. E., Willets H. J. 1980; The structure and histochemistry of sclerotia of Sclerotinia minor Jagger. II. Histochemistry of extracellular substances and cytoplasmic reserves. Protoplasma 104:333–351
    [Google Scholar]
  4. Chet I., Henis Y., Kislev N. 1969; Ultrastructure of sclerotia and hyphae of Sclerotium rolfsii Sacc. Journal of General Microbiology 57:143–147
    [Google Scholar]
  5. Christias C., Lockwood J. L. 1973; Conservation of mycelial constituents in four sclerotium-forming fungi in nutrient-deprived conditions. Phytopathology 63:602–605
    [Google Scholar]
  6. Insell J. P., Huner N. P. A., Newsted W. J., Huystee R. B. 1985; Light microscopic and polypeptide analysis of sclerotia from mesophilic and psychrophilic fungi. Canadian Journal of Botany 63:2305–2310
    [Google Scholar]
  7. Ishikawa F., Oishi K. 1989; Production, purification and characterization of Neurospora sitophila lectin. Agricultural and Biological Chemistry 53:1769–1776
    [Google Scholar]
  8. Kyhse-Anderson J. 1988; Semi-dry electroblotting transfer using equipment without buffer vessels. In CRC Handbook of Immunoblotting 1 pp. 79–85 Heegard N., Bjerrum O. J. Edited by Boca Raton, Florida: CRC Press;
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature; London: 227680–685
    [Google Scholar]
  10. Lahoz R., Miralles M. 1970; Influence of the level of the carbon source on the autolysis of Aspergillus niger . Journal of General Microbiology 62:271–276
    [Google Scholar]
  11. Latgé J. -P., Monsigny M., Prevost M. -C. 1988; Visualization of exocellular lectins in the entomopathogenic fungus Conidiobolus obscurus . Journal of Histochemistry and Cytochemistry 36:1419–1424
    [Google Scholar]
  12. Lockhart C. M., Rowell P., Stewart W. D. P. 1978; Phytohaemagglutinin from TM nitrogen-fixing lichens Peltigera canina and Peltigera polydactyla . FEMS Microbiology Letters 3:127–130
    [Google Scholar]
  13. Nordbring-Hertz B., Mattiasson B. 1979; Action of a nematode-trapping fungus shows lectin-mediated host-microorganism interaction. Nature; London: 281477–419
    [Google Scholar]
  14. Petersen G. R., Russo G. M., Van Etten J. 1982; Identification of major proteins in sclerotia of Sclerotinia minor and Sclerotinia trifoliorum . Experimental Mycology 6:268–273
    [Google Scholar]
  15. Peumans W. J., Nsimba-Lubaki M., Broekaert W. F., Van Damme E. J. M. 1986; Are bark lectins of elderberry (Sambucus nigra) and black locust (Robinia pseudoacacia) storage proteins?. In Molecular Biology of Seed Storage Proteins and lectins pp. 53–63 Shannon L. M., Chrispeels M. J. Edited by Baltimore, MD: Waverly Press;
    [Google Scholar]
  16. Rosenzweig W. D., Premachandran D., Pramer D. 1985; Role of trap lectins in the specificity of nematode capture by fungi. Canadian Journal of Microbiology 31:693–695
    [Google Scholar]
  17. Russo G. M., Dahlberg K. R., Van Etten J. 1982; Identification of a development-specific protein in sclerotia of Sclerotinia sclerotiorum . Experimental Mycology 6:259–267
    [Google Scholar]
  18. Terrance K., Heller P., Wu Y. -S., Lipke P. N. 1987; Identification of glycoprotein components of α-agglutinin, a cell adhesion protein from Saccharomyces cerevisiae . Journal of Bacteriology 169:475–482
    [Google Scholar]
  19. Townsend B. B. 1957; Nutritional factors influencing the production of sclerotia by certain fungi. Annals of Botany 21:153–166
    [Google Scholar]
  20. Van Damme E. J. M., Peumans W. J. 1989; Developmental changes and tissue distribution of lectin in Tulipa . Planta 178:10–18
    [Google Scholar]
  21. Voller A., Bidwell D., Bartlett A. 1980; Enzyme-linked immunosorbent assay. In Manual of Clinical Immunology pp. 359–371 Rose N. R., Friedman H. Edited by Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Vranken A. M., Van Damme E. J. M., Allen A. K., Peumans W. J. 1987; Purification and properties of an N-acetylgalactosamine specific lectin from the plant pathogenic fungus Rhizoctonia solani . FEBS Letters 216:67–72
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-12-2489
Loading
/content/journal/micro/10.1099/00221287-136-12-2489
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error