A combined physical and genetic map of Pseudomonas aeruginosa PAO was constructed by pulsed-field gel electrophoresis and Southern hybridization using cosmid clones from a genomic library carrying known genes. A total of 37 SpeI restriction fragments have been mapped on the 5862 kb genome, and fragment contiguity demonstrated by hybridization with clones from a SpeI junction fragment library and fragments obtained by partial SpeI digestion, both derived from the P. aeruginosa PAO chromosome.
AllenL.,
HansonR. S.1985; Construction of broad-host-range cosmid cloning vectors: identification of genes necessary for growth of Methylobacterium organophilum on methanol. Journal of Bacteriology 161:955–962
BancroftI.,
WolkP.,
OrenE. V.1989; Physical and genetic maps of the genome of the heterocyst-forming cyanobacterium Anabaena sp. strain PCC7120. Journal of Bacteriology 171:5940–5948
BrayR.,
StromD.,
BartonJ.,
DeanH. F.,
MorganA. F.1987; Isolation and characterization of Pseudomonas putida R-prime plasmids. Journal of General Microbiology 133:683–690
ChandlerR. M.,
KrishnapillaiV.1974; Phenotypic properties of R factors readily transferable between Pseudomonas and the Enterobacteriaceae
. Genetical Research 23:239–250
ElyB.,
ElyT. W.,
GerardotC. J.,
DingwallA.1990; Circularity of the Caulobacter cresceritus chromosome determined by pulsed-field gel electrophoresis. Journal of Bacteriology 172:1262–1266
FrühR.,
HaasD.,
LeisingerT.1985; Altered control of glutamate dehydrogenases in ornithine utilisation mutants of Pseudomonas aeruginosa
. Archives of Microbiology 141:170–176
FrutosR.,
PapesM.,
BellisM.,
RoizesG.,
BergoinM.1989; Pulsed-field gel electrophoresis determination of the genome size of obligate intracellular bacteria belonging to the genera Chlamydia, Rickettsiella and Porochlamydia
. Journal of Bacteriology 171:4511–4513
HollowayB. W.1965; Variations in restriction and modification of bacteriophage following increase of growth temperature of Pseudomonas aeruginosa
. Virology 25:634–642
HollowayB. W.,
ZhangC.1990; Pseudomonas aeruginosa PAO. In Genetic Maps Locus Maps of Complex Genomes, 5th edn. pp. 2·71–2·78O’BrienS. J.
Edited by Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
HollowayB. W.,
DharmsthitiS.,
JohnsonC.,
KearneyA.,
KrishnapillaiV.,
MorganA. F.,
RatnaningsihE.,
SafferyR.,
SinclairM.,
StromD.,
ZhangC.1990; Chromosome organization in Pseudomonas aeruginosa and Pseudomonas putida
. In Pseudomonas: Biotransformations, Pathogenesis and Evolving Biotechnology pp. 269–278SilverS.,
ChakrabartyA.,
IglewskiB.,
KaplanS.
Edited by Washington, DC: American Society for Microbiology;
LeeJ. L.,
SmithH. O.1988; Sizing of the Haemophilus influenzae Rd genome by pulsed-field agarose gel electrophoresis. Journal of Bacteriology 170:4402–4405
LeisingerT.,
HaasD.,
HegartyM. P.1972; Indospicine as an arginine antagonist in Escherichia coli and Pseudomonas aeruginosa
. Biochimica et Biophysica Acta 262:214–219
LyonB. R.,
KearneyP. P.,
SinclairM. I.,
HollowayB. W.1988; Comparative complementation mapping of Methylophilus spp. using cosmid clone libraries and prime plasmids. Journal of General Microbiology 134:123–132
McclellandM.,
JonesR.,
PatelY.,
NelsonM.1987; Restriction endonucleases for pulsed field mapping of bacterial genomes. Nucleic Acids Research 15:5985–6005
MeileL.,
LeisingerT.1982; Purification and properties of the bifunctional proline dehydrogenase/1-pyrroline-5-carboxylate dehydrogenase from Pseudomonas aeruginosa
. European Journal of Biochemistry 129:67–75
MorganA. F.1982; Isolation and characterisation of Pseudomonas aeruginosa R′ plasmids constructed by interspecific mating. Journal of Bacteriology 149:654–661
MorganA. F.,
DeanH. F.1985; Chromosome map of Pseudomonas putida PPN, and a comparison of gene order with the Pseudomonas aeruginosa PAO chromosomal map. Journal of General Microbiology 131:885–896
NayuduM.,
HollowayB. W.1981; Isolation and characterization of R-plasmid variants with enhanced chromosomal mobilization ability in Escherichia coli K12. Plasmid 6:53–66
O’HoyK.,
KrishnapillaiV.1985; Transposon mutagenesis of the Pseudomonas aeruginosa PAO chromosome and the isolation of high frequency of recombination donors. FEMS Microbiology tetters 29:299–303
O’HoyK.,
KrishnapillaiV.1987; Recalibration of the Pseudomonas aeruginosa strain PAO chromosome map in time units using high-frequency-of-recombination donors. Genetics 115:611–618
RoehlR. A.,
PhibbsP. V.1982; Characterization and genetic mapping of fructose phosphotransferase mutations in Pseudomonas aeruginosa
. Journal of Bacteriology 149:897–905
ScottK. F.,
RolfeB. G.,
ShineJ.1981; Biological nitrogen fixation: primary structure of the Klebsiella pneumoniae nifH and nifD genes. Journal of Molecular and Applied Genetics 1:71–81
SimonR.,
PrieferV.,
PuhlerA.1983; A broad host range mobilisation system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology 1:784–791
SmithC. L.,
WarburtonP. E.,
GallA.,
CantorC. R.1986; Analysis of genome organization and rearrangements by pulsed field gradient gel electrophoresis. Genetic Engineering 8:45–70
SuwantoA.,
KaplanS.1989; Physical and genetic mapping of the Rhodobacter sphaeroides 2·4·1 genome: the presence of two unique circular chromosomes. Journal of Bacteriology 171:5850–5859
WaterburyP. G.,
LaneM. J.1987; Generation of lambda phage concatemer for use as pulsed field electrophoresis size markers. Nucleic Acid Research 15:3930