A yeast with unusual sulphur amino acid metabolism Free

Abstract

A yeast strain highly resistant to propargylglycine (an inhibitor of cystathionine -lyase) was isolated from air. It was partially characterized, but it has not been identified with any known yeast species. Its sulphur amino acid metabolism differed from that of other fungi by the lack of the reverse transsulphuration pathway from methionine to cysteine, as no activity of cystathionine -synthase or cystathionine -lyase was found. The functional lack of this pathway was confirmed by growth tests and by experiments with [S]methionine. In contrast to neither homocysteine synthase nor the sulphate assimilation pathway were repressible by methionine in the new strain; on the contrary, a regulatory effect of cysteine was observed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-11-2283
1990-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/11/mic-136-11-2283.html?itemId=/content/journal/micro/10.1099/00221287-136-11-2283&mimeType=html&fmt=ahah

References

  1. Barnett J. A., Payne R. W., Yarrow D. 1983 Yeasts, Characteristics and Identification Cambridge: Cambridge University Press;
    [Google Scholar]
  2. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 18:248–254
    [Google Scholar]
  3. Breton A., Surdin-Kerjan Y. 1977; Sulphate uptake in Saccharomyces cerevisiae: biochemical and genetic study. Journal of Bacteriology 132:428–435
    [Google Scholar]
  4. Cherest H., Surdin-Kerjan Y., De Robichon-Szulmajster H. 1975; Methionine- and S-adenosylmethionine-mediated repression in methionyl-transfer ribonucleic acid synthetase mutant of Saccharomyces cerevisiae . Journal of Bacteriology 123:428–435
    [Google Scholar]
  5. Morzycka E., Sawnor-Korszynska D., Paszewski A., Grabski J., Raczynska Bojanowska J. 1976; Methionine overproduction by Saccharomycopsis lipolytica . Journal of Applied and Environmental Microbiology 32:125–130
    [Google Scholar]
  6. Ono B., Suruga T., Yamamoto M., Mutata K., Kimura A., Shinoda S., Ohmori S. 1984; Cystathionine accumulation in Saccharomyces cerevisiae . Journal of Bacteriology 158:860–865
    [Google Scholar]
  7. Paszewski A., Grabski J. 1973; Studies on γ-cystathionase and O-acetylhomoserine sulfhydrylase as the enzymes of alternative biosynthetic pathway in Aspergillus nidulans . Acta Biochimica Polonica 20:159–168
    [Google Scholar]
  8. Paszewski A., Grabski J. 1974; Regulation of S-amino acids biosynthesis in Aspergillus nidulans . Molecular and General Genetics 132:307–320
    [Google Scholar]
  9. Paszewski A., Prazmo W., Nadolska J., Regulski M. 1984; Mutations affecting the sulphur assimilation pathway in Aspergillus nidulans: their effect on sulphur amino acid metabolism. Journal of General Microbiology 130:1113–1121
    [Google Scholar]
  10. Pieniazek N. J., Paszewski A. 1970; The use of media containing sodium selenate for selecting mutants of Aspergillus nidulans . Aspergillus News Letter 11:12
    [Google Scholar]
  11. Pieniazek N. J., Stepien P. P., Paszewski A. 1973; Aspergillus nidulans mutant lacking cystathionine synthase. Biochimica et Biophysica Acta 297:34–47
    [Google Scholar]
  12. Piotrowska M., Paszewski A. 1986; Propargylglycine as a fungal inhibitor: effect on sulphur amino acid metabolism. Journal of General Microbiology 132:2753–2760
    [Google Scholar]
  13. Swietlinska Z., Zaborowska D., Haladus E., Zuk J. 1978; Study on liquid holding recovery in DEB-inactivated rad-3 mutant of Saccharomyces cerevisiae . Molecular and General Genetics 166:97–107
    [Google Scholar]
  14. Wiebers J. L., Garner Y. 1967; Acyl derivatives of homoserine as substrate for homocysteine synthesis in Neurospora crassa, yeast, and Escherichia coli . Journal of Biological Chemistry 242:5644–5649
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-11-2283
Loading
/content/journal/micro/10.1099/00221287-136-11-2283
Loading

Data & Media loading...

Most cited Most Cited RSS feed