1887

Abstract

Activities of the polyol dehydrogenases of f. sp. were surveyed by measuring polyol-dependent rates of reduction of NAD and NADP in cell-free extracts of axenically-grown mycelia. Seven of the eight polyols tested caused NADP reduction, with highest activity for -glucitol, followed by -arabitol, xylitol, erythritol, galactitol and ribitol, and low activity with -arabitol; only -mannitol failed to support activity. Inactivation rates were consistent with at least three separate enzymes, specific for -glucitol, xylitol and -arabitol respectively, with apparent values of 170–198 m for xylitol and -glucitol ( for NADP 36–55 µM), and 34 m for -arabitol ( for NADP 1·2 µM). The NADP-dependent activities were almost completely inhibited by 2 m-dithiothreitol, in contrast to the NAD-dependent activities, which were stimulated. NAD-dependent activity was highest with -mannitol, followed by successively lower activities with -arabitol, xylitol and -glucitol, with no activity with any of the other polyols; each of the four active polyols appeared to be oxidized by a different enzyme. All four NAD-dependent activities were rapidly lost after Sephadex treatment of crude extracts.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-136-11-2275
1990-11-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/136/11/mic-136-11-2275.html?itemId=/content/journal/micro/10.1099/00221287-136-11-2275&mimeType=html&fmt=ahah

References

  1. Adomako D., Kaye M. A. G., Lewis D. H. 1972; Carbohydrate metabolism in Chaetomium globosum . New Phytologist 71:467–476
    [Google Scholar]
  2. Arsenis C., Maniatis T., Touster O. 1968; Intramitochondrial location of the nicotinamide adenine dinucleotide- and nicotinamide adenine dinucleotide phosphate-dependent xylitol dehydrogenases. Journal of Biological Chemistry 244:4396–4399
    [Google Scholar]
  3. Barnett J. A. 1968; The catabolism of acyclic polyols by yeast. Journal of General Microbiology 52:131–159
    [Google Scholar]
  4. Chiang C., Knight S. G. 1961; l-Arabinose metabolism by cell- free extracts of Penicillium chrysogenum . Biochimica et Biophysica Acta 46:271–278
    [Google Scholar]
  5. Clancy F. G., Coffey M. D. 1980; Polyol dehydrogenases in the rust fungus, Melampsora lini (Ehrenb.) Lév. Journal of General Microbiology 120:85–88
    [Google Scholar]
  6. Duggleby R. G. 1984; Regression analysis of nonlinear Arrhenius plots: an empirical model and a computer program. Computers in Biology and Medicine 14:447–455
    [Google Scholar]
  7. Hollmann S., Latterman D. 1969; Oxydation von l-Arabit durch eine Polyalkohol-Dehydrogenase aus Hammelsamenblasen. Hoppe-Seyler’s Zeitschrift für Physiologische Chemie 350:51–56
    [Google Scholar]
  8. Isenberg P., Niederpruem D. J. 1967; Control of erythritol dehydrogenase in Schizophyllum commune . Archiv für Mikrobiologie 56:22–30
    [Google Scholar]
  9. Lewis D. H., Smith D. C. 1967; Sugar alcohols (polyols) in fungi and green plants. I. Distribution, physiology and metabolism. New Phytologist 66:143–184
    [Google Scholar]
  10. Maclean D. J. 1971 Studies on axenic cultures of the wheat stem rust fungus PhD thesis University of Queensland:
    [Google Scholar]
  11. Maclean D. J. 1974; Cultural and nutritional studies on variant strains of the wheat stem rust fungus. Transactions of the British Mycological Society 62:333–349
    [Google Scholar]
  12. Maclean D. J. 1982; Axenic culture and metabolism of rust fungi. In The Rust Fungi pp. 37–120 Scott K. J., Chakravorty A. K. Edited by London: Academic Press;
    [Google Scholar]
  13. Maclean D. J., Scott K. J. 1970; Variant forms of saprophytic mycelium grown from uredospores of Puccinia graminis f. sp. tritici . Journal of General Microbiology 64:19–27
    [Google Scholar]
  14. Maclean D. J., Scott K. J. 1976; Identification of glucitol (sorbitol) and ribitol in a rust fungus, Puccinia graminis f. sp. tritici . Journal of General Microbiology 97:83–89
    [Google Scholar]
  15. Manners J. M., Maclean D. J., Scott K. J. 1982; Pathways of glucose assimilation in Puccinia graminis . Journal of General Microbiology 128:2621–2630
    [Google Scholar]
  16. Manners J. M., Maclean D. J., Scott K. J. 1984; Hexitols as major intermediates of glucose assimilation by mycelium of Puccinia graminis . Archives of Microbiology 139:158–162
    [Google Scholar]
  17. Manners J. M., Maclean D. J., Scott K. J. 1988; Metabolism of 2-deoxy-d-glucose by axenically grown mycelia of Puccinia graminis . Experimental Mycology 12:350–356
    [Google Scholar]
  18. Mckay D. B. 1990 Characterization of pathways of carbohydrate assimilation by the wheat stem rust fungus MSc thesis University of Queensland:
    [Google Scholar]
  19. Morton N., Dickerson A. G., Hammond J. B. W. 1985; Mannitol metabolism in Agaricus bisporus: purification and properties of mannitol dehydrogenase. Journal of General Microbiology 131:2885–2890
    [Google Scholar]
  20. Pfyffer G. E., Pfyffer B. U., Rast D. M. 1986; The polyol pattern, chemotaxonomy, and phylogeny of the fungi. Sydowia 39:160–201
    [Google Scholar]
  21. Singh M., Scrutton N. S., Scrutton M. C. 1988; NADPH generation in Aspergillus nidulans: is the mannitol cycle involved?. Journal of General Microbiology 134:643–654
    [Google Scholar]
  22. Touster O., Shaw D. R. D. 1962; Biochemistry of the acyclic polyols. Physiological Reviews 42:181–225
    [Google Scholar]
  23. Wynn W. K. 1966; NAD- and NADP-linked dehydrogenases from bean rust. Plant Physiology 41:xxvi abstract
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-136-11-2275
Loading
/content/journal/micro/10.1099/00221287-136-11-2275
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error