A bacteriolytic muramidase from the basidiomycete Free

Abstract

The basidiomycete produces an extracellular bacteriolytic enzyme when grown on heat-killed cells of as sole C, N and P source. The enzyme catalyses the dissolution of isolated cell walls at an optimum pH of 3·2–3·4, releasing muramyl reducing groups, which indicates that it is a muramidase. Although low levels of enzyme activity are present when the fungus is grown in the absence of bacteria, full enzyme production appears to be induced by bacterial cells and repressed by glucose. Whole bacteria are not lysed by the enzyme at pH 3·3, but are rendered osmotically fragile, and lyse when the pH is raised to 7 or higher. The muramidase is effective against several Gram-positive bacteria but did not lyse any of the Gramnegative species tested.

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1990-11-01
2024-03-29
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References

  1. Anderson A. J., Green R. S., Sturman A. J., Archibald A. R. 1978; Cell wall assembly in Bacillus subtilis: location of wall material incorporated during pulsed release of phosphate limitation, its accessibility to bacteriophages and concanavalin A, and its susceptibility to turnover. Journal of Bacteriology 136:886–899
    [Google Scholar]
  2. Blake C. C. F., Johnson L. N., Mair G. A., North A. C. T., Phillips D. C., Sarma V. R. 1967; Crystallographic studies of the activity of hen egg-white lysozyme. Proceedings of the Royal Society B167:378–388
    [Google Scholar]
  3. Edebo L. 1961; Lysis of bacteria. 3. On the stability of protoplasts and spheroplasts in different pH-ranges. Acta Pathologica et Microbiologica Scandinavica 53:121–128
    [Google Scholar]
  4. Fermor T. R., Wood D. A. 1981; Degradation of bacteria by Agaricus bisporus and other fungi. Journal of General Microbiology 126:377–387
    [Google Scholar]
  5. Fouche P. B., Hash J. H. 1978; The N, O-diacetylmuramidase of Chalaropsis species. Identification of aspartyl and glutamyl residues in the active site. Journal of Biological Chemistry 253:6787–6793
    [Google Scholar]
  6. Ghuysen J. -M., Tipper D. J., Strominger J. L. 1966; Enzymes that degrade bacterial cell walls. Methods in Enzymofogy 8:685–699
    [Google Scholar]
  7. Grant W. D., Fermor T. R., Wood D. A. 1984; Production of bacteriolytic enzymes and degradation of bacterial cell walls during growth of Agaricus bisporus on Bacillus subtilis . Journal of General Microbiology 130:761–769
    [Google Scholar]
  8. Grant W. D., Rhodes L. L., Prosser B. A., Asher R. A. 1984; Production of bacteriolytic enzymes and degradation of bacteria by filamentous fungi. Journal of General Microbiology 132:2353–2358
    [Google Scholar]
  9. Grula E. A., Hartsell S. E. 1954; Lysozyme action and its relation to the Nakamura effect. Journal of Bacteriology 68:302–306
    [Google Scholar]
  10. Grula E. A., Hartsell S. E. 1957a; Lysozyme in the bacteriolysis of Gram-negative bacteria. I. Morphological changes during use of Nakamura’s technique. Canadian Journal of Microbiology 3:13–22
    [Google Scholar]
  11. Grula E. A., Hartsell S. E. 1957b; Lysozyme in the bacteriolysis of Gram-negative bacteria. II. Factors influencing clearing during the Nakamura treatment. Canadian Journal of Microbiology 3:23–34
    [Google Scholar]
  12. Hash J. H. 1963; Purification and properties of staphylolytic enzymes from Chalaropsis sp. Archives of Biochemistry and Biophysics 102:379–388
    [Google Scholar]
  13. Hash J. H. 1967; Measurement of bacteriolytic enzymes. Journal of Bacteriology 93:1201–1202
    [Google Scholar]
  14. Hash J. H. 1974; Lysozyme Chalaropsis . In Lysozyme pp. 95–103 Osserman E. F., Canfield R. E., Beychok S. Edited by New York London: Academic Press;
    [Google Scholar]
  15. Hash J. H., Rothlauf M. V. 1967; The N, O-diacetylmuramidase of Chalaropsis species. Journal of Biological Chemistry 242:5586–5590
    [Google Scholar]
  16. Hayashi K., Kasumi T., Kubo N., Sugimoto T., Tsumura N. 1983; Cultural conditions for lytic enzyme production by Streptomyces rutgersensis . Journal of Fermentation Technology 61:421–424
    [Google Scholar]
  17. Hayashi K., Kasumi T., Kubo N., Tsumura N. 1984; Properties of N-acetylmuramidase from Streptomyces rutgersensis H-46. Agricultural and Biological Chemistry 48:465–47!
    [Google Scholar]
  18. Hughey V. L., Johnson E. A. 1987; Antimicrobial activity of lysozyme against bacteria involved in food spoilage and food-borne disease. Applied and Environmental Microbiology 53:2165–2170
    [Google Scholar]
  19. Imada A., Nakahama K., Igarasi S., Isono M. 1973; A bacteriolytic enzyme from Chaetomium globosum, a marine isolate. Archiv für Mikrobiologie 91:41–54
    [Google Scholar]
  20. Van Iterson W., Op Den Kamp J. A. F. 1969; Bacteria-shaped gymnoplasts (protoplasts) of Bacillus subtilis . Journal of Bacteriology 99:304–315
    [Google Scholar]
  21. Maruhn D., Fuchs I., Mues G., Bock K. D. 1976; Normal limits of urinary excretion of eleven enzymes. Clinical Chemistry 22:1567–1574
    [Google Scholar]
  22. Morita T., Hara S., Matsushima Y. 1978; Purification and characterization of lysozyme produced by Streptomyces erythraeus . Journal of Biochemistry 83:898–903
    [Google Scholar]
  23. Nakamura O. 1923; Ueber Lysozymwirkungen. Zeitschrift für Immunitätsforschung und experimentelle Therapie 38:425–447
    [Google Scholar]
  24. Op Den Kamp J. A. F., Van Iterson W., Van Deenen L. L. M. 1967; Studies on the phospholipids and morphology of protoplasts of Bacillus megaterium . Biochimica et Biophysica Acta 135:862–884
    [Google Scholar]
  25. Paice M. G., Desrochers M., Rho D., Jurasek L., Roy C., Rollin C. F., De Miguel E., Yaguchi M. 1984; Two forms of endoglucanase from the basidiomycete Schizophyllum commune and their relationship to other β-1,4-glycoside hydrolases. Bio/technology 2:535–539
    [Google Scholar]
  26. Park J. T., Johnson M. J. 1949; A submicrodetermination of glucose. Journal of Biological Chemistry 181:149–151
    [Google Scholar]
  27. Rupley J. A., Banerjee S. K., Kreger I., Lapanje S., Shrake A. F., Turk V. 1974; Structure and chemistry of lysozyme: pH-rate profile, calorimetric studies, and computations on exposure to solvent. In Lysozyme pp. 251–267 Osserman E. F., Canfield R. E., Beychok S. Edited by New York & London: Academic Press;
    [Google Scholar]
  28. Seitz L. M., Sauer D. B., Burroughs R., Mohr H. E., Hubbard J. D. 1979; Ergosterol as a measure of fungal growth. Phytopathology 69:1201–1203
    [Google Scholar]
  29. Ward B. J., Perkins H. R. 1968; The purification and properties of two staphylolytic enzymes from Streptomyces griseus . Biochemical Journal 106:69–76
    [Google Scholar]
  30. Wecke J., Lahav M., Ginsburg I., Giesbrecht P. 1982; Cell wall degradation of Staphylococcus aureus by lysozyme. Archives of Microbiology 131:116–123
    [Google Scholar]
  31. Yaguchi M., Roy C., Rollin C. F., Paice M. G., Jurasek L. 1983; A fungal cellulase shows sequence homology with the active site of hen egg-white lysozyme. Biochemical and Biophysical Research Communications 116:408–411
    [Google Scholar]
  32. Yokogawa K., Kawata S., Yoshimura Y. 1973; Lytic enzyme from Streptomyces globisporus 1829 strain. Agricultural and Biological Chemistry 37:799–808
    [Google Scholar]
  33. Yokogawa K., Kawata S., Nishimura S., Ikeda Y., Yoshimura Y. 1974; Mutanolysin, bacteriolytic agent for cariogenic streptococci : partial purification and properties. Antimicrobial Agents and Chemotherapy 6:156–165
    [Google Scholar]
  34. Yokogawa K., Kawata S., Takemura T., Yoshimura Y. 1975; Purification and properties of lytic enzymes from Streptomyces globisporus 1829. Agricultural and Biological Chemistry 39:1533–1543
    [Google Scholar]
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