RT Journal Article SR Electronic(1) A1 Grant, W. D. A1 Prosser, B. A. A1 Asher, R. A.YR 1990 T1 A bacteriolytic muramidase from the basidiomycete Schizophyllum commune JF Microbiology, VO 136 IS 11 SP 2267 OP 2273 DO https://doi.org/10.1099/00221287-136-11-2267 PB Microbiology Society, SN 1465-2080, AB The basidiomycete Schizophyllum commune produces an extracellular bacteriolytic enzyme when grown on heat-killed cells of Bacillus subtilis as sole C, N and P source. The enzyme catalyses the dissolution of isolated B. subtilis cell walls at an optimum pH of 3·2–3·4, releasing muramyl reducing groups, which indicates that it is a muramidase. Although low levels of enzyme activity are present when the fungus is grown in the absence of bacteria, full enzyme production appears to be induced by bacterial cells and repressed by glucose. Whole bacteria are not lysed by the enzyme at pH 3·3, but are rendered osmotically fragile, and lyse when the pH is raised to 7 or higher. The muramidase is effective against several Gram-positive bacteria but did not lyse any of the Gramnegative species tested., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-136-11-2267