SUMMARY: The basidiomycete produces an extracellular bacteriolytic enzyme when grown on heat-killed cells of as sole C, N and P source. The enzyme catalyses the dissolution of isolated cell walls at an optimum pH of 3·2-3·4, releasing muramyl reducing groups, which indicates that it is a muramidase. Although low levels of enzyme activity are present when the fungus is grown in the absence of bacteria, full enzyme production appears to be induced by bacterial cells and repressed by glucose. Whole bacteria are not lysed by the enzyme at pH 3·3, but are rendered osmotically fragile, and lyse when the pH is raised to 7 or higher. The muramidase is effective against several Gram-positive bacteria but did not lyse any of the Gramnegative species tested.


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